Fig. 1
From: Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis
Schematic diagram of single-cell sequencing. First, solid tumor samples are processed to separate the surviving single cells A–B. Then, the single cells are lysed to obtain DNA or RNA, reverse-transcribed into cDNA, and then amplified to construct a sequencing library C. Once the sequencing library has been prepared, the critical single-cell sequencing steps are performed on a sequencing platform (D). After sequencing, it is necessary to visualize and interpret those data E–F