Fig. 2
Ferroptosis occurs in the wasting tissues of mice with lung cancer cachexia. A. Mice were inoculated with LLC cells to induce the murine cachexia, and eWAT tissues from cachectic and control mice were harvested on day 21 and analyzed by RNA-seq; this panel shows a heatmap of differentially expressed genes (DEGs) in the eWAT tissue. B. KEGG pathway enrichment analysis. C. Heatmap analysis showing the normalized expression of ferroptosis-related genes. D. Gene set enrichment analysis (GSEA) showing that metabolic processes involving reactive oxygen species were enriched in the cachectic mice compared with controls. E. qPCR analysis of the indicated ferroptosis gene mRNA levels in the eWAT, iWAT, and Gast. n = 6 per group. F. Chemiluminescence analysis of Fe2+ concentrations in the eWAT, iWAT, and Gast. n = 5–6 per group. G. Chemiluminescence analysis of MDA concentration in the eWAT, iWAT, and Gast. n = 5–6 per group. Data are shown as the mean ± SEM. Statistical analyses were performed using unpaired Student’s t-tests (E, F, G)