Fig. 7

LINC01614 correlates with glutamine transporters and poor survival in patients with LUAD. A Representative ISH for LINC01614 and IHC for α-SMA in a TMA cohort containing 78 paired LUAD tissues and adjacent normal tissues. Scale bars, 500 μm (×100 magnification), 100 μm (×400 magnification) (n = 78). B–D The CISH results of the TMA. B The expression of LINC01614 was upregulated in the α-SMA High expression (high CAFs infiltration) group (≥ 6, median). C, D The expression level of LINC01614 in LUAD tissues was positively correlated with the T stage and TNM stage in the TMA cohort. E Higher expression (≥ 6, median) of LINC01614 in LUAD tissues was associated with poor prognosis in the TMA cohort. F Univariate and multivariate Cox regression analyses indicated that high expression of LINC01614 in LUAD tissues was an independent prognostic factor for poor survival (n = 78). G Representative H&E staining, ISH for LINC01614, and IHC for α-SMA, SLC38A2, and SLC7A5 in LUAD and adjacent normal tissues (n = 10). H Correlation between LINC01614 expression in CAFs and SLC38A2 in tumor cells from the same patients (n = 15). I Correlation between LINC01614 expression in CAFs and SLC7A5 in tumor cells from the same patients (n = 15). J Graphical illustration of the interaction between CAFs and LUAD cells. For B–D, the mean ± s.d. are shown, and Student’s t-test determined P values. For H, I Spearman correlation analysis was performed. **P < 0.01, ***P < 0.001, ****P < 0.001. LUAD, lung adenocarcinoma