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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: SIRPα-αCD123 fusion antibodies targeting CD123 in conjunction with CD47 blockade enhance the clearance of AML-initiating cells

Fig. 1

SIRPα-αCD123 fusion antibodies bind to CD123 and CD47. A Structure of SIRPα-αCD123 fusion antibodies. VH—variable heavy, CL—constant light, CH1—constant heavy 1, CH2—constant heavy 2, CH3—constant heavy 3. B Different CD123 concentrations binding to the antibody constructs measured using SPR. Raw data are shown in red; black curves were fitted to a 1:1 interaction. KD values represent mean values from n = 3 independent experiments ± standard error of the mean (SEM). C Binding of antibodies to CHO_hCD47+ and CHO_hCD47 cells at 100 nM concentration measured by flow cytometry. The grey line indicates nonspecific staining of the isotype control and secondary antibody. Histograms show 1 of 3 experiments with similar results

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