Fig. 6

Flow cytometry and immunofluorescent staining assays to analyze the status of CD8⁺ tumor-infiltrating lymphocytes (TILs) in the B16 model. The representative images of tumor-infiltrating a CD8⁺ T cells, b Ki67⁺ CD8⁺ T cells, c Granzyme-B⁺ CD8⁺ T cells, d CD107a⁺ CD8⁺ T cells, e CD69⁺ CD8⁺ T cells, f CD25⁺ CD8⁺ T cells, and g CD44⁺ CD8⁺ T cells in flow cytometry assays. h The representative images of tumor-infiltrating CD4⁺ and CD8⁺ T cells in immunofluorescent staining assays (n = 5 biological replicates). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 denote the significant difference relative to combination treatment