Fig. 4

miR-455-5p modulates cisplatin resistance in vitro and in vivo. A qRT-PCR of miR-455-5p in NCI-H1993 transfected with a miR-455-5p mimic (NCI-H1993 OE) or a negative control mimic (NCI-H1993 CTRL). Data, expressed as normalized Cq (Cqn), are mean ± SEM (N = 5). P value was calculated by the Mann–Whitney U test. B Dose–response curves of NCI-H1993 CTRL and NCI-H1993 OE cells treated with cisplatin for 72 h. Error bars indicate SEM (N = 4). C Bar plot of cisplatin potency and efficacy of NCI-H1993 CTRL and NCI-H1993 OE cells. Data are mean ± SEM (N = 4). Fold change is relative to NCI-H1993 CTRL. P value was calculated by one sample t test. *P < 0.05; ns, not significant. D Generation of a model of in vitro acquired cisplatin resistance. E qRT-PCR of miR-455-5p in Parental and CDDP-R cell lines. Data, expressed as Cqn, are mean ± SEM (N = 4). P value was calculated by t test with Welch’s correction. F qRT-PCR of miR-455-5p in Parental and CDDP-R transfected either with a miR-455-5p mimic (i.e., Parental OE and CDDP-R OE) or a negative control mimic (i.e., Parental CTRL and CDDP-R CTRL). Data, expressed as Cqn, are mean ± SEM (N = 4). P value was calculated using the Mann–Whitney U test. *P < 0.05. G Dose–response curves of indicated cell lines treated with cisplatin for 72 h. Error bars indicate SEM (N = 5). H Bar plot of cisplatin potency and efficacy of Parental CTRL, Parental OE, CDDP-R CTRL and CDDP-R OE cells. Data are mean ± SEM (N = 5). Fold change is relative to CTRL. P value was calculated by one sample t test. **P < 0.01; ns, not significant. I Schematic representation of zCDX model to monitor chemotherapy response in vivo. J Representative fluorescence images of zebrafish larvae injected with tumor cells. Dil (red) identifies tumor cells; eGFP (green) visualizes blood vessels. Scale bar: 200 μm. K Representative fluorescence images of tumor masses upon 3 days of cisplatin treatment. Dil (red) identifies tumor cells. Scale bar: 200 μm. L Size distribution of tumor masses derived from indicated cell lines. Columns represent mean ± SEM (N = 16–20, for each condition). Results are shown as relative tumor size (i.e., percent change in tumor size by comparing day 4 vs. day 1). Effect size is expressed as percent reduction in mean value of tumor size. P values were calculated by the Mann–Whitney U test