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STUMP un“stumped”: anti-tumor response to anaplastic lymphoma kinase (ALK) inhibitor based targeted therapy in uterine inflammatory myofibroblastic tumor with myxoid features harboring DCTN1-ALK fusion
Journal of Hematology & Oncology volume 8, Article number: 66 (2015)
Recurrent, metastatic mesenchymal myxoid tumors of the gynecologic tract present a management challenge as there is minimal evidence to guide systemic therapy. Such tumors also present a diagnostic dilemma, as myxoid features are observed in leiomyosarcomas, inflammatory myofibroblastic tumors (IMT), and mesenchymal myxoid tumors. Comprehensive genomic profiling was performed in the course of clinical care on a case of a recurrent, metastatic myxoid uterine malignancy (initially diagnosed as smooth muscle tumor of uncertain malignant potential (STUMP)), to guide identify targeted therapeutic options. To our knowledge, this case represents the first report of clinical response to targeted therapy in a tumor harboring a DCTN1-ALK fusion protein.
Hybridization capture of 315 cancer-related genes plus introns from 28 genes often rearranged or altered in cancer was applied to >50 ng of DNA extracted from this sample and sequenced to high, uniform coverage. Therapy was given in the context of a phase I clinical trial ClinicalTrials.gov Identifier: (NCT01548144).
Immunostains showed diffuse positivity for ALK1 expression and comprehensive genomic profiling identified an in frame DCTN1-ALK gene fusion. The diagnosis of STUMP was revised to that of an IMT with myxoid features. The patient was enrolled in a clinical trial and treated with an anaplastic lymphoma kinase (ALK) inhibitor (crizotinib/Xalkori®) and a multikinase VEGF inhibitor (pazopanib/Votrient®). The patient experienced an ongoing partial response (6+ months) by response evaluation criteria in solid tumors (RECIST) 1.1 criteria.
For myxoid tumors of the gynecologic tract, comprehensive genomic profiling can identify clinical relevant genomic alterations that both direct treatment targeted therapy and help discriminate between similar diagnostic entities.
Myxoid neoplasms of the uterus are a diverse group of soft tissue tumors presenting diagnostic dilemmas for pathologists . The mainstay of treatment for uterine myxoid neoplasms is surgical resection. However, recurrent and metastatic myxoid neoplasms are challenging to manage medically due to scant evidence-based guidance. The combination of a challenging diagnostic proposition and only limited evidence to guide management is a strong rationale for the use of comprehensive genomic profiling (CGP) in an effort to uncover clinically relevant genomic alterations (CRGA) that suggest possible benefit from targeted therapy. Here, we report the case of a patient with a recurrent, metastatic uterine myxoid neoplasm staining diffusely for ALK1 and harboring a DCTN1-ALK fusion identified by CGP who has experienced clinical and radiographic improvement with targeted inhibition of anaplastic lymphoma kinase (ALK) (crizotibib/Xalkori®) and additional targeted therapy (pazopanib/Votrient®).
Patients and methods
Patient selection and clinical assessments
The team reviewed the medical records of a patient who presented to the Department of Investigational Cancer Therapeutics at The University of Texas MD Anderson Cancer Center following an initial diagnosis of a myxoid uterine neoplasm. With minimal standard of care options left, the patient was advised to participate in a clinical trial. Treatment and consent on the investigational trial and data collection were performed in accordance with the guidelines of The University of Texas MD Anderson Cancer Center Institutional Review Board (IRB). Tumor response was determined using response evaluation criteria in solid tumors (RECIST) (version 1.1) by CT scan obtained every 2 cycles post treatment initiation. Clinical evaluation and assessments were performed per protocol.
Comprehensive genomic profiling was performed using the FoundationOne® assay in a Clinical Laboratory Improvement Amendments (CLIA)-certified, CAP-accredited central laboratory (Foundation Medicine, Cambridge, MA, USA). Hybridization capture of 315 cancer-related genes plus introns from 28 genes often rearranged or altered in cancer was applied to >50 ng of DNA extracted from this sample and sequenced to high, uniform coverage. All classes of genomic alterations, including base substitutions, small insertions and deletions (indels), rearrangements, and copy number alterations, were assessed. Clinically relevant genomic alterations (CRGA) were defined as those suggesting benefit from an approved targeted therapy or directing benefit from mechanism-based clinical trials.
Results and discussion
A female in her 50’s Gravida 0, with a long standing history of gynecologic discomfort with history of laparoscopy and hysteroscopy that showed endometriosis and uterine fibroids presented to the clinic with increasing pelvic pressure sensations and significant cramps, symptoms concerning for an abdomino-pelvic neoplasm. At presentation, her disease was described as a 14–16-week sized globular intra-uterine mass and clinically diagnosed as a leiomyoma. Morcellation was performed, and pathologic examination of the formalin fixed paraffin embedded (FFPE) morcellated tissue revealed a myxoid neoplasm, consistent with a smooth muscle tumor of uncertain malignant potential (STUMP). This pathologic diagnosis was done at the outside institution. The patient was subsequently symptomatically monitored for disease progression. Eight months following diagnosis, the patient reported pelvic pain and underwent a bilateral salpingo-oophorectomy, pelvic lymphadenectomy, and omentectomy. Pathologic examination confirmed metastatic myxoid neoplasm within the pelvis right wall, peritoneum, bladder, and peritoneal cul-de-sac. The patient was again monitored, and 7 months later, follow-up imaging identified a 2-cm mass abutting the right external iliac artery. A laparoscopic procedure was performed and confirmed a recurrence of myxoid tumor. The patient was followed for 2 years in which disease subsequently recurred as a lesion in the liver, multiple vaginal tumors, and recurrent tumor over the external iliac artery. These presumed recurrences were biopsied, confirmed as recurrent disease, and resected. A decision was made to investigate systemic treatment, as local management was not effective.
The patient presented to The University of Texas MD Anderson Cancer Center for therapy recommendations. The patient was seen by the gynecological oncologist, sarcoma medical oncologist, and investigational cancer therapeutics consultant at the clinical center for targeted therapy. The natural history of rapid recurrences after initial local management was clearly inconsistent with a typical STUMP. The specimens were requested for pathology confirmation.
The diagnostic specimen was immunostained and demonstrated diffuse positivity for ALK as well as positivity for desmin (Fig. 1). At the same time, this specimen was submitted as an FFPE block for comprehensive genomic profiling .
Three hundred fifteen cancer-related genes plus select introns from 28 genes often rearranged or altered in solid tumor cancers were sequenced to a median depth of coverage of 481×. A deletion event on chromosome 2 (Fig. 2), giving rise to a DCTN1-ALK fusion (Fig. 3), was identified. No other genomic alterations were identified in the specimen.
Based on the diagnostic results of diffuse ALK expression indicating an alteration of ALK, the diagnosis was updated to that of an inflammatory myofibroblastic tumor (IMT) with myxoid features. The comprehensive genomic profiling confirmed the diagnosis by demonstrating a DCTN1-ALK fusion.
The patient was presented in the targeted therapy clinical trials treatment planning meeting, and treatment with an ALK inhibitor was recommended. At this time, the patient returned to the Department of Investigational Cancer Therapeutics at The University of Texas MD Anderson Cancer Center for further treatment discussions including a targeted therapy consult for ALK inhibitor options. The patient was enrolled in a phase I clinical trial (ClinicalTrials.gov Identifier: NCT01548144) of crizotinib in combination with pazopanib for the treatment of advanced cancer . The patient was treated with crizotinib 250 mg orally on alternating days and pazopanib 200 mg orally daily for a 21 day cycle. After 2 cycles of therapy, the patient had greater than 30 % reduction in the sum of longest diameter (SLD) of target lesions per RECIST 1.1 (Fig. 4), indicating a partial response to therapy. The patient tolerated the combination therapy well. She had grade 1 mild diarrhea which was controlled by loperamide and grade 1 nausea which was controlled by ondansetron. At the time of submission, the patient continues to have a response for over 6 months with significant decrease in the tumor measurements and is in confirmed partial remission (PR).
Given the revised diagnosis in this case, we wondered if other advanced uterine neoplasms harbored ALK aberrations and rearrangements. A large series of comprehensive genomic profiles of advanced uterine leiomyosarcomas (LMS) obtained in the course of clinical care were analyzed. Of 139 LMS cases, 5 ALK rearrangements were identified for a frequency of 3.6 % (Elvin et al., manuscript in preparation, personal communication).
ALK inhibitors are the standard of care for ALK-rearranged non-small cell lung cancer, and the resulting success is the paradigm for precision oncology . This case harbors a DCTN1-ALK fusion which has been previously reported [5, 6] but is described here in a myxoid uterine neoplasm for the first time. After multiple recurrences, ALK immunohistochemistry (IHC) was performed concurrently with comprehensive genomic profiling (CGP) on the diagnostic specimen. Based on the tumor harboring an ALK fusion and the staining for ALK1 by immunohistochemistry, the diagnosis was changed to an inflammatory myofibroblastic tumor (IMT) with myxoid features. Treatment with a combination of crizotinib and pazopanib was initiated in the context of a clinical trial and yielded an ongoing partial response for this case.
ALK rearrangements resulting in an activated ALK fusion protein were first identified in 1994 with the observation of nucelophosmin (NPM1)-ALK in anaplastic large cell lymphoma . More recently, ALK rearrangements have been implicated in a variety of tumor types, notably non-small cell lung carcinoma (NSCLC), inflammatory myofibroblastic tumors (IMT), and renal cancer . Particularly in NSCLC, the presence of an ALK fusion predicts response to crizotinib . The rearrangement observed in this case, DCTN1-ALK, has been previously reported in a pediatric case of IMT in the soft tissue of the neck  and 6/140 atypical Spitz tumors, the latter as identified by CGP .
The most common mechanism of ALK fusions is a genomic rearrangement involving the ALK locus at 2p23 with the common breakpoint in intron 19 resulting in the dissociation of the 3′ exons 20–29 from the 5′ exons 1–19 . The DCTN1-ALK intra-chromosomal rearrangement detected in this case conformed to the common structural organization of ALK fusions, with the vast majority having an ALK intron 19 breakpoint, and is a priori suspected to be active in vivo. The DCTN1 component of the fusion contains exons 1–29 including coiled-coil domains, which is structurally similar to other previously reported ALK fusion partners (Figs. 2 and 3). Via these domains, DCTN1 is suspected to promote dimerization of ALK and subsequent kinase activation by transphosphorylation [5, 8].
Myxoid neoplasms of the uterus are defined by myxoid features but can carry a range of diagnoses [9, 10]. Diagnoses of such tumors rely on clinicopathologic observations to formulate an effective differential diagnosis . From the initial differential, myxoid neoplasms are most often classified by their clinical behavior—benign, locally aggressive, or malignant nature—as well as by an imputed histogenetic origin including myxoid leiomyosarcoma, myxoid liposarcoma, and myxofibrosarcoma among others .
In particular, the differential diagnosis for a myxoid neoplasm of the uterus must include IMT of the uterus, which are known to have myxoid features. IMTs are characterized by myofibroblastic spindle cells accompanied by a lymphoplasmacytic inflammatory infiltrate [12, 13]. The myxoid morphologic appearance of an IMT can create diagnostic challenges, particularly if pauci-inflammatory, and the differential includes benign myxoid leiomyoma and malignant myxoid and inflammatory leiomyosarcoma .
The diagnostic challenge associated with differentiating between uterine myxoid neoplasms and uterine IMT can be resolved through the identification of kinase fusion events. Previous data have demonstrated that approximately 50 % of IMT cases are ALK positive [12, 15, 16]. Additional studies have showed that of ALK IHC-negative case, 8/11 (73 %) identified alternate kinase fusions, including 2 cases with ALK fusions missed by ALK IHC and 6 cases harboring either ROS1 (4) or PDGFRβ (2) fusions . The diagnosis of an IMT with uterine origin is further compounded by its rarity and nonspecific symptoms; thus, this diagnosis is commonly overlooked by clinicians [14, 17]. To date, ALK positive uterine IMTs have been reported using immunohistochemistry and FISH methods, with previous studies showing 100 % [11, 18] and 87.5 %  ALK positivity in female genital tract IMTs, respectively. However, such studies are limited by the diagnostic criteria used to identify IMTs. If an IMT is in the differential diagnosis for a uterine neoplasm, CGP can identify genomic rearrangements of non-ALK kinases, which will strongly support the diagnosis of an IMT as seen in IMT of soft tissue . Knowledge of the natural history of IMT, both of uterine origin and other, is scant. From IMTs located in the lung, 3- and 5-year survival were 82 and 74 %, respectively, with 15 % of patients experiencing recurrent disease . No clinical trials for systemic treatment have focused solely on IMTs, but a recent phase I trial for refractory pediatric solid tumors demonstrated three of seven ALK-rearranged IMT patients had partial responses, and another three had stable disease on crizotinib treatment .
Crizotinib is an oral small-molecule tyrosine kinase inhibitor that targets the ALK, MET, and ROS1 tyrosine kinases and is well known to induce clinically significant responses in NSCLC [22–24]. Response to crizotinib in IMT harboring ALK fusions have also been observed in two patients [21, 25, 26]. In this case report, we describe the documented clinical response of a uterine IMT to a combination of crizotinib and pazopanib. The question remains if response, toxicity, or overall survival benefitted from combination therapy of crizotinib and pazopanib compared with crizotinib monotherapy. As exemplified by trials for BRAF V600E metastatic melanoma, treatment with dabrafenib monotherapy versus the combination of dabrafenib and trametinib in BRAF V600E mutated melanoma  can significantly improve overall survival without increasing overall toxicities. Crizotinib has been shown to be superior to standard chemotherapy in advanced non-small-cell lung cancer  and is approved as the first line therapy for ALK-rearranged metastatic NSCLC.
However, acquired resistance to crizotinib monotherapy is well demonstrated in the setting of NSCLC, and a similar phenomenon may arise in ALK-rearranged IMT treated with crizotinib . Pazopanib is a multikinase inhibitor with activity against VEGFR1-3, PDGFRA and PDGFRB, FGFR1 and FGFR3, and c-Kit . Whether the combination of crizotinib or pazopanib is synergistic, additive, or deleterious is unknown, and the continuing outcome of the patient may anecdotally address this issue. Arguably, pazopanib is likely to further inhibit signaling downstream of the above receptor tyrosine kinases . This depression of signaling may further benefit the case by inhibiting pathways of acquired resistance akin to PI3K inhibitors and crizotinib in ALK-rearranged NSCLC but would need to be explored rigorously in vitro to prove this possibility.
This report demonstrates the first documented response of a myxoid tumor of the female genital tract harboring an ALK fusion to combination therapy including ALK-targeted therapy. To our knowledge, this case represents the first report of clinical response to targeted therapy in a tumor harboring a DCTN1-ALK fusion protein. This report not only demonstrates the difficulty in diagnosing rare tumors in uterine soft tissue but also emphasizes the use of comprehensive genomic profiling for identifying clinically relevant genomic alterations, such as ALK rearrangements, and immediate implications for patient benefit.
DCTN1-ALK fusion, previously reported in an inflammatory myofibroblastic tumor (IMT) and Spitz tumors, is described here in a myxoid neoplasm of the female reproductive tract.
This case is the first report of a tumor harboring DCTN1-ALK being treated with combination targeted therapy with a documented clinical and radiographic response.
Comprehensively genomic profiling can uncover clinically relevant genomic alterations in rare tumors, such as myxoid uterine neoplasms, that present diagnostic challenges to pathologic and while simultaneously providing unanticipated pathways to benefit from targeted therapy.
Written informed consent was obtained from the patient for enrolling on the clinical trial and for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
anaplastic lymphoma kinase
inflammatory myofibroblastic tumors
smooth muscle tumor of uncertain malignant potential
comprehensive genomic profiling
clinically relevant genomic alterations
Clinical Laboratory Improvement Amendments
formalin fixed paraffin embedded
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The authors thank Pfizer® for providing Crizotinib for the clinical trial. The University of Texas MD Anderson Cancer Center is supported by the National Institutes of Health Cancer Center Support Grant CA016672.
CM, JAE, TB, ZC, JM, VAM, PJS, JC, JSR, and SMA are all employees of Foundation Medicine and have equity interest in Foundation Medicine.
All authors contributed to the manuscript. VS, CM, and SMA conceived the manuscript. VS, SP, JB and CFL provided clinical expertise. RZ is the primary investigator for the clinical trial, and VS is the co-primary investigator for the clinical trial. CO was the clinical coordinator for the clinical trial. VS, CM, SP, SE, JAE, TB, ZRC, JM, VAM, IMS, PJS, JC, JSR, and SMA analyzed the data. DG and DA provided the radiology expertise. SE and SMA provided pathology expertise. VS, CM, IMS, and SMA wrote the paper. VS and SP provided sarcoma expertise. VS and SMA provided cellular, molecular, and targeted therapy expertise. All authors read and approved the final manuscript.
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Subbiah, V., McMahon, C., Patel, S. et al. STUMP un“stumped”: anti-tumor response to anaplastic lymphoma kinase (ALK) inhibitor based targeted therapy in uterine inflammatory myofibroblastic tumor with myxoid features harboring DCTN1-ALK fusion. J Hematol Oncol 8, 66 (2015). https://doi.org/10.1186/s13045-015-0160-2
- Inflammatory myofibroblastic tumor
- Targeted therapy
- ALK fusions
- Uterine myxoid tumors
- Smooth muscle tumor of uncertain malignant potential (STUMP)