Fig. 6

CTBP2 was overexpressed in CRC tissues and could promote the progression of CRC cells. a GEPIA database showed that CTBP2 was significantly upregulated in CRC tissues. b Kaplan–Meier analysis of survival probability from The Human Protein Atlas database showed that higher CTBP2 expression was associated with shorter survival time. c IHC staining of CTBP2 in clinical CRC tissues and matched adjacent normal tissues, n = 120, ***, P < 0.001. d Efficiency of CTBP2 siRNA was detected by Western blotting. e–g CCK8, colony formation and transwell assays presented that knockdown of CTBP2 could significantly repress the proliferation, migration and invasion of DLD-1 and HCT116 cells, ***, P < 0.001. h Western blotting showed that CTBP2 was downregulated and E-cadherin was upregulated when circHERC4 was silenced. i IHC staining was used to detect CTBP2, E-cadherin and Ki67 expression in sh-NC, sh-circHERC4-1 and sh-circHERC4-2 HCT116 group. j–l Schemes revealed that oncogenic function of circHERC4 was rescued by downregulation of CTBP2, *, P < 0.05, **, P < 0.01, ***, P < 0.001. m Western blotting was performed to reveal that overexpression of CTBP2 could partially rescue the effect of silenced circHERC4 on the expression of E-cadherin