MicroRNAs and Toll-like Receptor/Interleukin-1 Receptor Signaling
© Virtue et al.; licensee BioMed Central Ltd. 2012
Received: 18 September 2012
Accepted: 15 October 2012
Published: 18 October 2012
The discovery of miRNAs has revolutionized the way we examine the genome, RNA products, and the regulation of transcription and translation. Their ability to modulate protein expression through mRNA degradation and translation repression resulted in avid scientific interest in miRNAs over the past decade. This research has led to findings that indicate miRNAs can regulate an array of cellular functions such as cellular apoptosis, proliferation, differentiation, and metabolism. Specifically, the capability of miRNAs to finely-tune gene expression naturally lends itself to immune system regulation which requires precise control for proper activity. In fact, abnormal miRNAs expression is often seen with inflammatory disorders like rheumatoid arthritis, systemic lupus erthematosus, experimental autoimmune encephalomyelitis, and inflammatory cancers. As a result, research investigating miRNAs modulation of immune cell proliferation, differentiation, and cellular signaling has yielded fruitful results. Specifically, in this review, we will examine the impact of miRNAs on toll-like receptor (TLRs) and interleukin-1β (IL-1β) signaling, which are integral in the proper functioning of the innate immune system. These signaling pathways share several key downstream signaling adaptors and therefore produce similar downstream effects such as the production of pro-inflammatory cytokines, chemokines, and interferons. This review will examine in depth the specific interactions of miRNAs with receptors, adaptor molecules, and regulator molecules within these cellular pathways. In addition, we will discuss the modulation of miRNAs’ expression by TLR and IL-1R signaling through positive and negative feedback loops.
KeywordsMicroRNAs Toll-like receptors Interleukin-1 receptor mRNA stability Inflammation
The existence of a molecular system capable of orchestrating the expression of numerous proteins was postulated by researchers for some time; the discovery of microRNAs (miRNAs) confirmed this hypothesis. This breakthrough revolutionized the way we examine the genome organization, RNA products, as well as mRNAs translational and degradation. Originally discovered in 1993, it soon became evident that miRNAs are abundant and often conserved across species. Even more striking is their ability to modulate a wide range of cellular functions, such as apoptosis, proliferation, differentiation, and metabolism. In addition to cellular activities, miRNAs were also found to be capable of regulating a broad spectrum of systemic functions like inflammatory responses. The nature of miRNAs function, to finely-tune protein expression through mRNAs degradation and translation repression, naturally lends itself to immune system regulation which requires precise control for proper activity. Therefore, it is not coincidence that altered miRNAs expression is often associated with progression and remission of inflammatory disorders like rheumatoid arthritis, systemic lupus erythematosus, and experimental autoimmune encephalomyelitis. Ensuing research investigating miRNAs modulation of immune cell proliferation, differentiation, activity, and cellular signaling has yielded fruitful results. Specifically, in this review, we will examine the impact of miRNAs on the integral toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) inflammatory signaling pathways. Both pathways share several key signaling adaptors and therefore induce similar cellular responses when activated. Together they play an indispensable role in the innate immune system by triggering the production of pro-inflammatory proteins like cytokines, chemokines, and interferons. The specific interactions of miRNAs with receptors, adaptor molecules, and regulator molecules within these cellular pathways will be examined in depth. Finally, the modulation of miRNAs expression by TLR and IL-1R signaling will be reviewed.
Prior to recent breakthroughs, it was believed that the majority of DNAs were transcribed to mRNAs and then translated into proteins. The remaining RNAs not coding for protein were either ribosomal RNAs, transfer RNAs, or perceived as “junk” with little to no pertinent function. In 2003, at the completion of the Human Genome Project, between 20,000 – 25,000 protein coding genes were identified from the 3 billion nucleotide base-pairs of the human genome. Puzzling was the fact that protein coding sequences only accounted for approximately 2% of the total genome . These findings made it hard to believe that such a large majority of the genome was “junk”. Partial enlightenment came with the discovery of additional classes of non-coding RNAs (ncRNAs). Defined simply, ncRNAs are any RNAs that are not translated into protein. Functional applications of these once over looked segments of RNAs include but are not limited to cell metabolism, cell proliferation, cell differentiation, protein secretion, and embryonic development [2–5]. Several classes of ncRNAs exist including transfer RNAs (tRNAs), small nuclear RNAs (snRNAs), ribosomal RNAs (rRNAs), small interfering RNAs (siRNAs), and microRNAs (miRNAs). Possibly the most intriguing class of ncRNAs is miRNAs whose discovery dramatically revised the dogma that RNAs simply act as a template for protein translation. Mature miRNAs are capable of post-transcriptional gene silencing through base-pair interactions with mRNAs and therefore can regulate protein expression. Thus far, over 1,000 miRNAs have been identified which are predicted to regulate up to 30% of protein encoding genes.
miRNAs:mRNAs interactions usually occur at the 3’ untranslated region (3’UTR) of mRNAs through imperfect Watson and Crick base-pairing. Noteworthy is that imperfect pairing facilitates the possibility of multiple binding sites for a single miRNA with a particular mRNA. It also promotes promiscuity allowing for multiple mRNA partners for a single miRNA. However, the molecular details of how miRNAs specifically target mRNAs temporally and dynamically are still unknown. Despite this, several structural features which promote miRNAs:mRNAs binding have been elucidated. It has been determined that a region of 6–8 nucleotides, at residues 2–7 on the 5’ end of miRNAs, is critical in establishing miRNAs:mRNAs interactions and is commonly referred to as the “seed region” . The significance of this region is supported by the high level of conservation at the 5’ ends of related miRNAs [9–12]. Meanwhile, the 3’ ends of miRNAs have been demonstrated to participate in base-pairing as well. With the ability to contribute to mRNAs interference, it is postulated that while the 5’ end is required for target identification, the 3’ end is responsible for modulating repression strength [8, 9, 13]. Specifically, additional binding in the 3’ end of miRNAs with emphasis on nucleotides 12–17 demonstrated augmented miRNAs repression . Other structural features have also been accredited with facilitating miRNAs efficacy. For example, AU richness proximally flanking the seed region binding site has been shown to contribute to miRNAs efficacy, although these contributions quickly diminish with distance from the binding site [8, 14, 15]. In addition, the location of the miRNAs binding site within the 3’ UTR of mRNAs also plays a role in determining miRNAs functionality. Sites situated near either end of the 3’UTR of mRNAs have been reported to demonstrate greater activity compared to sites that reside in the center of the 3’UTR region of mRNAs. It should be noted that this effect was more pronounced in longer 3’UTRs and that site-conservation in these areas is greater than centrally located sequences. However, sites located to close the open reading frame or within 15 nucleotides of the stop codon have been found to have low binding affinity [8, 11, 16, 17]. Aside from these structural features, it has also been revealed that proximal miRNAs binding sites tend to interact synergistically while multiple sites at distance behave independently with an additive affect [8, 9, 13, 18, 19]. This synergistic affect potentially allows for efficient protein regulation with minimal increase in miRNAs cellular levels.
Meanwhile, sedimentation studies conducted with mRNAs under miRNAs repression yield results which indicate the association of polysomes. This provides evidence that particular miRNAs function through post-initiation translational interference [48, 49, 55–58]. Furthermore, the activity of the associated polysomes in translation was verified through puromycin-sensitivity assays . It can be postulated that two mechanisms may be involved in post-initiation suppression. First, miRNAs association could result in elongation interference leading to a dramatic reduction in elongation rate. Second, the presence of miRNAs may lead to premature ribosomal dissociation prior to the completion of translation as a consequence of mRNAs instability or physical impediment. Currently the exact mechanism is unclear, which may indicate the possibility that different miRNAs subtypes exist. Whether initial or post-initial translation repression is utilized, both would account for reductions in protein levels amidst unwavering mRNAs expression witnessed experimentally.
The recent discovery of a class of evolutionarily-conserved receptors has led to the re-examination of the signaling and function of the innate immune system. TLRs are a class of membrane-bound pattern recognition receptors that are capable of identifying particular pathogen associated molecular patterns (PAMPs) and danger associated molecular patterns (DAMPs). Initially discovered in insects, the Toll receptor was determined to play a function in innate immune protection against fungal infections. Pursuit of Toll homologues in humans revealed 11 members, which can identify a wide range of ligands. As Type I integral membrane glycoproteins, TLRs share significant cytoplasmic region homology with interleukin-1 receptors (IL-1R). Within the cytoplasmic tails of the TLRs/IL-1R is a highly conserved region of approximately 200 amino acids referred to as the Toll/IL-1R (TIR) domain . This conserved sequence contains three boxes - box 1 (FDAFISY), box 2 (GYKLC-RD-PG), and box 3 (a conserved W surrounded by basic amino acid residues). Boxes 1 and 2 are believed to be involved in the binding of signaling proteins, whereas box 3 is primarily thought to be involved in the localization of the receptor . Due to this homologous TIR domain, both TLR/IL-1 receptors utilize a shared downstream signaling pathway, which will be discussed in detail later. However, unique extracellular domains allow TLRs/IL-1 receptors to recognize different ligands. The extracellular regions of TLRs contain leucine-rich repeats which form a horseshoe shape and share no homology with IL-1R. Despite the conservation of this region among TLRs, individual receptors maintain the capability of recognizing very different ligands . TLR4 has been attributed with the recognition of several factors including fibrinogen, mouse mammary-tumor virus envelope proteins, taxol, respiratory syncytial virus fusion proteins, heat shock proteins, and lipopolysaccharide (LPS) while TLR3 can identify double-stranded viral DNA [61–68]. The location of ligands recognized by each TLR dictates whether the receptor is located on the cellular membrane or intracellularly. For example, TLR4 ligands are located extracellularly and therefore this particular receptor is found on the cell membrane. In contrast, TLRs −3, -7, and −9 which recognize double-stranded viral DNA, single-stranded RNA, and abnormal nucleic acid motifs respectively, are logically located intracellularly [69–71].
The cytokine interleukin-1 (IL-1) was first discovered in the early 1940s. Produced by active leukocytes and found to induce fever, the protein was aptly named “pyrexin” or “endogenous pyrogen” . It later became evident that this protein did more than induce fever as it mediated an array of biological activities and played a key role in the inflammatory process. Biologically, IL-1 is a potent inducer of chemokines, other cytokines, adhesion molecules, and inflammatory proteins. It soon became evident that the effects of IL-1 were actually the cumulative action of two cytokines, IL-1α and IL-1β [3, 4]. Although transcribed from two unique genes found adjacent on chromosome 2, IL-1α and IL-1β share a modest amino acid sequence homology of only 27% [5–8]. Despite this, both have a comparable mature three-dimensional structure [7, 8]. This similarity allows both IL-1α and IL-1β to bind to the same receptor, leading to the activation of shared downstream mediators . The 80 kDa receptor, IL-1R1, contains three extracellular immunoglobulin domains and an intracellular domain [31–33]. As mentioned previously, the intracellular portion of IL-1R1 contains a TIR domain and is the reason why it is grouped in the interleukin-1 receptor/Toll-like receptor superfamily. IL-1R1 interaction with IL-1 results in the recruitment of a second receptor chain termed IL-1R accessory protein (IL-1RAcP) . Dimerization of IL-1R with IL-1RAcP results in receptor activation.
MyD88-dependent signaling pathway
Containing six family members, the TNF receptor associated factors (TRAFs) are a group of evolutionary conserved adaptor molecules . Structurally, TRAFs contain a characteristic coiled-coil domain within the N-terminus while the C-terminus functions as an interface for upstream interactions and self-association . Functioning downstream of the IRAKs is TRAF6 which contains the binding motif P-X-E-X-X that is also found three times within IRAK1. TRAF6 binding to IRAK1 leads to the dissociation of TRAF6, IRAK1, and IRAK4 . This complex proceeds to the plasma membrane where it interacts with the complex of transforming growth factor-β (TGF-β)–activated kinase 1 (TAK1), TGF-β-activated protein kinase 1-binding protein 1 (TAB1), and TGF-β-activated protein kinase 1-binding protein 2 (TAB2). The mitogen-activated protein kinase (MAPK) kinase kinase TAK1 is essential in IL-1 and TNF-α-induced NF-κB activation . TAB1 is capable of boosting the activity of TAK1 while TAB2 acts as a facilitator of TAK1 activation by acting as an adaptor to TAK1/TRAF6 association [88, 89]. It should be noted that a TAB2 deficiency does not result in impaired IL-1 and TNF-induced NF-κB activation . This is most likely explained by the discovery of TAB3, which is believed to have TAB2 redundant activity. This hypothesis was validated when transfection with small interference RNA directed against both TAB2 and TAB3 resulted in abrogation of NF-κB activation induced by IL-1 and TNF-α . Once activated, TAK1 proceeds to interface with the inhibitor of NF-κB (IκB) kinase complex (IKK). This interaction leads to IKK activation causing downstream phosphorylation of IκBs resulting in their degradation. As a result, this frees NF-κB, a protein comprised of two subunits p50 and p65, from IκB repression. This allows NF-κB to translocate to nucleus where the transcription augmentation of NF-κB-dependent pro-inflammatory genes occurs.
Aside from NF-κB activation induced by the TLR/IL-1 signaling just discussed, an alternative pathway can be triggered midway through the signaling cascade by both TLRs and IL-1R. This results in the activation of p38 mitogen-activated kinases (p38), c-Jun N-terminal kinases (JNKs), extracellular signal-regulated protein kinases (ERKs), and the two transcription factors - activator protein-1 (AP-1) and activating transcription factor (ATF) [92–94]. While the exact signaling cascade which leads to the activation of these factors has yet to be clearly defined, it is believed that following TRAF6-induced activation, TAK1 associates with apoptosis signal-regulating kinase 1 (ASK1/MAPKKK5) under certain circumstances instead of IKK as mentioned above . In fact, it has been reported that overexpressed ASK1 can inhibit IL-1 induced NF-κB activation by competing with TRAF6 for TAK1 . Moreover, ASK1-deficient mice were shown to be protected from LPS-induced septic shock, demonstrating the significance of the alternative pathway in innate immune responses . Activation of ASK1 leads to the triggering of p38 and JNK signaling pathways . It should be noted that ASK1-deficient mice only display impaired signaling to LPS and not to the TLR-3 and −9 ligands poly(I:C) or CpG-DNA. Furthermore, impaired TLR-4 signaling appeared to be related to p38 signaling and not JNK signaling . Taken together this may indicate TLR specificity or TLR-specific MAPKKK in p38 and JNK signaling.
MyD88-independent signaling pathway
Although the MyD88-independent signaling pathway is less elucidated than the MyD88-dependent pathway, some key players have been identified. Required for interfacing with all TLRs is the presence of a TIR-domain within the direct downstream adaptor protein. To identify this protein, researchers relied on bioinformatics which led to the discovery of TIR-domain-containing adaptor protein inducing IFN-β (TRIF). It was quickly established that TRIF mediates the MyD88-independent pathway based on experimental results in TRIF-deficient mice which displayed impaired IFN-β production (IRF3) upon stimulation with TLR-3 and −4 ligands . Signaling downstream of TRIF are two non-canonical IKKs, IKKε and TBK1. Both have been shown to activate NF-κB and IRF-3 [100–105].
Another TIR-domain containing protein, TRIF-related adaptor molecule (TRAM), was also identified by in silico studies . Again, knockout mice were utilized to identify the role of TRAM in TLR/IL-1 signaling. Experimentation with IL-1β revealed no impairment of NF-κB activation, JNK activation, or IL-6 production in TRAM knockouts. This result indicated that TRAM was not involved in the shared TLR/IL-1 MyD88-dependent signaling pathway. This conclusion was further supported when TRAM-deficient mice displayed normal immunological responses to ligands for TLR-2, -7, and −9, which all exclusively use the canonical pathway. Interestingly, exploration of the role of TRAM in MyD88-independent signaling revealed that TLR-4 but not TLR-3 production of IFN-β or other IFN-inducible genes were impaired in TRAM-deficient mice . This suggests an exclusivity of TRAM in TLR-4-mediated MyD88-independent signaling. Currently, it is believed that TRAM facilitates TRIF binding with TLR-4 in a similar fashion as MAL in MyD88/TLR-4 association.
Further examination in TRAM knockout mice revealed that TLR-4-mediated NF-κB activation was impaired in a time-dependent fashion. Initial NF-κB activation, 10–20 minutes following LPS treatment, was undeterred while levels were diminished at 60–120 minutes when compared with wild-type controls. Similarly, JNK activity was also found to be limited in a time-dependent manner . These findings indicate a divergent role between MyD88-dependent and –independent signaling pathways. MyD88-dependent signaling induces a rapid immunological response while MyD88-independent signaling triggers a delayed response allowing for immune sustainability. To ensure that the MyD88-dependent and –independent are the only pathways utilized by TLR-4, MyD88/TRIF double knockout mice were bred. In this model LPS-induced NF-κB activation was completely abolished suggesting the absence of any further signaling cascades.
IL-6, a known proinflammatory cytokine, is produced in response to challenge with several TLR ligands and IL-1 signaling. However, the exact signaling required for this induction has not been resolved; specifically, the upstream signal transduction. It has been reported that the NF-κB subunit, p50, can increase IL-6 expression. Furthermore, signal transducer and activator of transcription 5 (STAT5) is activated by LPS treatment in mouse monocyte/macrophage RAW cells and peritoneal macrophages. A possible interaction between p50 and STAT5 was examined and confirmed with co-immunoprecipitation assay in African green monkey kidney fibroblast-like COS-7 cells. To determine the mechanism of IL-6 induction, a chromatin immunoprecipitation assay was conducted and showed that both p50 and STAT5 located to the promoter of IL-6. Traditionally, functioning upstream of STATs are Janus kinases (JAKs). In particular, JAK2 expression was found to be augmented within 1–5 minutes following LPS treatment in RAW cells. This finding, along with comparative downstream effects following deletion of either JAK2 or STAT5, indicates that both are involved in this signaling pathway. Investigation of further upstream signaling revealed that JAK2 could not associate individually with either MyD88 or TLR-4 in co-immunoprecipitation assays. However, JAK2 was found to interact with the MyD88/TLR-4 complex. This evidence suggests the existence of an unidentified adaptor molecule that facilitates this interaction. Interestingly, IL-6 has been demonstrated to induce suppressor of cytokine signaling 1 (SOCS1), a known negative regulator of cytokine signaling. The role of SOCS in TLR/IL-1 signaling will be discussed in-depth in the following section .
Endogenous TLR/IL-1 signaling regulators
Hyper-activation of immune signaling pathways can seriously impact overall health, leading to a wide variety of chronic inflammatory and autoimmune illnesses. Each adaptor molecule within the TLR/IL-1 signaling pathway is under stringent control via phosphorylation, physical interactions, conformational changes, as well as ubiquitin and proteasomal degradation. Furthermore, there are regulatory proteins designed to attenuate TLR/IL-1 signaling which will now be discussed.
A family of eight proteins aptly referred to as the suppressors of cytokine signaling (SOCS), primarily function to inhibit cytokine signaling. Specifically, SOCS1 is induced by LPS and CpG-containing DNA, known activators of the TLR/IL-1 signaling . This led researchers to investigate whether SOCS1 played a role in mitigating TLR/IL-1 signaling. Further investigation revealed that SOCS1-deficient mice were hypersensitive to LPS challenge [109, 110]. In addition, treatment of SOCS1-deficient mice with CpG DNA and macrophage-activating lipopeptide 2 both resulted in the augmented expression of pro-inflammatory cytokines [109–113]. These findings led investigators to screen for specific SOCS1 targets within the canonical TLR/IL-1 pathway. SOCS1 was found to directly target the p65 subunit of NF-κB leading to its ubiquitination and degradation . It was also found to target and mitigate the activity of MAL resulting in the diminished down-stream activation of NF-κB . Aside from regulating the canonical TLR/IL-1 pathway, it has also been reported that SOCS1 regulates the alternative MyD88-dependent pathway by suppressing ASK1. SOCS1 can also alleviate TLR MyD88-independent signaling as well. As discussed earlier, the activation of MyD88-indepenedent signaling pathway results in the up-regulation of IFN-β. It has been shown that SOCS1 represses downstream IFN-β effects on JAK/STAT signaling . In addition, the JAK2/STAT5/IL-6 signaling pathway has also been shown to be repressed by SOCS1 . The ability of SOCS1 to mitigate the activity of the canonical TLR/IL-1 MyD88-dependent signaling pathway, the alternative MyD88-dependent pathway, downstream MyD88-independent signaling, and IL-6 production indicates the significance of SOCS1 in mediated the innate immune system.
SH2-containing inositol phosphatase, SHIP1, is a protein tyrosine phosphatase and a potent endogenous NF-κB inhibitor. SHIP1 has been shown to negatively regulate several tyrosine kinase-dependent signaling pathways in myeloid cells, B-lymphocytes, and T-lymphocytes . The expression of SHIP1 and its phosphorylation is induced by LPS. This led researchers to evaluate the role of SHIP1 in TLR/IL-1 signaling and the discovery that SHIP1 negatively regulates this signaling [118–120]. In fact, this finding may explain the systemic autoimmunity and severe inflammation observed in SHIP1-deficient mice. In macrophages, TNF-α and IL-6 production was diminished after LPS stimulation with SHIP1 over-expression . This observation was supported by the data gathered from SHIP1 RNA-interfering studies. In addition, SHIP1 was also found to inhibit the activation of MAPKs induced by LPS stimulation.
The immunosuppressive/anti-inflammatory interleukin, IL-10, has also been attributed with mitigating TLR/IL-1 MyD88-dependent signaling but not MyD88-independent signaling. Levels of IL-6 were found to be augmented in the presence of an IL-10R antagonist and diminished with IL-10 treatment following exposure to MyD88-dependent signaling activator CpG-containing DNA. This effect was not seen when treated with PolyI:C, a MyD88-independent signaling ligand. The diminished MyD88-dependent signaling associated with the treatment of IL-10 can be attributed to diminished protein levels of IRAK4 and TRAF6. In fact, IL-10 antagonism led to prolonged protein levels of IRAK4 and TRAF6 following LPS treatment in comparison to controls. This observation appears to be the direct result of increased ubiquitination of IRAK1, IRAK4, and TRAF6 seen with the treatment IL-10. In support of these findings, the effects of IL-10 on MyD88-dependent signaling were abolished with proteasomal inhibition . It should also be noted that the expression of IL-10 can be regulated by the pro-inflammatory protein programmed cell death 4 (PDCD4). In fact, the inhibition of PDCD4 with siRNA resulted in elevated levels of IL-10 following LPS stimulation. Similar observations were also made in PDCD4-deficient mice.
miRNAs regulation of TLR/IL-1β signaling
Crucial for proper immune system function, TLR/IL-1 signaling is under stringent regulation. This adept regulation is required to ensure proper host defense while mitigating the deleterious effects of the inflammation process. Previously discussed regulators like SOCS1 and SHIP1 can inhibit various aspects of this shared pathway via transcription and post-translational mechanisms, and we will now discuss the fine-tuned regulation of the TLR/IL-1 pathway by miRNAs via miRNAs-directed mRNAs translation repression or cleavage [6, 7].
miRNAs modulation of TLRs/IL-1R
miRNAs Targets within the TLR/IL-β Signaling Pathway
TLR/IL-1β Signaling Molecules
miRNAs regulation of TLR/IL-1β adaptor molecules
Despite the limited number of experimentally verified miRNAs that interact with TLR/IL-1β receptors, there are abundant instances of miRNAs targeting TLR/IL-1 adaptor molecules. This encompasses miRNAs that target multiple adaptors within the TLR/IL-1 pathway and those that target individual proteins. As discussed earlier, MyD88 is an integral adaptor molecule in proper TLR/IL-1 signaling, functioning as the signal transducer for IL-1R and all TLRs, except TLR-3. Therefore, it should not be surprising that this prominent signaling protein is targeted by miRNAs. Predicted by TargetScan and experimentally verified with luciferase reporter assays, it has been clearly demonstrated that miR-155 directly regulates the expression of MyD88. Additionally, the transfection of cells with miR-155 mimics revealed that MyD88 protein expression was modulated in a time and dose-dependent manner. To determine the method of miRNAs repression, RT-PCR was performed following mimic treatment. This resulted in no change in MyD88 mRNAs levels, suggesting that miR-155 modulates MyD88 protein expression by mRNAs translation repression rather than mRNAs degradation . The adaptor protein MAL, which facilitates MyD88 interaction with TLR-2 and TLR-4, has also been found to be regulated by miRNAs. Predicted to be targeted by miR-145, this interaction was confirmed by luciferase reporter assays in human embryonic kidney 293 (HEK293) cells. Western blots were then carried out following mimic or inhibitor transfection of bone marrow cells which verified that MAL protein expression was diminished and augmented, respectively. Unfortunately, mRNAs levels were not examined so the exact mechanism of miRNAs repression cannot be cited. Acting downstream and associating with MyD88, IRAK1 is essential to TLR/IL-1 signaling and has been identified as a target of miR-146a/b. Again, this association was predicted utilizing online software and then confirmed with luciferase reporter assays . This interaction was further confirmed by another group with the transfection of miR-146a mimics and inhibitors in macrophages . IRAK4 has yet to have miRNAs association experimentally identified. This may be the result of the fact that it plays a role in, but is not essential to, TLR signaling. Despite this, the possibility of miRNAs interactions should not be ruled out until further investigation is concluded. Functioning downstream of the IRAKs, TRAF6 has been confirmed to be targeted by miR-146. Luciferase reporter assays with TRAF6 3’UTR-containing vectors verified this association. Online prediction algorithms were then used to identify the exact binding site so that a mutated vector could be constructed. The use of this mutant in luciferase reporter assays confirmed binding site specificity. Mitigation of TRAF6 protein expression was then verified by Western blot following mimic and inhibitor transfection [126, 127]. Downstream of TRAF6 is TAK1 which functions to activate the IKK complex. The prediction of a conserved miRNA-10a binding site by TargetScan within the 3’UTR of TAK1 was confirmed in HEK293 cells . Furthermore, it was experimentally demonstrated that TAK1 mRNAs and protein was elevated with miR-10a knockdown in human aortic endothelial cells by qRT-PCR and Western blot, respectively. Facilitating the protein-protein interaction of TRAF6 and TAK1 is TAB2. This important adaptor has been identified to be modulated by miR-155. A predicted association by TargetScan, the miR-155:TAB2 interaction was proven through Western blot, qRT-PCR, and luciferase reporter assay results. This repression was supported by the similar findings by another independent research group . The IKK complex is located downstream of TAK1 and contains 3 subunits. Two of those subunits, IKK-α and IKK-β, have been found to be under miRNAs regulation. IKK-α is mediated by miR-223, miR-15a, and miR-16 while IKK-β is targeted by miR-199. The association between IKK-α and all three miRNAs were confirmed individually with luciferase reporter assays by creating constructs mutated for each specific miRNAs binding site. In addition, transfection with mimics for all three miRNAs results in diminished Iκk-α protein levels in human cervical cancer (HeLa) cells and macrophages. As predicted by PicTar, IKK-β contains three putative miR-199 binding sites. A luciferase reporter assay was utilized to confirm this targeting while the use of mimics and inhibitors demonstrated that IKK-β protein levels could be mitigated. Once the IKK complex phosphorylates IκB, NF-κB is freed from repression to translocate to the nucleus. As mentioned earlier, NF-kB contains two subunits p50 and p65. NFκB1 mRNAs, which is cleaved to form the mature NF-κB subunit p50, has been found to be directly modulated by miR-9. This subunit is significant in the transactivation of the p65 subunit allowing NF-κB to translocate to the nucleus. The direct interaction between miR-9 and NFκB1 was again established with luciferase reporter assays while overexpression of miR-9 in monocytes resulted in diminished NFκB1 protein levels. Unique to the MyD88-independent pathway, IKKε has also been identified as a target of miR-155. Predicted by the prediction algorithms, TargetScan and RNAhybrid, direct association was experimentally confirmed by luciferase reporter assays. Revealed by qRT-PCR, the mRNAs levels of IKKε were found to be altered by the transfection of human gastric epithelial GES-1 cells with miR-155 mimics. This indicates that miR-155 alters IKKε protein expression by mRNAs cleavage.
miRNAs mitigation of TLR/IL-1 signaling regulators
The activity of signaling regulators must be closely monitored to ensure adequate activity and suppression of the TLR/IL-1 cascade when necessary. Therefore, the critical nature of these regulatory proteins perpetuates their potential for modulation by miRNAs. As mentioned previously, SOCS1 plays an integral role in regulating TLR/IL-1 signaling by targeting several proteins within the pathway. The ability of miRNAs to modulate SOCS1 expression would therefore have a significant effect on TLR/IL-1 signaling. In fact, several groups have reported that SOCS1 expression can be directly mediated by miR-155 [130–132]. Direct association between SOCS1 mRNAs and miR-155 was confirmed using luciferase reporter constructs containing the native 3’ UTR of SOCS1 mRNAs. Using the target prediction software PicTar, the miR-155 binding site sequence was identified and the native 3’ UTR of SOCS1 mRNAs was mutated to verify binding-site specificity. Also, an arbitrary miRNA not predicted to bind to SOCS1 was tested to verify miR-155 specificity [130, 131]. This direct association was then further verified with a gel shift mobility assay that examined the ability of miR-155 to form duplexes with the predicted binding site sequence within the native 3’ UTR of SOCS1 mRNAs. Again, a mutated sequence was used to verify the binding site while anti-sense miR-155 was utilized as a positive control . With association clearly demonstrated by the previous experiments, Western blot analysis was performed to verify that SOCS1 protein expression was mitigated. Transfection with miR-155 mimics or with miR-155-containing adenoviruses both impaired the protein expression of SOCS1 in a similar fashion to SOCS1 siRNAs [131, 132]. These results were further supported by observations in miR-155 deficient mice, which showed elevated SOCS1 expression in T-cells . Notably, SOCS1 mRNAs levels remained unaffected in the presence of miR-155 mimics, miR-155 inhibitors, or in miR-155-deficient mice. This indicates that SOCS1 expression is modulated by miR-155 via translational repression but not via mRNAs degradation.
miR-155 not only plays a critical role in SOCS1 expression but also has been confirmed in epithelial cells, macrophages, and myeloid cells to regulate SHIP1 expression [133–135]. Bioinformatics exploration with the target prediction software TargetScan revealed a highly conserved miR-155 binding site in SHIP1’s 3’UTR. In fact, this was the only highly conserved miRNAs binding site within the 3’UTR of SHIP1 . Direct association was experimentally confirmed with a luciferase report assay where 293 T-cells were co-transfected with miR-155 mimics and wild-type SHIP1 3’UTR reporter plasmids. Those cells transfected with the mimics displayed impaired chemoluminescence in comparison to those transfected with the control miRNAs [134, 135]. Predictably, cells transfected with scrambled SHIP1 3’UTR reporter plasmids showed no variability in chemoluminescence with either miR-155 mimics or controls [133, 134]. In a separate study where cystic fibrosis IB3 epithelial cells have diminished levels of SHIP1 mRNAs compared to normal IB3 epithelial cells, treatment of fibrosis IB3 epithelial cells with miR-155 inhibitors led to increased luciferase activity associated with normal IB3 SHIP1 levels . With miR-155:SHIP1 association confirmed, Western blots were performed to verify a change in SHIP1 protein levels. Utilizing miR-155-containing vectors it was demonstrated that SHIP1 expression was reduced 2.3 folds. Additionally, the use miR-155 mutant vectors showed no significant fold changes, verifying miR-155 specificity . Furthermore, observation of miR-155-deficient macrophages revealed elevated SHIP-1 protein expression in comparison with wild-type controls [133, 134]. RAW 264.7 cells transfected with wild-type miR-155 vectors led to an approximately 50% reduction in SHIP1 mRNAs when compared to the flag control. This effect was not seen in transfections with miR-155 mutant vectors . These findings were supported by the data collected from a separate investigator conducting transfections with miR-155 mimics and inhibitors. Again, the expression of SHIP1 mRNAs was diminished by approximately 50% with miR-155 overexpression while inhibition of miR-155 function led to an equivalent increase . Taken together, these dramatic changes in SHIP1 mRNAs indicate that miRNA-155 regulates SHIP1 expression by mRNAs degradation.
IL-10 is a prototypical anti-inflammatory cytokine which has been shown to affect IRAK4 and TRAF6 ubiquitination as described earlier. Utilizing three miRNAs target prediction programs, miR-106a was identified as having a binding-site in IL-10’s 3’UTR. Experiments conducted in B lymphoma lymphoblast-like Raji cells with miR-106a mimics resulted in a dose-dependent reduction of IL-10 protein in the culture supernatant. IL-10 mRNAs levels were also reduced in a dose-dependent manner when examined by qRT-PCR. The target site within the 3’UTR of IL-10 mRNAs was then confirmed in T cell leukemia Jurkat cells and Raji cells with luciferase reporter assays. These results indicate that IL-10 expression is capable of being transcriptionally regulated by miR-106a . Mentioned previously, pro-inflammatory protein PDCD4 is capable of repressing IL-10 and therefore the repression of PDCD4 by miRNAs would in effect increase IL-10’s TLR/IL-1 regulatory activity. As it is, miR-21 has been found to target PDCD4. Transfecting RAW264.7 cells with miR-21 inhibitors mitigated the inhibitory effects of PDCD4 on IL-10 typically seen following LPS stimulation. Similar findings were found when competitive oligonucleotides that were specific to the miR-21 binding site in the PDCD4 3’UTR were used .
miRNAs expression modulation by TLR/IL-1 signaling
As we have discussed, several miRNAs play an important role in regulating the TLR/IL-1 signaling pathway. Interestingly, recent findings have revealed that the activation and induction of this very important pathway can modulate the expression of several miRNAs . Among them are some of the same miRNAs that regulate TLR/IL-1 signaling, indicating that these miRNAs participate in either positive or negative feedback mechanisms. The first miRNA identified to be modulated by TLR/IL-1 signaling was miR-146. Its expression was shown to be rapidly augmented following the exposure of human acute monocytic leukemia THP-1 cells to LPS. Further studies have shown that miR-146 can also be induced by ligands for TLR-2, -3, and −5 in macrophages, bone marrow-derived monocytes, and T-cells . In addition, the expression of miR-146 was shown to be up-regulated by IL-1β . Mechanistically, the ability of TLR/IL-1 signaling to induce miR-146 expression has been shown to be NF-κB-dependent. Taken into account that experiments with over-expressing miR-146 epithelial cells and fibroblasts resulted in a reduction of IL-1-induced cytokine expression, and that miR-146 is known to target the critical TLR/IL-1 signaling molecules IRAK1 and TRAF6, one can conclude that miR-146 is capable of mitigating a TLR/IL-1 inflammatory response in a negative feedback manner.
One of the most extensively characterized miRNAs, miR-155, has been proven to be induced by several TLR ligands in mouse bone marrow-derived macrophages. Specifically, ligands for TLR-2, -3, -4, and −9, were found to augment miR-155 expression . Interestingly, this indicates that both the MyD88-dependent and MyD88-independent pathway can augment miR-155 expression. Mechanistically, both TLR/IL-1 downstream transcription factors, NF-κB and AP-1, have been shown to facilitate the transcription activation of the B-cell integration cluster (Bic), the gene that encodes for miRNA-155 [140, 141]. This data clearly shows that miR-155 expression is augmented by TLR/IL-1 activity while direct miR-155 targeting of TLR/IL-1 signaling molecules MyD88, TAB2, and IKKε suggests that miR-155 can suppress TLR/IL-1 signaling by a negative feedback mechanism. Interestingly, miRNA-155 has also been credited with reciprocally facilitating TLR/IL-1 signaling. Experimentally, over-expression of miR-155 in the bone marrow compartment of adult mice led to myeloproliferation typically observed following LPS injection . This may be explained by the fact that miR-155 is known to directly repress the activities of TLR/IL-1 signaling inhibitors SHIP1 and SOCS1. In this case, miR-155 would participate in a positive-feedback loop.
These differing results suggest several key points about miRNAs regulation. First, miRNAs activity and expression is heavily dependent on specific cell types; second, the preferential targeting of individual mRNA may be highly dependent on the expression of those mRNA or other unforeseen factors; and third, that seeming contradictive targeting may in fact be avoided by precisely orchestrated timing. Taking these considerations into account the targeting of SOCS1 and SHIP1 by miRNA-155 may initially be required to augment novel TLR/IL-1 signal transduction when an immune response is required. However, the perpetuation of this signal may need to be suppressed after a specific duration of inflammation initiation to prevent detrimental effects. Under these conditions the repression of adaptor molecules MyD88, TAB2, and IKKε would be required.
miR-9 has also been shown to be augmented by TLR/IL-1 activity in monocytes and granulocytes [143, 144]. While mature miR-9 can be produced from three unique primary transcripts encoded by three distinct genes, LPS treatment only induced one of these primary transcripts, miR9-1. The promoter region of the chromosome 1 open reading frame 61 (C1orf61) locus, which generates this particular primary transcript, contains consensus binding sites for known LPS-sensitive transcription factors like NF-κB. This evidence suggests that rapid activation of the TLR/IL-1 signaling pathway leads to the induction of miR-9 through NF-κB-directed transcription of the miR9-1 transcript. In fact, the necessity of NF-κB in miR-9 induction was confirmed with the use of several different NF-κB inhibitors. Furthermore, inhibitors for p38 and JNK demonstrated no inhibitory effect on miR-9 induction following TLR/IL-1 signaling activation, further suggesting NF-κB’s involvement. Therefore, miR-9 can be described as a negative feedback regulator of TLR/IL-1 signaling since it targets the NF-κB subunit 50 as previously mentioned .
Another miRNAs shown to be elevated by TLR/IL-1 signaling in B-cells, cholangiocytes (epithelial cells of the bile duct), macrophages, and inflamed lung tissue is miR-21 [137, 145–147]. This effect was abolished in immortalized, bone-marrow derived monocytes (BMDMs) deficient in MyD88 but was only slightly diminished in TRIF-deficient BMDMs. This suggests that the augmentation of miR-21’s expression is NF-κB-dependent. Further investigation revealed that the promoter of miRNA-21 contains a predicted NF-κB binding site. Its significance was confirmed in mouse embryonic fibroblasts that were deficient in the NF-κB p65 subunit. In these cells miRNA-21 levels could not be induced by LPS. Following LPS stimulation in RAW264.7 macrophages, the levels of miR-21 are not significantly induced until 4 hours and continue to increase until 24 hours. This observation is in accordance with the fact that PDCD4 expression immediately increases following LPS treatment but then is diminished at 4 hours and abolished at 24 hours . Clearly, the expression of PDCD4 following LPS treatment is dependent on miR-21 expression. Furthermore, the reduction of PDCD4 by miR-21 explains the augmented levels of IL-10 observed after LPS treatment. The induced expression of miR-21 by TLR/IL-1 signaling results in a negative feedback loop by reducing PDCD4, causing greater IL-10 activity.
The TLR/IL-1 signaling pathway is critical to proper immune functioning. Hyper-activation has been associated with several autoimmune and chronic pathologies while hypo-activation leaves the host highly susceptible to infection. Therefore, it is not surprising that this signaling pathway is under stringent control by phosphorylation, physical interactions, conformational changes, regulatory proteins, as well as ubiquitin and proteasomal degradation. We have also discussed the activity of miRNAs in further modulating this signaling system. Interestingly, many of these exact miRNAs are also induced by the activation of the TLR/IL-1 signaling cascade, indicating a mutual regulation between miRNAs and TLR/IL-1 pathway via a complex positive- and negative-feedback loop system . The role of these participating miRNAs needs to be closely evaluated as they hold vast potential as novel therapeutics.
This work was partially supported by the National Institutes of Health Grants HL094451 and HL108910 (XFY), HL67033, HL82774 and HL77288 (HW) and American Heart Association Fellowship 11640013 (AV).
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