Fig. 2

CircHERC4 was overexpressed in CRC tissues and associated with metastasis. a Relative RNA level of circHERC4 in CRC tissues and paired adjacent normal tissues, ***, P < 0.001. b Relative RNA level of circHERC4 in CRC tissues with lymphatic metastasis compared to those without lymphatic metastasis, **, P < 0.01. c Relative RNA level of circHERC4 in CRC tissues with distant metastasis compared to those without distant metastasis, **, P < 0.01. d Relative RNA level of HERC4 mRNA in CRC tissues and paired adjacent normal tissues, ns: no significance. e No significant changes were found in HERC4 mRNA expression between CRC tissues with and without lymphatic metastasis. ns: no significance. f No significant changes were found in HERC4 mRNA expression between CRC tissues with and without distant metastasis. ns: no significance. g Kaplan–Meier analysis of the association between circHERC4 expression and CRC patient overall survival (OS) in the 64-patient cohort. h Data from TCGA showed no significant association between HERC4 mRNA expression and CRC patient OS, ns: no significance