Fig. 4

Tumor kinetics and survival of subcutaneous CD70⁺ Raji-bearing mice after treatment with CD70-CAR NK cells and CD70-CAR-IL-15 NK cells. A Schematic representation of (top) production of MOCK NK cells, CD70-CAR NK cells, and CD70-CAR-IL-15 NK cells prior to injection, and (bottom) schedule of generation and treatment of the CD70⁺ Raji xenograft mouse model. B Representative images of FFPE-slides of a Raji tumor stained for H&E and for CD70 positivity. Raji-bearing mice were treated twice with three days in between with 1.0 × 10⁷ MOCK NK cells (n = 8), CD70-CAR NK cells (n = 6) or CD70-CAR-IL-15 NK cells (n = 6). Untreated mice were included as control (n = 8). C Tumor kinetics over time post-treatment; red arrows indicate treatment days. Error bars represent mean values ± standard error of mean. D Survival curve post-treatment. E Separated spider plots of the tumor kinetics over time per treatment group. Mixed model ANOVA was used to compare differences in tumor kinetics and the Log-Rank (Mantel-Cox) test was performed to analyze differences in survival. *p < 0.05, **p < 0.01 and ***p < 0.001