Table 3 Potential application of single-cell sequencing in human cancers
From: Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis
Tumor type | Sample type | Number | Detection method | Clinical significance | Ref |
|---|---|---|---|---|---|
Colorectal cancer | Tissues | 1 patient | scRNA-seq | The study found a total of 12 clusters corresponding to 6 cell types were identified from patient sample of CRC liver metastasis | [122] |
Breast cancer | Tissues | – | scRNA-seq | The study elucidated role of stem cell-like clone subset in breast cancer metastasis | [128] |
| Â | Cells | 55 patients | scRNA-seq ATAC-seq | This study provided a new therapeutic target for breast cancer lymph node metastasis | [127] |
|  | Tissues | – | scRNA-seq | The study revealed the main pathway of tumor metastasis upregulation is mitochondrial oxidative phosphorylation | [80] |
| Â | Blood | 5 patients | scRNA-seq | The study may provide a key therapeutic target for breast cancer metastasis | [131] |
|  | Blood | – | scRNA-seq | The study identified a rare but highly metastatic subpopulation of CTCs | [9] |
Adenocarcinoma | Cells | 44 patients | scRNA-seq | The study showed that ts2-specific related genes are associated with lung adenocarcinoma progression and metastasis | [41] |
Pancreatic cancer | Blood | 168 single CTCs | scRNA-seq | The study found the SPARC gene is highly expressed in pancreatic CTCs, which may provide a novel target for the therapy of pancreatic cancer | [132] |
Prostate cancer | Tissues | 10 patients | scRNA-seq | The study showed that mutations in androgen receptor signaling reveals unprecedented, detailed transfer mechanisms | [124] |
Metastatic melanoma | Tissues | 19 patients | scRNA-seq | The study showed the mechanism of T lymphocyte activation and cloning in this tumor tissues | [129] |
Clear cell renal cell carcinoma | Tissues | 121 cells | scRNA-seq | The study found and identified 44 metastasis-associated marker genes | [130] |