Fig. 3

LCAT3 promotes proliferation of lung cancer cells both in vitro and in vivo. A qRT-PCR analysis of LCAT3 expression in Calu1 and Hop62 cells transfected with LCAT3 siRNA (siRNA2 or siRNA4) and negative control (NC) siRNA. B CCK-8 assays were performed to assess cell proliferation in NC siRNA and LCAT3 knockdown lung cancer cell lines. C Colony formation assays were performed to assess colony formation abilities in NC siRNA and LCAT3 knockdown lung cancer cell lines. D The number of colonies were counted from three independent assays. E EdU assays of Calu1 and Hop62 cells transfected with NC siRNA and LCAT3 siRNA. F The DNA replication ratio was determined from three independent assays. G Representative images of the xenograft tumors in mice from LCAT3 knockdown and control groups. Calu1 cells stably transfected with shLCAT3 or scramble control were subcutaneously inoculated into the flanks of mice. H, I Quantification of tumor volume (H) and tumor weight (I) in xenograft mouse models from LCAT3 knockdown and control groups