Fig. 6

Immunofluorescence staining to measure the status of epithelial-mesenchymal transition (EMT) of cancer cells, carcinoma-associated fibroblast (CAF) and tumor angiogenesis in H22 tumor model. The representative images and quantitative analysis of a–e EMT-related markers, including anti-E-cadherin staining, anti-Vimentin staining, anti-N-cadherin staining, f CAF marker, anti-α-SMA staining, g Anti-CD31 staining. The size of the scale bar in the immunofluorescence images refer to 100 μm. Bars, SDs; ***p < 0.001, and ****p < 0.0001 denote the significant difference relative to Y332D treatment. ns: not significant, α-TGF-β: anti-TGF-β, α-VEGF: anti-VEGF