Fig. 5

Recognition of healthy hematopoietic and non-hematopoietic subsets by Jchain TCR-transduced CD8 T cells. A IFN-γ production after overnight co-culture of Jchain TCR Td CD8 T cells with CD40L activated B cells, immature dendritic cells (immDCs), mature dendritic cells (mDCs), PHA-activated T cells (PHA T cells), and keratinocytes or fibroblasts pre-treated for 48 h with 100 IU/ml IFN-γ. Symbols represent the average value (from technical duplicates) of target cells isolated from different donors. Target cells not expressing the relevant HLA restriction allele are depicted in gray, cells expressing the HLA restriction alleles are depicted in color. Per panel T cells with one of the Jchain TCRs are shown as indicated in the graph titles. K562 + HLA and peptide loaded K562 + HLA are included as negative and positive controls. B, C FACS-based killing experiment of CD40L activated peripheral blood B cells from healthy donors with Jchain A1 and Jchain A24 TCR T cells in an E/T ratio of 3:1, samples were measured using fixed acquisition times and fixed flow rates. B Example of B cell survival and killing after overnight co-culture of HLA-A1^neg/HLA-A24^pos B cells with CMV TCR CD8 T cells (left), Jchain A1 TCR T cells (middle) and Jchain A24 TCR T cells (right). Gated on SYTOX blue-, single cells, CD3-, CD19 + . C Quantification of percentage surviving B cells of data in B and additional donors, HLA-A1 and -A24 typing is indicated in graph titles. Percentage surviving cells was calculated relative to cells in the negative control CMV TCR T-cell culture. Technical triplicates are shown