From: Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis
Method | Flux | Advantages | Disadvantages | Ref |
---|---|---|---|---|
Smart-seq2 | Low | a. High sensitivity, high transcription coverage b. Cell capture visualization c. Analysis of rare cell populations | a. No early multiplexing b. Longer cycle | |
CEL-Seq2 | Low | a. Higher sensitivity, lower cost b. Lower hands-on input | a. Strong 3' preference b. High-abundance transcripts are preferentially amplified | 30] |
10x- Chromium | High | a. Less time-consuming and low technical noise b. Analysis of rare cell population | a. There are too many steps for DNA library construction b. Higher sample requirement | |
Drop -seq | High | a. Low cost and fast b More effective | Lower cell capture efficiency | 35] |
Seq-Well | High | Easy-to-use, portable, low cost b. Efficient cell lysis and transcriptome capture | a. Lower cell capture efficiency | 35] |
In Drops | High | a. Lower cost b. Strong cell capture and simplification capabilities | a. Extremely lower cell capture efficiency | |
Sci-RNA-seq | High | a. Minimize perturbation to RNA integrity | a. Some cell types cannot be defined | [30] |