Fig. 8

RNA-seq assay to explore the immune profile of H22 tumors after different treatments. a Heat map to represent the differentially expressed genes among four groups. b Heat map to represent the expression levels of cytotoxicity-related genes (Gzma, Gzmb, Prf1, Ifng, Tnf, etc.). c Heat map to represent the expression levels of chemokines (Ccl11, Cxcl9, Cxcl11, Cxcl12, Cxcl16). d, e Heat map to represent the expression levels of signature genes in T cells and NK cells, and signature scores were calculated to quantify. f–h The top 10 significantly enriched immune-related Gene Ontology (GO) terms (α-PD-1 + Y332D vs. Vehicle; α-PD-1 + Y332D vs. α-PD-1; α-PD-1 + Y332D vs. Y332D). i, j Quantitative RT-PCR validation of selected genes identified by RNA-seq. Bars, SDs; *p < 0.05 and **p < 0.01 denote the significant difference relative to combination treatment. α-PD-1: anti-PD-1