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SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines
© Quentmeier et al; licensee BioMed Central Ltd. 2009
Received: 25 November 2008
Accepted: 23 January 2009
Published: 23 January 2009
SET-NUP214 fusion resulting from a recurrent cryptic deletion, del(9)(q34.11q34.13) has recently been described in T-cell acute lymphoblastic leukemia (T-ALL) and in one case of acute myeloid leukemia (AML). The fusion protein appears to promote elevated expression of HOXA cluster genes in T-ALL and may contribute to the pathogenesis of the disease. We screened a panel of ALL and AML cell lines for SET-NUP214 expression to find model systems that might help to elucidate the cellular function of this fusion gene.
Of 141 human leukemia/lymphoma cell lines tested, only the T-ALL cell line LOUCY and the AML cell line MEGAL expressed the SET(TAF- Iβ)-NUP214 fusion gene transcript. RT-PCR analysis specifically recognizing the alternative first exons of the two TAF- I isoforms revealed that the cell lines also expressed TAF- Iα-NUP214 mRNA. Results of fluorescence in situ hybridization (FISH) and array-based copy number analysis were both consistent with del(9)(q34.11q34.13) as described. Quantitative genomic PCR also confirmed loss of genomic material between SET and NUP214 in both cell lines. Genomic sequencing localized the breakpoints of the SET gene to regions downstream of the stop codon and to NUP214 intron 17/18 in both LOUCY and MEGAL cells. Both cell lines expressed the 140 kDa SET-NUP214 fusion protein.
Cell lines LOUCY and MEGAL express the recently described SET-NUP214 fusion gene. Of special note is that the formation of the SET exon 7/NUP214 exon 18 gene transcript requires alternative splicing as the SET breakpoint is located downstream of the stop codon in exon 8. The cell lines are promising model systems for SET-NUP214 studies and should facilitate investigating cellular functions of the the SET-NUP214 protein.
Leukemia subtypes are often associated with specific recurrent chromosome translocations. Translocations may function by constitutively activating proto-oncogenes or they may create new oncogenes by fusing two formerly independent genes. The SET-NUP214 (TAF-1/CAN) gene fusion has previously been described as result of a chromosomal translocation t(9;9)(q34;q34) in a case of acute undifferentiated leukemia . The fusion gene appears to inhibit differentiation, while secondary chromosomal aberrations are necessary to induce tumorigenesis [2, 3]. Recent studies have shown that the SET-NUP214 fusion can also result from a recurrent deletion, del(9)(q34.11q34.13) in patients with T-cell acute lymphoblastic leukemia (T-ALL) . It has also been reported in a single case of acute myeloid leukemia (AML) . SET-NUP214 positive T-ALL patients exhibited high expression levels of HOXA cluster genes . Downregulation of the fusion gene repressed HOX gene expression and induced differentiation in the SET-NUP214 positive cells confirming that SET-NUP214 keeps hematopoetic cells in an undifferentiated stage .
We screened a panel of 141 human cell lines to investigate the occurrence of the SET-NUP214 fusion in different hematologic malignant contexts.
Results and discussion
HOXA cluster genes are described as targets of the SET-NUP214 fusion protein . Accordingly, downregulation of SET-NUP214 expression decreases HOX gene expression and inhibits proliferation in the SET-NUP214 positive T-ALL cell line LOUCY . We performed quantitative RT-PCR to verify whether cell lines with high expression levels of SET-NUP214 also expressed above average levels of HOXA9. Confirming a positive correlation between SET-NUP214 and HOX gene expression, quantitative real-time PCR revealed more than 1000× higher HOXA9 levels in the SET-NUP214 positive cell line LOUCY than in six other T-ALL cell lines tested (data not shown). HOXA9 expression levels were also high in cell line MEGAL, but not above many SET-NUP214 negative AML cell lines (data not shown) which may be due to the fact that HOXA cluster genes are often highly expressed in myeloid leukemias [7, 8].
We demonstrated the presence of the SET-NUP214 gene in the T-ALL cell line LOUCY and in the AML cell line MEGAL by genomic sequencing. In both cell lines, the centromeric fusion is located downstream to the stop codon of SET. Therefore, alternative splicing might turn out to be obligatory for expression of SET-NUP214 mRNA.
Human cell lines
The 141 continuous cell lines investigated in this study were either taken from the stock of the cell bank (DSMZ – German Collection of Microorganisms and Cell Cultures) or were generously provided by the original investigators. Detailed references and cultivation protocols have been described previously .
SET-NUP214 screening and breakpoint determination
Screening of cell lines for SET/NUP214 mRNA expression was performed applying RT-PCR. RNA was prepared using the Trizol reagent (Invitrogen, Karlsruhe, Germany). For mRNA quantification, reverse transcription was performed using the SuperScript II reverse transcriptase kit (Invitrogen, Karsruhe, Germany). Previous studies identified SET exon 7/NUP214 exon 17 and SET exon 7/NUP214 exon 18 fusions in T-ALL and AML patients [4, 5, 10]. We applied primers from SET exon 6 and NUP214 exon 20 for SET-NUP214 expression screening. Analyses were repeated with previously described primers from SET exon 7 and NUP214 exon 18 : SET exon 6 forward: 5'-GAA GAG GCA GCA TGA GGA AC-3'; NUP214 exon 20 reverse: 5'-TAC TTT GGG CAA GGA TTT GG-3'; SET exon 7 forward: 5'-TGA CGA AGA AGG GGA TGA GGA T-3'; NUP214 exon 18 reverse: 5'-ATC ATT CAC ATC TTG GAC AGC A-3'. The same NUP214 exon 18 reverse primer was used in combination with alternative exon 1 forward primers to detect TAF-Iα-NUP214 and TAF-Iβ (SET)-NUP214 mRNA isoforms: TAF-Iα exon 1 forward: 5'-TAA ACG CCA GTC TCC ACT CC-3', TAF-Iβ (SET) exon 1 forward: 5'-AGC TCA ACT CCA ACC ACG AC-3'. For the determination of genomic SET and NUP214 breakpoints in cell lines LOUCY and MEGAL, genomic PCR was performed with the following sets of primers: (i) SET exon 7 forward: 5'-TGA CGA AGA AGG GGA TGA GGA T-3'; NUP214 exon 18 reverse: 5'-ATC ATT CAC ATC TTG GAC AGC A-3'. (ii) SET intron 8/exon 8 forward: 5'-TCA GGA GGA TGA AGG AGA AGA-3'; NUP214 intron 17/18 reverse: 5'-GAG GTG GCA GAG AGG TGG TA-3'; (iii) SET exon 8 forward: 5'-CTG CCA CTC AAT GGG AGA AT-3'; NUP214 intron 17/18 reverse: 5'-ACA AGA ATT ACC CGG GTG TG-3'; PCR was performed in a total volume of 50 μl with a DNA thermal cycler (Perkin Elmer Cetus, Heidelberg, Germany) for 35 cycles under standard conditions. Products were electrophoresed in 1.2% agarose gels and observed under UV light. PCR products were ligated into the pGEM-T Easy Vector System (Promega, Mannheim, Germany) and sequenced (Eurofins MWG Operon, Martinsried, Germany).
FISH was performed as described previously . Tilepath bacterial artificial chromosome (BAC) and fosmid clones were sourced from BAC-PAC Resources (Children's Hospital, Oakland, CA, USA). Probe preparation and labelling were as described previously . Imaging and analysis were performed using an Axioscope 2 fluorescence microscope system (Zeiss, Göttingen, Germany) and Cytovision software (Applied Imaging, Newcastle, UK).
Quantitative PCR analysis
Quantitative PCR was carried out using a 7500 Applied Biosystems real-time PCR system following the manufacturer's protocol (Darmstadt, Germany). TaqMan probes (Applied Biosystems) were used to quantify human HOXA9 (Hs00365956_m1) expression levels with TBP as endogenous control. For copy number analysis of genomic DNA, we performed relative quantitative PCR with the following oligonucleotides: ABL1 forward: 5'-CAC CGT TAA TTG GGA CTG TGT G-3'; ABL1 reverse: 5'-AAT GGT AGA GTG GTG CTC CTT G-3'; CRAT forward: 5'-CCT GTC CAG TTG GTC ACA CTC-3'; CRAT reverse: 5'-GCC TTT CTA GCT TGA TGC CTC-3'; NUP214 forward: 5'-GGC CAG GTT GGA TTT CAT AC-3'; NUP214 reverse: 5'-CTC ATG ATC CAG GGT GAC AG-3'; SET forward: 5'-TAG ACA GCG CCT AGC ACA TC-3'; SET reverse: 5'-TCC CTT CCA GTC CTG TTA ATG. PCR reactions were performed using SYBR-green chemistry under standard conditions. Values were calculated by the 2-ΔΔCt method. As endogenous control, the repetitive element LINE1 was used.
Western blot analysis
Analysis of SET-NUP214 protein expression was performed as follows: 1 × 106 cells were pelleted and washed with ice-cold phosphate-buffered saline (PBS), resuspended and boiled for 10 min in 25 μl SDS sample buffer containing 15% glycerol, 125 mM Tris-HCl pH 6.8, 5 mM EDTA, 2% SDS, 0.1% bromophenol blue and 1% β-mercaptoehanol. The samples were separated on 7% or 12% gels depending on the size of the wild-type proteins to be detected. Blotting and staining conditions were as described previously . The anti human SET Ab reacting with amino acids 3–18 was purchased from Abcam (Cambridge, UK), the anti human NUP214 Ab directed against the C-terminal part of the protein, was obtained form antibodies-online (Aachen, Germany).
We thank Professor Cristina Mecucci and Dr. Paolo Gorello (both from the Hematology and Bone Marrow Transplantation Unit, University of Perugia, Italy) for providing SET/NUP214 positive control cDNA.
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