Fig. 4

Ab@Tf-Cou6-PLGA NPs suppressed proliferation and induced apoptosis of CML cells. a The effect of nanoparticles on CML cells was tested by CCK-8 assay. b The number of colonies was counted. Scale bar, 100 μm. c The apoptosis rate of CML cells was detected by FCM. d Morphologic changes of apoptosis were tasted by DAPI staining. The typical apoptosis cells were pointed out by arrows. Scale bar, 10 μm. e The expression of cleaved PARP and cleaved caspase-3 was assessed by western blot assay. Data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001