Fig. 1

CD123 is highly expressed on primary blasts collected from paediatric AML patients. A Percentage of CD123 expression levels in primary BM cells obtained from AML patients (triangles) and HD (circles) were assessed by flow cytometry analysis, across 3 main haematopoietic cell subpopulations, namely the mature CD38⁺CD34⁻ cells, the intermediate CD38⁺ CD34⁺ cells, and the most immature CD38⁻ CD34⁺ stem cells. The gating strategy is shown in Additional file 1: Fig. S1. B Median fluorescence intensity (MFI) for CD123 on AML primary blasts or BM-derived cells from HDs. MFI was adjusted for cell size by dividing MFI by the forward scatter (FSC). C Geometric mean fluorescence intensities (GeoMean) of CD123 on AML primary blasts or BM-derived cells from HDs. Data are shown as average ± SD. *p < 0.05, **p < 0.01, ***p ≤ 0.001. D The heatmap shows the CD123 MFI detected on HSC precursors from BM of AML patients and HDs