Fig. 2
Clinical outcomes analysis according to the FHC across the pembrolizumab and chemotherapy matched cohorts. Median OS and PFS of the entire pembrolizumab cohort were 15.4 months (95% CI 12.8–17.3; 421 events) and 6.9 months (95% CI 5.8–7.9; 523 events), respectively, whilst for the chemotherapy cohort were 14.4 months (95% CI 12.9–16.6; 466 events) and 5.9 months (95% CI 5.3–6.3; 594 events), respectively. The median follow-up was 23.3 months (95% CI 21.8–38.0) for the pembrolizumab cohort and 38.4 months (95% CI 33.1–86.7) for the chemotherapy cohort. Kaplan–Meier survival estimates for OS; pembrolizumab cohort (A) FHC-high: 31.3 months (95% CI 15.2–31.3, 21 events) vs FHC-low/negative: 15.3 months (95% CI 12.8–17.5, 327 events), p = 0.0281; chemotherapy cohort (C) FHC-high: 16.9 months (95% CI 12.1–34.5, 29 events) vs FHC-low/negative: 13.8 months (95% CI 12.3–15.8, 408 events), p = 0.0866. Kaplan–Meier survival estimates for PFS; pembrolizumab cohort (B) FHC-high: 17.2 months (95% CI 8.6–28.1, 28 events) vs FHC-low/negative: 6.5 months (95% CI 5.4–28.3, 405 events), p = 0.0074; chemotherapy cohort (D) FHC-high: 5.9 months (95% CI 3.9–6.9, 44 events) vs FHC-low/negative: 5.0 months (95% CI 5.3–6.4, 5090 events), p = 0.7039. DCR; pembrolizumab cohort (E) FHC-high: 86.4% (95% CI 61.1–118.5) vs FHC-low/negative: 67.5% (95% CI 60.5–75.1), p = 0.0096; chemotherapy cohort (F) FHC-high: 69.7% (95% CI 44.1–104.5) vs FHC-low/negative: 63.1% (95% CI 56.0–70.1), p = 0.4475. (G) OncoPrint plot summarizing relevant baseline clinic-pathologic characteristics and the DDR genes profile of the parallel cohort. Patients are clustered according to the FHC status (first row) and in the upper section the smoking status, common actionable biomarkers (including EGFR, ALK and ROS-1), the PD-L1 tumour expression and the TMB category (with a cut off of ≥ vs < 10 mutations/megabase) are reported. The mutational status and its prevalence of selected DDR genes is reported with different colours according to the mutation’s type. Made with cBioPortal oncoprinter, available at: https://www.cbioportal.org/oncoprinter. FHC, family history of cancer; OS, overall survival; PFS, progression free survival; DCR, disease control rate