Fig. 1

7 × 19 CAR-T cells showed enhanced antitumor activity in xenografts. a Schemes of a lentiviral vector of anti-GPC3 CAR, anti-MSLN CAR and anti-CD20 CAR. IL-7 and CCL19 (7 × 19) were linked through the 2A peptide, and truncated CD19 was used to indicate 7 × 19 expression. b The percentage of CAR and 7 × 19 expression on transduced human T cells was analyzed by flow cytometry. c A total of 1 × 10⁶ T cells from each group were cultured in fresh media for 24 h. The supernatant was collected, and the secretion of IL-7 and CCL19 was analyzed by ELISA. The anti-IL-7R antibody and anti-CCR7 antibody were used during culture. Open circle represents the values of individual well. Error bar denotes the S.D. d A total of 1 × 10⁶ anti-GPC3-7 × 19 CAR-T cells or anti-GPC3 CAR-T cells were cultured in the lower chamber of the Transwell plate for 24 h, and then the anti-GPC3 CAR-T cells labeled with CSFE were cultured in the upper chamber for 5 h. The responder T cells that migrated from the upper chamber to the lower chamber were enumerated by flow cytometry. Error bar denoted the S.D. and the results were compared with an unpaired t-test. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. e A total of 2 × 10⁶ transduced T cells from each group were cultured in fresh media. The cell number was counted every two days. Error bar denotes the S.D. The results were compared with two-way ANOVA. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. f Tumor volume of HepG2 subcutaneously inoculated mice. Tumor volume = (length × width²)/2. g Tumor weight of HepG2 subcutaneously inoculated mice. h Percentage of T cells in peripheral blood of HepG2 subcutaneously inoculated mice. i Tumor volume of AsPC-1 subcutaneously inoculated mice. j Tumor weight of AsPC-1 subcutaneously inoculated mice. k Percentage of T cells in peripheral blood of AsPC-1 subcutaneously inoculated mice. f, i Error bar denotes the S.D. The results were compared with two-way ANOVA. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. g, h, j, k The error bar denotes the SD. The results were compared with ordinary one-way ANOVA. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001