Fig. 3
From: GAS6-based CAR-T cells exhibit potent antitumor activity against pancreatic cancer
GAS6-CAR-T cells cytotoxicity is abolished by silencing AXL. A The knockdown efficiency of shRNAs targeting AXL, TYRO3, or MERTK was tested by flow cytometry. Cytotoxicity of GAS6-CAR-T cells on target cells PANC1-shRNA (B) and MIA PaCa2-shRNA (C) was tested at an E/T ratio of 4:1 for 24 h (n = 3). ELISA-based quantification of IFN-γ (D& E) and TNF-α (F& G) release in response to coculture with Mock T cells or GAS6-CAR-T cells