Fig. 2

Single nucleotide variants in genes found in cfDNA of PTLD patients. A Waterfall plot displaying types of mutations in each plasma sample for each gene. Top- and right-side bar plots show total number of mutations found in a sample (tumor mutation burden, TMB) or a gene, respectively. Genes are sorted based on this number. Samples are grouped by Epstein Bar virus (EBV) status. EBV status, Ann-Arbor staging, Estimated tumor fraction (ETF) and fraction of genome altered (FGA) information is shown below. Pathway information per gene is indicated by color, left of the gene names. B A significant correlation between SNV load and lactate dehydrogenase (LDH) was observed. Dashed line represents cut-off value at 248 U/L discriminating clinically elevated LDH from normal LDH value. C Correlation between SNV load and metabolic tumor volume (MTV). In panels B and C, the grey areas around the regression lines represent the 95% CI and the Spearman coefficient is indicated with ρ. D The total number of SNVs per sample is shown, grouped by EBV status. EBV-negative samples show a slightly higher mean, though this difference is insignificant as tested by Wilcoxon Signed Rank test