Predominant cerebral cytokine release syndrome in CD19-directed chimeric antigen receptor-modified T cell therapy
Journal of Hematology & Oncology volume 9, Article number: 70 (2016)
Chimeric antigen receptor-modified (CAR) T cells targeting CD19 (CART19) have shown therapeutical activities in CD19+ malignancies. However, the etiological nature of neurologic complications remains a conundrum. In our study, the evidence of blood-brain barrier (BBB)-penetrating CAR T cells as a culprit was revealed. A patient with acute lymphocytic leukemia developed sustained pyrexia with tremors about 6 h after CART19 infusion, followed by a grade 2 cytokine release syndrome (CRS) and neurological symptoms in the next 3 days. Contrast-enhanced magnetic resonance showed signs of intracranial edema. Lumbar puncture on day 5 showed an over 400-mmH2O cerebrospinal pressure. The cerebrospinal fluid (CSF) contained 20 WBCs/μL with predominant CD3+ T cells. qPCR analysis for CAR constructs showed 3,032,265 copies/μg DNA in CSF and 988,747 copies/μg DNA in blood. Cytokine levels including IFN-γ and IL-6 in CSF were extremely higher than those in the serum. Methyprednisone was administrated and the symptoms relieved gradually. The predominance of CART19 in CSF and the huge discrepancies in cytokine distributions indicated the development of a cerebral CRS, presumably featured as CSF cytokines largely in situ produced by BBB-penetrating CAR T cells. For the first time, we reported the development of cerebral CRS triggered by BBB-penetrating CAR T cells.
Trial registration: ChiCTR-OCC-15007008.
Chimeric antigen receptor-modified (CAR) T cells targeting CD19 (CART19) have shown therapeutical activities in refractory/relapsed acute lymphocytic leukemia (ALL) [1–4]. Neurologic complications were reported in several trials [5–8]; however, the etiological nature still remains a conundrum. In our recent CART19 clinical trial (ChiCTR-OCC-15007008), the evidence of blood-brain barrier (BBB)-penetrating CAR T cells as a culprit was revealed.
An enrolled female patient of BCR/ABL p210(+) refractory/relapsed ALL with previous recurrence of central nervous system lymphoma (CNSL) was infused with autologous CART19 after conditioning chemotherapy with fludarabine and cyclophosphamide (detailed patient and methodological information was included in Fig. 1a and Additional file 1). CART19 preparation and infusion were described in Fig. 1b, c and Additional file 2 . About 6 h after CART19 infusion, the patient developed sustained pyrexia with tremors (Fig. 2a). Three days later, the patient complained of headache, vomit, and recurrent right-sided facial and limb paresis with a homolateral blurred vision and defective visual field. Physical examination showed decreased myodynamia (grade 3), high blood pressure, and positive Babinski and Kernig signs. Papilloedema was observed by ophthalmoscope. Contrast-enhanced magnetic resonance imaging showed signs of intracranial edema (Fig. 2b). Serum cytokines including INF-γ, IL-6, and IL-10 elevated synchronously, supporting a grade 2 cytokine release syndrome (CRS) (Fig. 2c). The cerebral symptoms were not relieved by a bolus infusion of mannitol. Lumbar puncture on day 5 showed an over 400-mmH2O cerebrospinal pressure. The cerebrospinal fluid (CSF) contained 20 WBCs/μL and 4.0 g/μL protein (Fig. 2d). CD3+ T cells were predominant in CSF, with few CD19+ B cells (Fig. 2e) which excluded CNSL. qPCR analysis for CAR construct showed 3,032,265 copies/μg DNA in CSF and 988,747 copies/μg DNA in blood. Cytokine levels in CSF were extremely higher than those in the serum, with IFN-γ at 2977 versus 152 pg/ml and IL-6 at 8475 versus 46 pg/ml (Fig. 2c). Methyprednisone was administrated at 3 mg/kg/day since day 5, and the symptoms relieved gradually. By day 9, all cerebral symptoms and signs disappeared, and serum IFN-γ and IL-6 levels decreased to normal ranges. Then, methyprednisone was de-escalated and tapered on day 14. The patient achieved complete remission (CR) with minimal residual disease (MRD) negative 10 days after CART19 infusion.
In the current study, the patient had experienced recurrent CNSL before CART19 infusion. Since CNS tissues lack CD19 expression , the CNS complications might less probably result from direct interactions between CD19+ cells and CART19. The predominance of CART19 in CSF and the huge discrepancies in inflammatory cytokine distributions strongly indicated the development of a cerebral CRS, presumably featured as CSF cytokines largely in situ produced by BBB-penetrating CAR T cells. Although no evidence of WBCs in CSF was observed before CART19 infusion, potent minimal residual CD19+ leukemia cells less than detection limits might enter CNS  and dramatically trigger activation of CAR T cells. Notably, this patient complicated severe cerebral CRS rather than systemic compromisations, suggesting that systemic and cerebral CRS were independent processes.
We have to acknowledge the comprehensive roles of CRS in CAR T cell therapy. Theoretically, cerebral CRS might facilitate elimination of CNSL and also cause severe CNS complications. Therefore, the timing and strategy to terminate CRS should be deliberately evaluated in clinical practice. Previous reports showed neurologic toxicities were not prevented by cytokine blockade via tocilizumab [6–8] due to its incapability of crossing the BBB. Our experience of using glucocorticoid as a salvage option could be instructive for a quick control of the severe CRS.
In all, we reported for the first time the development and control of cerebral CRS triggered by BBB-penetrating CAR T cells. This study provided insights into the etiology, diagnosis, and treatment of CRS after CAR T cell therapy.
ALL, acute lymphocytic leukemia; BBB, blood-brain barrier; BM, bone marrow; CAR T cell, chimeric antigen receptor-modified T cell; CART19, CD19-directed CAR T cell; CNSL, central nervous system leukemia; CR, complete remission; CRS, cytokine release syndrome; CSF, cerebrospinal fluid; MRD, minimal residual disease; PB, peripheral blood; WBCs, white blood cells
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This work was supported by the grants from the 973 Program (2015CB964900); Zhejiang Provincial Natural Science Foundation of China (LY14H080002, LY12H08002); the National Natural Science Foundation of China (81470341); Zhejiang Medical Technology and Education (2014KYA064, 2014KYA066); Key Project of Science and Technology Department of Zhejiang Province (2015C03G2010091); the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (CQ2014); and Beijing Municipal Administration of Hospitals’ Youth Programme (QML20150106, QML20150506). All funding sponsors had no influence in the design of the study and collection, analysis, and interpretation of the data and in writing the manuscript.
Availability of data and materials
The datasets supporting the conclusions of this article are included within the article and additional files.
HH, LX, and YH designed the study. HH, LX, YH, and QC performed the research and wrote the manuscript. JS, ZW, JY, CP, YL, JS, BL, and AJ performed the research. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
The authors have obtained consent to publish from the participant to report individual patient data.
Ethics approval and consent to participate
The protocol of the clinical trial ChiCTR-OCC-15007008 was reviewed and approved by the Institutional Review Board of the First Affiliated Hospital, School of Medicine, Zhejiang University (A2015008). All the enrolled patients provided written informed consent.
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Hu, Y., Sun, J., Wu, Z. et al. Predominant cerebral cytokine release syndrome in CD19-directed chimeric antigen receptor-modified T cell therapy. J Hematol Oncol 9, 70 (2016). https://doi.org/10.1186/s13045-016-0299-5