Fig. 6

Proof-of principle showing the high sensitivity of LIFE-CNA. Focal SCNAs (foc. SCNA), tumor fraction (tum. frac.), tumor fraction in 90 to 150 bp fragments(tum. frac. short), enrichment in fragments from 90 to 150 bp (glob. frag.), differential regional fragmentation (reg. frag.), significantly stronger coverage drop in at least to region sets (low cov.), classifier based on global fragmentation (ML glob. frag.), classifier based on regional fragmentation (ML reg. frag.), and classifier based on meta-learner (ML Meta.) were analyzed in six additional healthy controls not included in the panel of normals and in in silico dilutions with 0.5%, 1%, 2.5%, 5% and 10% tumor fraction as a proof-of-principle for ctDNA detection using LIFE-CNA