Fig. 7

In vitro and in vivo on-target off-tumour effect of both CAR.CD123-T and CAR.CD123-NK cells against hematopoietic precursors. A Colony-forming unit (CFU) assay was performed with CD34⁺ cells derived from BM samples of 3 HDs after a short-term 4-h coculture with media alone (control condition, CNT; black line bars), un-transduced T cells (NT-T; black bars), CAR.CD123-T (green bars), un-transduced NK cells (NT-NK; blue bars) or CAR.CD123-NK cells (red bars). BFU-E and CFU-GM colonies were counted after 14 days. Results are shown as average of absolute count ± SD. B Schematic representation of humanized mouse model of hGM-CSF/hIL3 NOG (hu-NOG) mice engrafted with CD34⁺ cells derived from cord blood (CB) and infused with effector cells. C 15-Day probability of OS for humanized mouse model engrafted with adoptive transfer of NT-T (black line with circle), CAR.CD123-T (dotted green line with squares), NT-NK (blue line with rhombus) and CAR.CD123-NK (red line with triangles) cells. D Flow cytometry analysis of hCD45 cells in PB of hu-NOG mice before (grey bars) and (squared pattern bars) after 4 days from the effector cells infusion. Graphs show the percentage of hCD45⁺ cells on the total mononuclear cells present in PB (left panel), and the percentage of hCD123⁺ cells on the total hCD45⁺ cells present in the PB samples (right panel). Data are shown as average of ± SD. E–F Flow cytometry analysis of human hematopoietic cell quantification in tibia BM (E) and spleen (F) of hu-NOG mice receiving NT-T (black bars), CAR.CD123-T (green bars), NK-NT (blue bars) or CAR.CD123-NK (red bars). Graphs report the percentage of hCD45⁺ cells on the total mononuclear cells present in the investigated tissue (first panel), the percentage of hCD34⁻CD38⁺ cells on the total hCD45⁺ cells (second panel), the percentage of hCD34⁺CD38⁺ cells on the total hCD45⁺ cells (third panel), and the percentage of hCD34⁺CD38⁻ cells on the total hCD45.⁺ cells (fourth panel). Data are shown as average ± SD. *p < 0.05, **p < 0,001