Fig. 5

CTBP2 was a target gene of miR-556-5p. a RNA-seq was used to predict the target gene of miR-556-5p in CRC; the venn diagram showed that 9109 transcripts were changed together in three groups. b KEGG pathway analysis demonstrated that Notch signaling system was involved and might be the downstream of circHERC4 and miR-556-5p. c Heat map was performed to analyze the changed genes that were contained in the Notch signaling system. NM 001321013.1: CTBP2. d Upper, predicted binding sites between miR-556-5p and CTBP2. Lower, luciferase reporter experiment in DLD-1 co-transfected with miR-556-5p mimics and psi-check2-mutant type CTBP2 plasmid compared to psi-check2 wild-type CTBP2 plasmid, ***, P < 0.001. e Left, Western blotting showed that CTBP2 was downregulated and E-cadherin was upregulated when miR-556-5p was overexpressed. Right, knockdown of miR-556-5p inhibited CTBP2 and activated E-cadherin