Selective inhibitors of nuclear export (SINE)– a novel class of anti-cancer agents
© Parikh et al.; licensee BioMed Central Ltd. 2014
Received: 25 August 2014
Accepted: 9 October 2014
Published: 15 October 2014
Dysregulation of the nucleo-cytoplasmic transport of proteins plays an important role in carcinogenesis. The nuclear export of proteins depends on the activity of transport proteins, exportins. Exportins belong to the karyopherin β superfamily. Exportin-1 (XPO1), also known as chromosomal region maintenance 1 (CRM1), mediates transport of around 220 proteins. In this review, we summarized the development of a new class of antitumor drugs, collectively known as selective inhibitors of nuclear export (SINE). KPT-330 (selinexor) as an oral agent is showing activities in early clinical trials in both solid tumors and hematological malignancies.
The nucleo-cytoplasmic transport of proteins plays an important role in maintaining normal cellular functions. The nuclear export of proteins depends on the activity of transport proteins, exportins. Exportin-1 (XPO1), also known as chromosomal region maintenance 1 (CRM1), mediates transport of around 220 proteins -. XPO1 is the sole nuclear exporter of several tumor suppressor (TSP), growth regulatory (GRP) proteins. These include p53, p21, p73, Rb1, apc, bcr-abl, FOXO and STAT3. Under physiological conditions, the export of these proteins prevents them from overacting in the absence of DNA injury or other oncogenic activities ,. In cancerous cells, however, this export of proteins inhibits their tumor suppressor activity and promotes tumorigenesis ,. Many hematologic and solid tumor malignancies have elevated XPO1 levels -. Therefore, inhibiting XPO1 can be a potential treatment option. In this review, we will discuss a new class of potential antitumor drugs, collectively known as selective inhibitors of nuclear export (SINE). These agents can block the export of TSPs and GRPs, thus maintaining their intranuclear concentration and exert anti-cancer activity.
Nucleo-cytoplasmic transport and its inhibition by SINE compounds
A phase 1 study of an XPO1 inhibitor showed only modest efficacy and severe dose limiting toxicity (e.g. malaise, anorexia, vomiting and nausea) . The clinical trial was therefore discontinued. KOS-2462, a semisynthetic LMB derivative showed activity in mouse xenograft models without inducing significant toxicity . CBS9106 is another small molecule oral reversible inhibitor of XPO1. It induced growth inhibition in several cancer cell lines . Neither KOS-2462, nor CBS9106 have entered clinical trials.
Subsequently, several novel inhibitors of XPO1, collectively known as SINE compounds, have been developed. These compounds include KPT-330 (selinexor), KPT-335 (verdinexor), KPT-185, KPT-276, and KPT-251. Of these, KPT-185 is the most studied compound in vitro with most potency. However, its use in vivo is limited by poor pharmacokinetics -. KPT-330 is nearly as potent as KPT-185 and has acceptable oral bioavailability. KPT-251 and KPT-176 are not as potent as KPT-185 but are bioavailable orally -. These agents are currently undergoing clinical trials for several solid and hematologic malignancies including breast, colon, pancreas, renal, multiple myeloma (MM), mantle cell leukemia (MCL), chronic lymphocytic leukemia (CLL), and acute myeloid leukemia (AML).
SINE in pancreatic cancer
KPT-127, KPT-185, KPT- 205, and KPT-227 were studied in pancreatic cancer cell lines . Prostate apoptosis response-4 (PAR-4) is a proapoptotic protein in the nuclear and cytoplasmic compartments. PAR-4 translocates to the nucleus via XPO1 in external stress conditions to cause apoptosis . PAR-4 is downregulated in pancreatic cancers. Downregulation of PAR-4 directly correlates to worsening outcomes in pancreatic cancer . KPT-185 was shown to increase intranuclear PAR-4 without interfering with its import from the cytoplasm. It also induced PAR-4 phosphorylation, thus activating it and leading to apoptosis. Active SINEs had a median inhibitory concentration (IC50) of 150 nmol/L and inhibited pancreatic cancer cell lines while sparing normal human pancreatic ductal epithelial cells. The in vivo effects were noted using KPT-330 (selinexor) in subcutaneous and orthoptic pancreatic cancer models in mice. Oral administration of KPT-330 led to significant tumor growth inhibition when compared with control or gemcitabine treatment . KPT-330 treated mice had drastic reductions in tumor size as compared with controls. Thus, pre-clinical studies of CRM1 inhibition using SINE compounds revealed an attractive novel treatment of pancreatic cancer.
SINE in triple-negative breast cancer (TNBC) (ER−, PR−, Her2−)
Overexpression of survivin is associated with poor prognosis in breast cancer . Survivin inhibits apoptosis by stabilizing X linked inhibitor of apoptosis (XIAP) in the cytoplasm . Survivin expression is also directly affected by STAT3, a member of Janus-activated kinase (JAK)/STAT , which is increased in several malignancies including TNBC . Cytoplasmic localization is required for survivin to inhibit apoptosis . XPO1 mediates transport of survivin and STAT3 to the cytoplasm, and inhibits apoptosis ,. Inhibition of XPO1 blocked STAT3 binding to survivin promoter and decreased survivin expression. In the meanwhile, it was shown that survivin was cleaved by caspase-3, therefore leading to overall decrease of survivin level . In the study, it was shown that KPT-185, KPT-251 and KPT-276 inhibited tumor cell growth and enhanced apoptosis in vitro in 3 different cell lines. KPT-185cis had the lowest IC50. KPT-330 had profound effects on tumor cell growth inhibition and apoptosis with an IC50 ranging from 5 to 21 nmol/L. The data suggested that twice weekly dosing of KPT-330 at 25 mg/kg for 42 days significantly reduced tumor growth when compared to control or standard treatment with 5-fluorouracil (P = 0.011). It was determined that XPO1 inhibition caused nuclear retention of survivin which was then degraded by caspase-3 . Survivin transcription was also shown to be repressed by inhibition of CREB binding protein (CBP) mediated STAT3 transactivation.
This study expands the role of SINEs in treatment of breast cancer and other solid tumors. KPT-330 is currently undergoing phase I clinical trial in advanced solid tumors . In this early trial, KPT-330 was administered orally for 8–10 doses in a 28-day cycles to 103 patients (59/44 M/F; median age 61 years) across 12 dose levels. Dose limiting toxicites (DLT) (fatigue, dehydration, nausea) were noted. Dosing at 65 mg/m2 BIW is ongoing since maximal tolerated dosage (MTD) was not reached yet at the time of the report. There were 87 evaluable patients (pts) for response. Among them, there were 3 PR in colorectal cancer (KRAS mutant), melanoma (BRAFwt) and ovarian adenocarcinoma pts. Stable disease (SD) was seen in 39 pts, with 12 pts lasting over 6 months. All 5 evaluable pts with hormone and chemotherapy refractory prostate cancer (HRPC) achieved SD; Nine of 13 evaluable pts with squamous head and neck cancer had SD diseases. Further evaluations are ongoing.
SINE in non-small cell lung cancer (NSCLC)
Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKI) are main treatment for patients with advanced NSCLC with EGFR exon 19 deletion or exon 21 substitution ,. EGFR overexpression and p53 mutations are associated with poor outcomes in NSCLC ,. As mentioned earlier, nuclear export of p53 is mediated by XPO1 . Sun et al. studied the antitumor activity of KPT-330 against NSCLC in vitro and in vivo, and concluded that the antitumor activity of KPT-330 against NSCLC was independent of p53 mutational status . The antitumor activity of KPT-330 against NSCLC was likely related to p73. p73 shares structural and functional similarities with p53 and cooperates with p53 to induce apoptosis . In cells with mutant p53, p73 is shown to cause apoptosis via activation of p53-inducible genes . KPT-330 caused dose-dependent growth inhibition of NSCLC with correlating decrease in XPO1 levels . Moreover, KPT-330 can inhibit NSCLC cell growth even in EGFR-TKI resistant cancer cells. Combination of KPT-330 and cisplatin displayed synergistic in vitro antiproliferative activity. In vivo treatment of mice with a dose of 10 mg/kg, thrice weekly for 4 weeks showed significant tumor growth inhibition with minimal toxicities. Another independent study confirmed the above findings using KPT-185 in vitro and its oral clinical equivalent KPT-276 on NSCLC cells in vivo using mouse xenografts .
SINE in renal cell carcinoma (RCC)
Despite several approved drugs for metastatic RCC, the progression free survival remains only 1 to 2 years . KPT-185 was studied in vitro in RCC cell lines . XPO1 is overexpressed in high grade RCC. KPT-185 and its oral equivalent KPT-251 decreased XPO1 levels. They also compared SINE with sorafinib and found greater inhibition of tumor growth with KPT-251 at a higher dose of 75 mg/kg in mouse xenografts (p = 0.07) without any adverse effects. KPT-185 increased nuclear localization of p53 and its downstream protein p21 to cause cell cycle arrest. Also, cytosol p21 reduction leads to apoptosis. KPT-251 showed increased p53 and p21 nuclear levels in vivo. Sorafinib, on the other hand, decreased nuclear and cytoplasmic p21, thereby causing apoptosis . These findings warrant further clinical studies using SINE as a treatment choice in RCC.
SINE in melanoma
BRAF kinase activation is present in about 50% of melanomas -. Treatment with BRAF and MEK inhibitors has been very successful, but the eventual development of resistance to these kinase inhibitors calls for more agents ,-. XPO1 expression is found to be increased in metastatic melanoma more than primary melanoma or nevi . Hence, SINE can be a potential treatment for metastatic melanoma. Salas Fragomeni et al. conducted in vitro and in vivo studies in metastatic melanoma using SINE and BRAF inhibitors . They concluded that BRAF inhibition by PLX-432 led to inhibition of cell proliferation in BRAF-mutant cell lines, but BRAF wild-type (WT) melanoma cell lines were relatively resistant ,. However, SINEs inhibited cell proliferation and caused cytotoxicity across all cell lines, regardless of BRAF status. They also showed synergistic activity between SINE and PLX-432 in BRAF mutant melanoma. Furthermore, SINE and MEK inhibitors were also able to synergize with the three-way treatment (SINE/MEK/BRAF), achieving an even lower IC50. SINE also caused G1/S phase cell cycle arrest, another effect synergized with concomitant BRAF inhibition in BRAF mutant cell lines. SINE also increased nuclear p53, retinoblastoma (Rb). An increase in ERK phosphorylation in the nucleus was also noticed. ERK phosphorylation has been linked to increased cellular proliferation and development of chemoresistance ,,. However, this SINE- induced ERK phosphorylation was prevented in the presence of BRAF inhibition, possibly explaining the synergy between these two compounds. In vivo mouse model showed complete tumor regression using the combination regimen .
SINE in acute leukemia
SINE compounds have been studied as a novel anticancer strategy in multiple preclinical trials of hematologic malignancies. Higher levels of XPO1 are associated with poor prognosis in acute leukemia . Earlier preclinical work demonstrated that KPT-185 inhibited proliferation and induced G1 phase cell cycle arrest in AML cell lines and primary AML blasts in vitro. Nucleophosmin 1 (NPM1) is a nucleolar TSP that shuttles between the nucleolus and cytoplasm via the XPO1-RanGTP pathway and regulates p53 dependent cell death . NPM1 mutations in AML cells were seen in 25% to 35% cases . These mutations cause increased XPO1 binding and localization of NPM1 in the cytoplasm . SINEs block this export of mutant NPM1 and induce antileukemic effects in AML cell lines and primary AML blasts. AML blasts with NPM1 mutations were very responsive to KPT-185 and had an IC50 of 100 nmol/L. However, wild type (WT) NPM1 in AML cells were also sensitive to SINEs, indicating that other TSPs like p53 also have a role in the antileukemic effects of SINE. Kojima et al. further established that p53 is a major determinant in SINE induced cytotoxicity in AML, independent of NPM1. Mutant p53 samples were less sensitive to KPT-185 . XPO1 inhibition by SINE also resulted in blast differentiation, likely due to upregulation by p53 and CEBPA , a protein essential for myeloid granulocytic differentiation via activation of several necessary genes ,. SINEs were also shown to downregulate FLT3 and cKIT tyrosine kinase proteins . FLT3 gene mutation may coexist with NPM1 mutations . SINE downregulated FLT3 and NPM1. Thus, SINEs can potentially target 2 critical pathways. cKIT mutations or overexpression also confer a worse prognosis in AML ,. Kojima et al. demonstrated synergistic activity using the combination of SINE with MDM2 inhibitor Nutlin-3a. MDM2, frequently overexpressed in AML, is a p53-specific ligase, promoting p53 degradation . Nutlin-3a is a selective MDM2 inhibitor, shown to increase nuclear and cytoplasmic p53 and induces p53 mediated apoptosis . The addition of SINE to Nutlin-3a led to higher p53 nuclear level than by using either agent alone in vitro. This combination strategy can be potentially effective not only in AML but in several other malignancies. Furthermore, SINEs are not shown to induce apoptosis in normal hematopoietic cells ,,.
T cell-acute lymphoblastic leukemia (T-ALL) is fatal in about 50-70% of adult patients -. SINE has shown striking activity in preclinical study for treatment of T-ALL. KPT-185 and KPT-330 showed rapid apoptosis induction in T-ALL cell lines in vitro with acceptable IC50. In vivo treatment showed minimal gastrointestinal adverse effects.
Philadelphia chromosome positive ALL remains a challenge even with availability of multiple tyrosine kinase inhibitors ,. Walker et al. demonstrated successful use of KPT-185 in vitro and KPT-330 in vivo in Philadelphia chromosome positive ALL (Ph + ALL) and chronic myeloid leukemia blast crisis (CML-BC). Combination with imatinib led to synergistic effects . SINE treatment was associated with significant reduction in BCR-ABL + cells in mice, likely by reactivation of the tumor suppressor proteins PP2A, p53, p21 and FOXO3a .
The preliminary results of an ongoing phase I trial using KPT-330 in relapsed/refractory AML were presented Yee et al. recently. They showed that KPT-330 treatment given to heavily pretreated, refractory/relapsed AML patients had no DLT. Out of the 32 evaluable patients, 4 (12%) showed complete response (CR) with hematological recovery, 1 (3%) showed marrow CR (mCR), mCR without hematological recovery was seen in 1 (3%) patient. Partial response (PR) was seen in 2 patients (6%). Eleven patients (34%) showed progression while 12 (37%) experienced stable disease after 30 days .
SINE in chronic leukemia
Even though more options are available now for chronic lymphocytic leukemia (CLL) therapy, p53 positive CLL still has poor prognosis -. Preclinical study by Lapalombella et al. using KPT-185 in vitro and KPT-251 in vivo showed promising results in chronic lymphocytic leukemia (CLL) cells . KPT-185 induced nuclear retention of IκB. IκB is an endogenous inhibitor of the inflammatory antiapoptotic transcription factor NFκB, which is involved in the upregulation of MCL1, the most significant antiapoptotic protein associated with CLL ,. KPT-185 induced MCL1 depletion, likely due to inactivation of NFκB by nuclear retention of IκB. Murine xenografts treated with KPT-251 showed significant improvement in survival when compared with fludarabine . In a 37 year old patient with CML-AP resistant to multiple treatment options (TKIs, interferon, omacetaxine and azacitidine), a trial of selinexor (KPT-330) on a compassionate use protocol showed significant reduction in bone pain, spleen size, white blood cell count, and lactate dehydrogenase (LDH) level. Peripheral blood smears showed a dramatic reduction in immature myeloid blasts .
SINE in multiple myeloma (MM)
SINE was shown to induce cytotoxicity and inhibits osteoclastogenesis in multiple myeloma in vitro and in vivo. High CRM1 expression was found to be associated with lytic bone disease (P = 0.008) and shorter survival (P = 0.024). CRM1 levels are higher in bortezomib resistant MM cells. SINEs caused nuclear accumulation of multiple TSPs including p53, FOXO3a, IκB, p21 and PP2A. SINE also caused anti-MM effects in the bone marrow microenvironment by activating caspase cascade and causing PARP cleavage, and showed a synergistic effect when combined with bortezomib, without affecting bone marrow stromal cells. Another study, however, observed no synergy with KPT-276 in combination with dexamethasone, bortezomib, or melphalan . Osteoclastogenesis is controlled by NFκB activation through cytokine RANKL and NFAT1c. Both KTP-185 and KPT-330 blocked RANKL mediated activation of NFκB in osteoclast (OC) precursor cells, and also blocked NKFAT1c, which is also essential for osteoclast function ,. Surprisingly, SINE reduced the expression of oncogene c-myc, despite the fact that CRM1 does not mediate c-myc export . C-myc activation is associated with poor prognosis and shorter survival in monoclonal gammopathy of undetermined significance (MGUS) and MM . A study done in 2012 indicated that p53 activation is responsible for inhibiting CRM1 and c-myc genes . BRD4, another gene downregulated by SINE, regulates DNA replication, promotes c-myc transcription and is associated with MM disease progression ,. BRD4 knockdown causes cell cycle arrest and subsequent apoptosis . JQ1, a small molecule inhibitor of BRD4 gene causes decreased transcription of c-myc, an effect that was synergistic with KPT-276 . This result can be utilized for further cytotoxic treatment of tumor cells in MM. Lastly, 2 week in vivo treatment with KPT-276 had a comparable effect on M-spike reduction with melphalan and bortezomib, the two potent anti-MM drugs .
SINE in lymphoma
Novel agents and regimens for lymphoma are moving rapidly from bench to bedside ,-. KPT drugs have been studied preclinically for the treatment of resistant mantle cell lymphoma (MCL). Yoshimura et al. conducted an in vitro study of KPT-185 in MCL and showed that SINE increases MCL cell apoptosis primarily by increasing nuclear p53 levels . They verified that KPT-185 downregulated c-myc and NFκB, thus targeting multiple pathways of apoptosis. Zhang et al. studied the in vivo effects of KPT-276 in mice and showed marked activity with minimal weight loss, gastrointestinal side effects, or myelosuppression . London et al. studied SINEs in vivo in a phase I clinical trial in spontaneous canine NHL, osterosarcoma or mast cell tumor. They used KPT-335 at a dose of 1 mg/kg to 1.75 mg/kg two times a week and showed significant response without development of serious side effects . Gutierrez et al. presented the findings of their phase I study with KPT-330 in 32 pretreated refractory lymphoma patients. The optimal dosing of KPT-330 is at least 45 mg/m2 and durable activity of KPT-330 was observed in those NHL patients . These data further validated the activity of SINEs in human malignancies.
Selinexor (KPT-330) in clinical trials
Relapsed ALL and AML
Not yet Recruiting
Advanced/metastatic solid tumors
Soft-tissue or bone sarcoma
Advanced/metastatic solid tumors
Metastatic resistant prostate cancer
Advanced hematological malignancies
Acute myeloid leukemia
Locally advanced rectal cancer
Not yet Recruiting
DL and KP designed the study. All authors have contributed to data preparation, drafting and revising the manuscripts. All authors have read and approved the final manuscript.
Dr. Shundong Cang is a recipient of CAHON Scholar award (http://www.CAHON.org) and was partly funded by International Scholar Exchange Foundation.
- Fukuda M, Asano S, Nakamura T, Adachi M, Yoshida M, Yanagida M, Nishida E: CRM1 is responsible for intracellular transport mediated by the nuclear export signal. Nature. 1997, 390: 308-311.PubMedGoogle Scholar
- Xu D, Grishin NV, Chook YM: NESdb: a database of NES-containing CRM1 cargoes. Mol Biol Cell. 2012, 23: 3673-3676.PubMed CentralPubMedGoogle Scholar
- Nguyen KT, Holloway MP, Altura RA: The CRM1 nuclear export protein in normal development and disease. Int J Biochem Mol Biol. 2012, 3: 137-151.PubMed CentralPubMedGoogle Scholar
- Cheng Y, Holloway MP, Nguyen K, McCauley D, Landesman Y, Kauffman MG, Shacham S, Altura RA: XPO1 (CRM1) inhibition represses STAT3 activation to drive a survivin-dependent oncogenic switch in triple-negative breast cancer. Mol Cancer Ther. 2014, 13: 675-686.PubMed CentralPubMedGoogle Scholar
- Kutay U, Hetzer MW: Reorganization of the nuclear envelope during open mitosis. Curr Opin Cell Biol. 2008, 20: 669-677.PubMed CentralPubMedGoogle Scholar
- Turner JG, Sullivan DM: CRM1-mediated nuclear export of proteins and drug resistance in cancer. Curr Med Chem. 2008, 15: 2648-2655.PubMedGoogle Scholar
- Turner JG, Dawson J, Sullivan DM: Nuclear export of proteins and drug resistance in cancer. Biochem Pharmacol. 2012, 83: 1021-1032.PubMed CentralPubMedGoogle Scholar
- Noske A, Weichert W, Niesporek S, Röske A, Buckendahl AC, Koch I, Sehouli J, Dietel M, Denkert C: Expression of the nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 is a prognostic factor in human ovarian cancer. Cancer. 2008, 112: 1733-1743.PubMedGoogle Scholar
- Shen A, Wang Y, Zhao Y, Zou L, Sun L, Cheng C: Expression of CRM1 in human gliomas and its significance in p27 expression and clinical prognosis. Neurosurgery. 2009, 65: 153-159. discussion 159–160PubMedGoogle Scholar
- Yao Y, Dong Y, Lin F, Zhao H, Shen Z, Chen P, Sun YJ, Tang LN, Zheng SE: The expression of CRM1 is associated with prognosis in human osteosarcoma. Oncol Rep. 2009, 21: 229-235.PubMedGoogle Scholar
- Huang WY, Yue L, Qiu WS, Wang LW, Zhou XH, Sun YJ: Prognostic value of CRM1 in pancreas cancer. Clin Invest Med. 2009, 32: E315-PubMedGoogle Scholar
- Tai YT, Landesman Y, Acharya C, Calle Y, Zhong MY, Cea M, Tannenbaum D, Cagnetta A, Reagan M, Munshi AA, Senapedis W, Saint-Martin JR, Kashyap T, Shacham S, Kauffman M, Gu Y, Wu L, Ghobrial I, Zhan F, Kung AL, Schey SA, Richardson P, Munshi NC, Anderson KC: CRM1 inhibition induces tumor cell cytotoxicity and impairs osteoclastogenesis in multiple myeloma: molecular mechanisms and therapeutic implications. Leukemia. 2014, 28: 155-165.PubMed CentralPubMedGoogle Scholar
- Güttler T, Görlich D: Ran-dependent nuclear export mediators: a structural perspective. EMBO J. 2011, 30: 3457-3474.PubMed CentralPubMedGoogle Scholar
- Monecke T, Güttler T, Neumann P, Dickmanns A, Gürlich D, Ficner R: Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP. Science. 2009, 324: 1087-1091.PubMedGoogle Scholar
- Fung HY, Chook YM: Atomic basis of CRM1-cargo recognition, release and inhibition.Semin Cancer Biol 2014, ., [http://dx.doi.org/10.1016/j.semcancer.2014.03.002]
- Mutka SC, Yang WQ, Dong SD, Ward SL, Craig DA, Timmermans PB, Murli S: Identification of nuclear export inhibitors with potent anticancer activity in vivo. Cancer Res. 2009, 69: 510-517.PubMed CentralPubMedGoogle Scholar
- Sakakibara K, Saito N, Sato T, Suzuki A, Hasegawa Y, Friedman JM, Kufe DW, Vonhoff DD, Iwami T, Kawabe T: CBS9106 is a novel reversible oral CRM1 inhibitor with CRM1 degrading activity. Blood. 2011, 118: 3922-3931.PubMedGoogle Scholar
- Kudo N, Matsumori N, Taoka H, Fujiwara D, Schreiner EP, Wolff B, Yoshida M, Horinouchi S: Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. Proc Natl Acad Sci U S A. 1999, 96: 9112-9117.PubMed CentralPubMedGoogle Scholar
- Bonazzi S, Eidam O, Güttinger S, Wach JY, Zemp I, Kutay U, Gademann K: Anguinomycins and derivatives: total syntheses, modeling, and biological evaluation of the inhibition of nucleocytoplasmic transport. J Am Chem Soc. 2010, 132: 1432-1442.PubMedGoogle Scholar
- Daelemans D, Afonina E, Nilsson J, Werner G, Kjems J, De Clercq E, Pavlakis GN, Vandamme AM: A synthetic HIV-1 Rev inhibitor interfering with the CRM1-mediated nuclear export. Proc Natl Acad Sci U S A. 2002, 99: 14440-14445.PubMed CentralPubMedGoogle Scholar
- Meissner T, Krause E, Vinkemeier U: Ratjadone and leptomycin B block CRM1-dependent nuclear export by identical mechanisms. FEBS Lett. 2004, 576: 27-30.PubMedGoogle Scholar
- Newlands ES, Rustin GJ, Brampton MH: Phase I trial of elactocin. Br J Cancer. 1996, 74: 648-649.PubMed CentralPubMedGoogle Scholar
- Etchin J, Sun Q, Kentsis A, Farmer A, Zhang ZC, Sanda T, Mansour MR, Barcelo C, McCauley D, Kauffman M, Shacham S, Christie AL, Kung AL, Rodig SJ, Chook YM, Look AT: Antileukemic activity of nuclear export inhibitors that spare normal hematopoietic cells. Leukemia. 2013, 27: 66-74.PubMed CentralPubMedGoogle Scholar
- Lapalombella R, Sun Q, Williams K, Tangeman L, Jha S, Zhong Y, Goettl V, Mahoney E, Berglund C, Gupta S, Farmer A, Mani R, Johnson AJ, Lucas D, Mo X, Daelemans D, Sandanayaka V, Shechter S, McCauley D, Shacham S, Kauffman M, Chook YM, Byrd JC: Selective inhibitors of nuclear export show that CRM1/XPO1 is a target in chronic lymphocytic leukemia. Blood. 2012, 120: 4621-4634.PubMed CentralPubMedGoogle Scholar
- Ranganathan P, Yu X, Na C, Santhanam R, Shacham S, Kauffman M, Walker A, Klisovic R, Blum W, Caligiuri M, Croce CM, Marcucci G, Garzon R: Preclinical activity of a novel CRM1 inhibitor in acute myeloid leukemia. Blood. 2012, 120: 1765-1773.PubMed CentralPubMedGoogle Scholar
- Azmi AS, Aboukameel A, Bao B, Sarkar FH, Philip PA, Kauffman M, Shacham S, Mohammad RM: Selective inhibitors of nuclear export block pancreatic cancer cell proliferation and reduce tumor growth in mice. Gastroenterology. 2013, 144: 447-456.PubMed CentralPubMedGoogle Scholar
- Sells SF, Han SS, Muthukkumar S, Maddiwar N, Johnstone R, Boghaert E, Gillis D, Liu G, Nair P, Monnig S, Collini P, Mattson MP, Sukhatme VP, Zimmer SG, Wood DP, McRoberts JW, Shi Y, Rangnekar VM: Expression and function of the leucine zipper protein Par-4 in apoptosis. Mol Cell Biol. 1997, 17: 3823-3832.PubMed CentralPubMedGoogle Scholar
- Ahmed MM, Sheldon D, Fruitwala MA, Venkatasubbarao K, Lee EY, Gupta S, Wood C, Mohiuddin M, Strodel WE: Downregulation of PAR-4, a pro-apoptotic gene, in pancreatic tumors harboring K-ras mutation. Int J Cancer. 2008, 122: 63-70.PubMedGoogle Scholar
- Tanaka K, Iwamoto S, Gon G, Nohara T, Iwamoto M, Tanigawa N: Expression of survivin and its relationship to loss of apoptosis in breast carcinomas. Clin Cancer Res. 2000, 6: 127-134.PubMedGoogle Scholar
- Dohi T, Okada K, Xia F, Wilford CE, Samuel T, Welsh K, Marusawa H, Zou H, Armstrong R, Matsuzawa S, Zou H, Armstrong R, Matsuzawa S, Salvesen GS, Reed JC, Altieri DC: An IAP-IAP complex inhibits apoptosis. J Biol Chem. 2004, 279: 34087-34090.PubMedGoogle Scholar
- Furqan M, Mukhi N, Lee B, Liu D: Dysregulation of JAK-STAT pathway in hematological malignancies and JAK inhibitors for clinical application. Biomarker Res. 2013, 1: 5-Google Scholar
- Bromberg J, Darnell JE: The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000, 19: 2468-2473.PubMedGoogle Scholar
- Rodríguez JA, Span SW, Ferreira CG, Kruyt FA, Giaccone G: CRM1-mediated nuclear export determines the cytoplasmic localization of the antiapoptotic protein Survivin. Exp Cell Res. 2002, 275: 44-53.PubMedGoogle Scholar
- Stauber RH, Rabenhorst U, Rekik A, Engels K, Bier C, Knauer SK: Nucleocytoplasmic shuttling and the biological activity of mouse survivin are regulated by an active nuclear export signal. Traffic. 2006, 7: 1461-1472.PubMedGoogle Scholar
- Mau-Soerensen M, Razak ARA, Shields AF, Gabrail NY, Gerecitano JF, Shacham S, Lassen UN, Rashal T, Cooksey J, Landesman Y, Pond G, Oza AM, Kauffman M, Siu LL, Bedard PL, Mahaseth H, Mirza MR, Mahipal A: A first-in-class, first-in-human phase I trial of KPT-330 (selinexor), a selective inhibitor of nuclear export (SINE) in patients (pts) with advanced solid tumors. ASCO Meeting Abstracts. 2014, 32: 2537-Google Scholar
- Xu C, Zhou Q, Wu Y-l: Can EGFR-TKIs be used in first line treatment for advanced non-small cell lung cancer based on selection according to clinical factors? – A literature-based meta-analysis. J Hematol Oncol. 2012, 5: 62-PubMed CentralPubMedGoogle Scholar
- Niu F-Y, Wu Y-L: Novel agents and strategies for overcoming EGFR TKIs resistance. Exp Hematol Oncol. 2014, 3: 2-PubMed CentralPubMedGoogle Scholar
- Sharma SV, Bell DW, Settleman J, Haber DA: Epidermal growth factor receptor mutations in lung cancer. Nat Rev Cancer. 2007, 7: 169-181.PubMedGoogle Scholar
- Stewart DJ: Tumor and host factors that may limit efficacy of chemotherapy in non-small cell and small cell lung cancer. Crit Rev Oncol Hematol. 2010, 75: 173-234.PubMed CentralPubMedGoogle Scholar
- Stommel JM, Marchenko ND, Jimenez GS, Moll UM, Hope TJ, Wahl GM: A leucine-rich nuclear export signal in the p53 tetramerization domain: regulation of subcellular localization and p53 activity by NES masking. EMBO J. 1999, 18: 1660-1672.PubMed CentralPubMedGoogle Scholar
- Sun H, Hattori N, Chien W, Sun Q, Sudo M, GL EL, Ding L, Lim SL, Shacham S, Kauffman M, Nakamaki T, Koeffler HP: KPT-330 has antitumour activity against non-small cell lung cancer. Br J Cancer. 2014, 111: 281-291.PubMed CentralPubMedGoogle Scholar
- Moll UM, Slade N: p63 and p73: roles in development and tumor formation. Mol Cancer Res. 2004, 2: 371-386.PubMedGoogle Scholar
- Wang S, Han X, Wang J, Yao J, Shi Y: Antitumor effects of a novel chromosome region maintenance 1 (CRM1) inhibitor on non-small cell lung cancer cells in vitro and in mouse tumor xenografts. PLoS One. 2014, 9: e89848-PubMed CentralPubMedGoogle Scholar
- Belldegrun AS, Klatte T, Shuch B, LaRochelle JC, Miller DC, Said JW, Riggs SB, Zomorodian N, Kabbinavar FF, Dekernion JB, Pantuck AJ: Cancer-specific survival outcomes among patients treated during the cytokine era of kidney cancer (1989–2005): a benchmark for emerging targeted cancer therapies. Cancer. 2008, 113: 2457-2463.PubMedGoogle Scholar
- Inoue H, Kauffman M, Shacham S, Landesman Y, Yang J, Evans CP, Weiss RH: CRM1 blockade by selective inhibitors of nuclear export attenuates kidney cancer growth. J Urol. 2013, 189: 2317-2326.PubMed CentralPubMedGoogle Scholar
- Inoue H, Hwang SH, Wecksler AT, Hammock BD, Weiss RH: Sorafenib attenuates p21 in kidney cancer cells and augments cell death in combination with DNA-damaging chemotherapy. Cancer Biol Ther. 2011, 12: 827-836.PubMed CentralPubMedGoogle Scholar
- Jerant AF, Johnson JT, Sheridan CD, Caffrey TJ: Early detection and treatment of skin cancer. Am Fam Physician. 2000, 62: 357-368. 375–356, 381–352PubMedGoogle Scholar
- Atefi M, von Euw E, Attar N, Ng C, Chu C, Guo D, Nazarian R, Chmielowski B, Glaspy JA, Comin-Anduix B, Mischel PS, Lo RS, Ribas A: Reversing melanoma cross-resistance to BRAF and MEK inhibitors by co-targeting the AKT/mTOR pathway. PLoS One. 2011, 6: e28973-PubMed CentralPubMedGoogle Scholar
- Baldus SE, Schaefer KL, Engers R, Hartleb D, Stoecklein NH, Gabbert HE: Prevalence and heterogeneity of KRAS, BRAF, and PIK3CA mutations in primary colorectal adenocarcinomas and their corresponding metastases. Clin Cancer Res. 2010, 16: 790-799.PubMedGoogle Scholar
- Dalle S, Poulalhon N, Thomas L: Vemurafenib in melanoma with BRAF V600E mutation. N Engl J Med. 2011, 365: 1448-1449. author reply 1450PubMedGoogle Scholar
- Davies H, Bignell GR, Cox C, Stephens P, Edkins S, Clegg S, Teague J, Woffendin H, Garnett MJ, Bottomley W, Davis N, Dicks E, Ewing R, Floyd Y, Gray K, Hall S, Hawes R, Hughes J, Kosmidou V, Menzies A, Mould C, Parker A, Stevens C, Watt S, Hooper S, Wilson R, Jayatilake H, Gusterson BA, Cooper C, Shipley J: Mutations of the BRAF gene in human cancer. Nature. 2002, 417: 949-954.PubMedGoogle Scholar
- Lee B, Mukhi N, Liu D: Current management and novel agents for malignant melanoma. J Hematol Oncol. 2012, 5: 3-PubMed CentralPubMedGoogle Scholar
- Akinleye A, Furqan M, Mukhi N, Ravella P, Liu D: MEK and the inhibitors: from bench to bedside. J Hematol Oncol. 2013, 6: 27-PubMed CentralPubMedGoogle Scholar
- Huang T, Karsy M, Zhuge J, Zhong M, Liu D: B-Raf and the inhibitors: from bench to bedside. J Hematol Oncol. 2013, 6: 30-PubMed CentralPubMedGoogle Scholar
- Ascierto PA, Schadendorf D, Berking C, Agarwala SS, van Herpen CM, Queirolo P, Blank CU, Hauschild A, Beck JT, St-Pierre A, Niazi F, Wandel S, Peters M, Zubel A, Dummer R: MEK162 for patients with advanced melanoma harbouring NRAS or Val600 BRAF mutations: a non-randomised, open-label phase 2 study. Lancet Oncol. 2013, 14: 249-256.PubMedGoogle Scholar
- Chapman PB, Hauschild A, Robert C, Haanen JB, Ascierto P, Larkin J, Dummer R, Garbe C, Testori A, Maio M, Hogg D, Lorigan P, Lebbe C, Jouary T, Schadendorf D, Ribas A, O’Day SJ, Sosman JA, Kirkwood JM, Eggermont AM, Dreno B, Nolop K, Li J, Nelson B, Hou J, Lee RJ, Flaherty KT, McArthur GA: Improved survival with vemurafenib in melanoma with BRAF V600E mutation. N Engl J Med. 2011, 364: 2507-2516.PubMed CentralPubMedGoogle Scholar
- Flaherty KT, Robert C, Hersey P, Nathan P, Garbe C, Milhem M, Demidov LV, Hassel JC, Rutkowski P, Mohr P, Dummer R, Trefzer U, Larkin JM, Utikal J, Dreno B, Nyakas M, Middleton MR, Becker JC, Casey M, Sherman LJ, Wu FS, Ouellet D, Martin AM, Patel K, Schadendorf D: Improved survival with MEK inhibition in BRAF-mutated melanoma. N Engl J Med. 2012, 367: 107-114.PubMedGoogle Scholar
- Ho AL, Grewal RK, Leboeuf R, Sherman EJ, Pfister DG, Deandreis D, Pentlow KS, Zanzonico PB, Haque S, Gavane S, Ghossein RA, Ricarte-Filho JC, Dominguez JM, Shen R, Tuttle RM, Larson SM, Fagin JA: Selumetinib-enhanced radioiodine uptake in advanced thyroid cancer. N Engl J Med. 2013, 368: 623-632.PubMed CentralPubMedGoogle Scholar
- Janne PA, Shaw AT, Pereira JR, Jeannin G, Vansteenkiste J, Barrios C, Franke FA, Grinsted L, Zazulina V, Smith P, Crino L: Selumetinib plus docetaxel for KRAS-mutant advanced non-small-cell lung cancer: a randomised, multicentre, placebo-controlled, phase 2 study. Lancet Oncol. 2013, 14: 38-47.PubMedGoogle Scholar
- Marks JL, Gong Y, Chitale D, Golas B, McLellan MD, Kasai Y, Ding L, Mardis ER, Wilson RK, Solit D, Levine R, Michel K, Thomas RK, Rusch VW, Ladanyi M, Pao W: Novel MEK1 mutation identified by mutational analysis of epidermal growth factor receptor signaling pathway genes in lung adenocarcinoma. Cancer Res. 2008, 68: 5524-5528.PubMed CentralPubMedGoogle Scholar
- Pathria G, Wagner C, Wagner SN: Inhibition of CRM1-mediated nucleocytoplasmic transport: triggering human melanoma cell apoptosis by perturbing multiple cellular pathways. J Invest Dermatol. 2012, 132: 2780-2790.PubMedGoogle Scholar
- Salas Fragomeni RA, Chung HW, Landesman Y, Senapedis W, Saint-Martin JR, Tsao H, Flaherty KT, Shacham S, Kauffman M, Cusack JC: CRM1 and BRAF inhibition synergize and induce tumor regression in BRAF-mutant melanoma. Mol Cancer Ther. 2013, 12: 1171-1179.PubMedGoogle Scholar
- Cagnol S, Chambard JC: ERK and cell death: mechanisms of ERK-induced cell death–apoptosis, autophagy and senescence. FEBS J. 2010, 277: 2-21.PubMedGoogle Scholar
- Mirmohammadsadegh A, Mota R, Gustrau A, Hassan M, Nambiar S, Marini A, Bojar H, Tannapfel A, Hengge UR: ERK1/2 is highly phosphorylated in melanoma metastases and protects melanoma cells from cisplatin-mediated apoptosis. J Invest Dermatol. 2007, 127: 2207-2215.PubMedGoogle Scholar
- Kojima K, Kornblau SM, Ruvolo V, Dilip A, Duvvuri S, Davis RE, Zhang M, Wang Z, Coombes KR, Zhang N, Qiu YH, Burks JK, Kantarjian H, Shacham S, Kauffman M, Andreeff M: Prognostic impact and targeting of CRM1 in acute myeloid leukemia. Blood. 2013, 121: 4166-4174.PubMed CentralPubMedGoogle Scholar
- Saha M, Qiu L, Chang H: Targeting p53 by small molecules in hematological malignancies. J Hematol Oncol. 2013, 6: 23-PubMed CentralPubMedGoogle Scholar
- Pawar R, Singh Bali OP, Malhotra BK, Lamba G: Recent advances and novel agents for FLT3 mutated AML. Stem Cell Invest. 2014, 1: 7-Google Scholar
- Cheng K, Sportoletti P, Ito K, Clohessy JG, Teruya-Feldstein J, Kutok JL, Pandolfi PP: The cytoplasmic NPM mutant induces myeloproliferation in a transgenic mouse model. Blood. 2010, 115: 3341-3345.PubMed CentralPubMedGoogle Scholar
- Yoon K, Smart RC: C/EBPalpha is a DNA damage-inducible p53-regulated mediator of the G1 checkpoint in keratinocytes. Mol Cell Biol. 2004, 24: 10650-10660.PubMed CentralPubMedGoogle Scholar
- Keeshan K, Santilli G, Corradini F, Perrotti D, Calabretta B: Transcription activation function of C/EBPalpha is required for induction of granulocytic differentiation. Blood. 2003, 102: 1267-1275.PubMedGoogle Scholar
- Tenen DG, Hromas R, Licht JD, Zhang DE: Transcription factors, normal myeloid development, and leukemia. Blood. 1997, 90: 489-519.PubMedGoogle Scholar
- Mrózek K, Marcucci G, Paschka P, Whitman SP, Bloomfield CD: Clinical relevance of mutations and gene-expression changes in adult acute myeloid leukemia with normal cytogenetics: are we ready for a prognostically prioritized molecular classification?. Blood. 2007, 109: 431-448.PubMed CentralPubMedGoogle Scholar
- Ikeda H, Kanakura Y, Tamaki T, Kuriu A, Kitayama H, Ishikawa J, Kanayama Y, Yonezawa T, Tarui S, Griffin JD: Expression and functional role of the proto-oncogene c-kit in acute myeloblastic leukemia cells. Blood. 1991, 78: 2962-2968.PubMedGoogle Scholar
- Paschka P, Marcucci G, Ruppert AS, Mrózek K, Chen H, Kittles RA, Vukosavljevic T, Perrotti D, Vardiman JW, Carroll AJ, Kolitz JE, Larson RA, Bloomfield CD, CaLG B: Adverse prognostic significance of KIT mutations in adult acute myeloid leukemia with inv (16) and t (8;21): a Cancer and Leukemia Group B Study. J Clin Oncol. 2006, 24: 3904-3911.PubMedGoogle Scholar
- Kojima K, Konopleva M, McQueen T, O’Brien S, Plunkett W, Andreeff M: Mdm2 inhibitor Nutlin-3a induces p53-mediated apoptosis by transcription-dependent and transcription-independent mechanisms and may overcome Atm-mediated resistance to fludarabine in chronic lymphocytic leukemia. Blood. 2006, 108: 993-1000.PubMed CentralPubMedGoogle Scholar
- Etchin J, Sanda T, Mansour MR, Kentsis A, Montero J, Le BT, Christie AL, McCauley D, Rodig SJ, Kauffman M, Shacham S, Stone R, Letai A, Kung AL, Thomas Look A: KPT-330 inhibitor of CRM1 (XPO1)-mediated nuclear export has selective anti-leukaemic activity in preclinical models of T-cell acute lymphoblastic leukaemia and acute myeloid leukaemia. Br J Haematol. 2013, 161: 117-127.PubMed CentralPubMedGoogle Scholar
- Pui CH, Evans WE: Treatment of acute lymphoblastic leukemia. N Engl J Med. 2006, 354: 166-178.PubMedGoogle Scholar
- Pui CH, Robison LL, Look AT: Acute lymphoblastic leukaemia. Lancet. 2008, 371: 1030-1043.PubMedGoogle Scholar
- Zhao Y, Huang H, Wei G: Novel agents and biomarkers for acute lymphoid leukemia. J Hematol Oncol. 2013, 6: 40-PubMed CentralPubMedGoogle Scholar
- Farnsworth P, Ward D, Reddy V: Persistent complete molecular remission after nilotinib and graft-versus-leukemia effect in an acute lymphoblastic leukemia patient with cytogenetic relapse after allogeneic stem cell transplantation. Exp Hematol Oncol. 2012, 1: 29-PubMed CentralPubMedGoogle Scholar
- Nagafuji K, Miyamoto T, Eto T, Kamimura T, Taniguchi S, Okamura T, Ohtsuka E, Yoshida T, Higuchi M, Yoshimoto G, Fujisaki T, Abe Y, Takamatsu Y, Yokota S, Akashi K, Harada M: Monitoring of minimal residual disease (MRD) is useful to predict prognosis of adult patients with Ph-negative ALL: results of a prospective study (ALL MRD2002 Study). J Hematol Oncol. 2013, 6: 14-PubMed CentralPubMedGoogle Scholar
- Walker CJ, Oaks JJ, Santhanam R, Neviani P, Harb JG, Ferenchak G, Ellis JJ, Landesman Y, Eisfeld AK, Gabrail NY, Smith CL, Caligiuri MA, Hokland P, Roy DC, Reid A, Milojkovic D, Goldman JM, Apperley J, Garzon R, Marcucci G, Shacham S, Kauffman MG, Perrotti D: Preclinical and clinical efficacy of XPO1/CRM1 inhibition by the karyopherin inhibitor KPT-330 in Ph + leukemias. Blood. 2013, 122: 3034-3044.PubMed CentralPubMedGoogle Scholar
- Yee KWL, Savona M, Sorensen M, Brown P, Blum WG, DeAngelo DJ, Gutierrez M, Garzon R, Schuh AC, Gabrail NY, Wadleigh M, Lancet JE, Shah BD, Berdeja JG, Wagner-Johnston ND, Flinn I, Rashal T, Kauffman M, Shacham S, Stone RM: A phase 1 dose-escalation study of the oral selective inhibitor of nuclear export (SINE) KPT-330 (selinexor) in patients (pts) with relapsed/refractory acute myeloid leukemia (AML). ASCO Meeting Abstracts. 2014, 32: 7032-Google Scholar
- Akinleye A, Avvaru P, Furqan M, Song Y, Liu D: Phosphatidylinositol 3-kinase (PI3K) inhibitors as cancer therapeutics. J Hematol Oncol. 2013, 6: 88-PubMed CentralPubMedGoogle Scholar
- Akinleye A, Chen Y, Mukhi N, Song Y, Liu D: Ibrutinib and novel BTK inhibitors in clinical development. J Hematol Oncol. 2013, 6: 59-PubMed CentralPubMedGoogle Scholar
- Lu K, Wang X: Therapeutic advancement of chronic lymphocytic leukemia. J Hematol Oncol. 2012, 5: 55-PubMed CentralPubMedGoogle Scholar
- Wu M, Akinleye A, Zhu X: Novel agents for chronic lymphocytic leukemia. J Hematol Oncol. 2013, 6: 36-PubMed CentralPubMedGoogle Scholar
- Cang S, Mukhi N, Wang K, Liu D: Novel CD20 monoclonal antibodies for lymphoma therapy. J Hematol Oncol. 2012, 5: 64-PubMed CentralPubMedGoogle Scholar
- Wang K, Wei G, Liu D: CD19: a biomarker for B cell development, lymphoma diagnosis and therapy. Exp Hematol Oncol. 2012, 1: 36-PubMed CentralPubMedGoogle Scholar
- Han E, Li X-L, Wang C-R, Li T-F, Han S-Y: Chimeric antigen receptor-engineered T cells for cancer immunotherapy: progress and challenges. J Hematol Oncol. 2013, 6: 47-PubMed CentralPubMedGoogle Scholar
- Chen Y, Liu D: Chimeric antigen receptor (CAR)-directed adoptive immunotherapy: a new era in targeted cancer therapy. Stem Cell Invest. 2014, 1: 2-Google Scholar
- Furman RR, Asgary Z, Mascarenhas JO, Liou HC, Schattner EJ: Modulation of NF-kappa B activity and apoptosis in chronic lymphocytic leukemia B cells. J Immunol. 2000, 164: 2200-2206.PubMedGoogle Scholar
- Reed JC, Pellecchia M: Apoptosis-based therapies for hematologic malignancies. Blood. 2005, 106: 408-418.PubMedGoogle Scholar
- Schmidt J, Braggio E, Kortuem KM, Egan JB, Zhu YX, Xin CS, Tiedemann RE, Palmer SE, Garbitt VM, McCauley D, Kauffman M, Shacham S, Chesi M, Bergsagel PL, Stewart AK: Genome-wide studies in multiple myeloma identify XPO1/CRM1 as a critical target validated using the selective nuclear export inhibitor KPT-276. Leukemia. 2013, 27: 2357-2365.PubMed CentralPubMedGoogle Scholar
- Grinberg AV, Hu CD, Kerppola TK: Visualization of Myc/Max/Mad family dimers and the competition for dimerization in living cells. Mol Cell Biol. 2004, 24: 4294-4308.PubMed CentralPubMedGoogle Scholar
- Chng WJ, Huang GF, Chung TH, Ng SB, Gonzalez-Paz N, Troska-Price T, Mulligan G, Chesi M, Bergsagel PL, Fonseca R: Clinical and biological implications of MYC activation: a common difference between MGUS and newly diagnosed multiple myeloma. Leukemia. 2011, 25: 1026-1035.PubMed CentralPubMedGoogle Scholar
- Golomb L, Bublik DR, Wilder S, Nevo R, Kiss V, Grabusic K, Volarevic S, Oren M: Importin 7 and exportin 1 link c-Myc and p53 to regulation of ribosomal biogenesis. Mol Cell. 2012, 45: 222-232.PubMed CentralPubMedGoogle Scholar
- Delmore JE, Issa GC, Lemieux ME, Rahl PB, Shi J, Jacobs HM, Kastritis E, Gilpatrick T, Paranal RM, Qi J, Hesi M, Schinzel AC, McKeown MR, Heffernan TP, Vakoc CR, Bergsagel PL, Ghobrial IM, Richardson PG, Young RA, Hahn WC, Anderson KC, Kung AL, Bradner JE, Mitsiades CS: BET bromodomain inhibition as a therapeutic strategy to target c-Myc. Cell. 2011, 146: 904-917.PubMed CentralPubMedGoogle Scholar
- Mochizuki K, Nishiyama A, Jang MK, Dey A, Ghosh A, Tamura T, Natsume H, Yao H, Ozato K: The bromodomain protein Brd4 stimulates G1 gene transcription and promotes progression to S phase. J Biol Chem. 2008, 283: 9040-9048.PubMed CentralPubMedGoogle Scholar
- Fang X, Zhou X, Wang X: Clinical development of phosphatidylinositol 3-kinase inhibitors for non-Hodgkin lymphoma. Biomarker Res. 2013, 1: 30-Google Scholar
- Novero A, Ravella P, Chen Y, Dous G, Liu D: Ibrutinib for B cell malignancies. Exp Hematol Oncol. 2014, 3: 4-PubMed CentralPubMedGoogle Scholar
- Zhao X, Zhang W, Wang L, Zhao W-L: Genetic methylation and lymphoid malignancies: biomarkers of tumor progression and targeted therapy. Biomarker Res. 2013, 1: 24-Google Scholar
- Fischer K, Cramer P, Busch R, Stilgenbauer S, Bahlo J, Schweighofer CD, Bottcher S, Staib P, Kiehl M, Eckart MJ, Kranz G, Goede V, Elter T, Buhler A, Winkler D, Kneba M, Dohner H, Eichhorst BF, Hallek M, Wendtner CM: Bendamustine combined with rituximab in patients with relapsed and/or refractory chronic lymphocytic leukemia: a multicenter phase II trial of the German Chronic Lymphocytic Leukemia Study Group. J Clin Oncol. 2011, 29: 3559-3566.PubMedGoogle Scholar
- Wierda WG, Padmanabhan S, Chan GW, Gupta IV, Lisby S, Osterborg A: Ofatumumab is active in patients with fludarabine-refractory CLL irrespective of prior rituximab: results from the phase 2 international study. Blood. 2011, 118: 5126-5129.PubMedGoogle Scholar
- Yoshimura M, Ishizawa J, Ruvolo V, Dilip A, Quintás-Cardama A, McDonnell TJ, Neelapu SS, Kwak LW, Shacham S, Kauffman M, Tabe Y, Yokoo M, Kimura S, Andreeff M, Kojima K: Induction of p53-mediated transcription and apoptosis by exportin-1 (XPO1) inhibition in mantle cell lymphoma. Cancer Sci. 2014, 105 (7): 795-801.PubMed CentralPubMedGoogle Scholar
- Zhang K, Wang M, Tamayo AT, Shacham S, Kauffman M, Lee J, Zhang L, Ou Z, Li C, Sun L, Ford RJ, Pham LV: Novel selective inhibitors of nuclear export CRM1 antagonists for therapy in mantle cell lymphoma. Exp Hematol. 2013, 41: 67-78. e64PubMedGoogle Scholar
- London CA, Bernabe LF, Barnard S, Kisseberth WC, Borgatti A, Henson M, Wilson H, Jensen K, Ito D, Modiano JF, Bear MD, Pennell ML, Saint-Martin JR, McCauley D, Kauffman M, Shacham S: Preclinical evaluation of the novel, orally bioavailable Selective Inhibitor of Nuclear Export (SINE) KPT-335 in spontaneous canine cancer: results of a phase I study. PLoS One. 2014, 9: e87585-PubMed CentralPubMedGoogle Scholar
- Gutierrez M, Goy A, Byrd JC, Flynn JM, Sorensen M, Brown P, Gabrail NY, Savona M, Flinn I, Baz RC, Shah BD, Stone RM, Jacobsen E, Kukreti V, Tiedemann RE, Rashal T, Mirza MR, Shacham S, Kauffman M, Kuruvilla J: A phase 1 dose-escalation study of the oral selective inhibitor of nuclear export (SINE) KPT-330 (selinexor) in patients (pts) with heavily pretreated non-Hodgkin lymphoma (NHL). ASCO Meeting Abstracts. 2014, 32: 8518-Google Scholar
- WeiSZ L, Efferth T: Polo-like kinase 1 as target for cancer therapy. Exp Hematol Oncol. 2012, 1: 38-Google Scholar
- Wang ML, Rule S, Martin P, Goy A, Auer R, Kahl BS, Jurczak W, Advani RH, Romaguera JE, Williams ME, Barrientos JC, Chmielowska E, Radford J, Stilgenbauer S, Dreyling M, Jedrzejczak WW, Johnson P, Spurgeon SE, Li L, Zhang L, Newberry K, Ou Z, Cheng N, Fang B, McGreivy J, Clow F, Buggy JJ, Chang BY, Beaupre DM, Kunkel LA: Targeting BTK with ibrutinib in relapsed or refractory mantle-cell lymphoma. N Engl J Med. 2013, 369: 507-516.PubMed CentralPubMedGoogle Scholar
- Wiestner A: Targeting B-Cell receptor signaling for anticancer therapy: the Bruton's tyrosine kinase inhibitor ibrutinib induces impressive responses in B-cell malignancies. J Clin Oncol. 2013, 31: 128-130.PubMedGoogle Scholar
- Woyach JA, Johnson AJ, Byrd JC: The B-cell receptor signaling pathway as a therapeutic target in CLL. Blood. 2012, 120: 1175-1184.PubMed CentralPubMedGoogle Scholar
- Zucca E, Bertoni F: Toward new treatments for mantle-cell lymphoma?. N Engl J Med. 2013, 369: 571-572.PubMedGoogle Scholar
- Gerecitano J: SINE (selective inhibitor of nuclear export) - translational science in a new class of anti-cancer agents. J Hematol Oncol. 2014, 7: 67-PubMed CentralPubMedGoogle Scholar
- Gravina G, Tortoreto M, Mancini A, Addis A, Di Cesare E, Lenzi A, Landesman Y, McCauley D, Kauffman M, Shacham S, Zaffaroni N, Festuccia C: XPO1/CRM1-Selective Inhibitors of Nuclear Export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa). J Hematol Oncol. 2014, 7: 46-PubMed CentralPubMedGoogle Scholar
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