Fig. 1

IL1RAP is overexpressed in pancreatic cancer. A, B: Tissue microarrays containing human pancreatic ductal adenocarcinoma (PDAC) samples were stained with antibodies against IL1RAP and examined by immunohistochemistry. Representative scores for both TMAs are shown. Majority (81%) of PDAC samples demonstrated positivity for IL1RAP expression. C–E: Tissues from wildtype mouse pancreas (C), preneoplastic lesions (D) and pancreatic cancer (E) in KRAS mutant, TP53 mutant (KPC) mouse model were obtained and stained with antibody against IL1RAP. Increased membranous intensity of IL1RAP expression was seen in PDAC samples from KPC mouse. F–H: Single cell RNAseq done on samples from human pre-neoplastic lesions (IPMN, F), High risk IPMN (G) and advanced PDAC (H) tumors was examined for IL1RAP expression (shown in purple). The percentage of IL1RAP positive cells is expressed per total EPCAM positive cells. I: Analysis of 176 PDAC samples (TCGA) shows that higher expression of IL1RAP is associated with worse overall survival. High and low expression based on median, KM curve with log rank P value = 0.012. J: IL1RAP expression in PDAC derived cell lines and ductal control (HPDE) shows increased expression in A6L and other cell lines. K: siRNA mediated reduction in IL1RAP expression is seen in A6L PDAC cells by flow cytometry. L: siRNA mediated knockdown of IL1RAP leads to reduced viability in A6L PDAC cells. Means ± stdev, N = 8. Ttest P Val < 0.01. M: siRNA mediated knockdown of IL1RAP leads to reduced colony growth in A6L PDAC cells. Means ± stdev, N = 10 P Val < 0.01. N: siRNA mediated knockdown of IL1RAP leads to reduced invasiveness in A6L PDAC cells. Means ± stdev, N = 12. Ttest P Val < 0.01