Fig. 2
Immunophenotype and transplantability of AML in FCN mice. (a–c) Immunophenotype of bone marrow. Relative frequency of LSK (a), LK (b), and myeloid populations (c) in total bone marrow (mean ± SD. n = 8 to 20). (d–e) CFU number from total bone marrow (d) or sorted LSK and LK (e) (mean ± SD. n = 3 to 9). f–k Analysis of mice transplanted with total bone marrow cells at different cell concentrations. Genotyping-PCR to assess leukemic engraftment depending on the total number of transplanted cells (f). Spleen weight of recipient animals as a function of the number of transplanted primary leukemia cells (g). Representative comparison of the frequencies of stem cells (LSK) and progenitor cells (LK) in leukemic donor and recipient mice, which had received 107 total donor cells (h). Proportions of LSK (i), LK (j), and CD11b+ (k) in the bone marrow of recipient mice, which have received 107 total donor cells (mean ± SD. n = 3 to 4). A one-way ANOVA or 2-way ANOVA with Bonferroni multiple comparison (a–e) or Dunnett’s post-test (g, i–k) was used to assess the significance of differences (p values: *p < 0.05, **p < 0.01, ***p < 0.001.)