- Letter to the Editor
- Open Access
Genetic dynamics in untreated CLL patients with either stable or progressive disease: a longitudinal study
Journal of Hematology & Oncology volume 12, Article number: 114 (2019)
Clonal evolution of chronic lymphocytic leukemia (CLL) often follows chemotherapy and is associated with adverse outcome, but also occurs in untreated patients, in which case its predictive role is debated. We investigated whether the selection and expansion of CLL clone(s) precede an aggressive disease shift. We found that clonal evolution occurs in all CLL patients, irrespective of the clinical outcome, but is faster during disease progression. In particular, changes in the frequency of nucleotide variants (NVs) in specific CLL-related genes may represent an indicator of poor clinical outcome.
To the Editor
In chronic lymphocytic leukemia (CLL), the clonal expansion acquired relevance with the NGS era, which allowed its use for clinical monitoring. Research was mainly performed on large CLL cohorts sampled before and after therapy  and only a few studies investigated clonal evolution longitudinally in stable versus progressive untreated patients [2,3,4]. The key results indicate expansion of specific clones upon therapy and heterogeneity of mutated genes among patients, but the extent to which the genetic dynamics differs between stable and progressive untreated CLLs is still controversial.
To address this point, we used a CLL cohort including untreated sequential samples from patients with either progressive (P-CLL) or stable (S-CLL) disease. Patients’ features are in Additional file 1: Table S1. At each time point, the diagnosis of stable or progressive CLL was established by the clinicians according to the criteria defined during the International Workshop on Chronic Lymphocytic Leukemia . Using genome-wide copy number variation (CNV) analysis, we investigated copy number fluctuations in 11 stable CLLs (S-CLLs) and 15 progressive CLLs (P-CLLs). Data were processed using the Rawcopy package , and paired segments were defined for each patient (Additional file 2: Figure S1). Since the percentage of CLL cells (f) in PBMCs was not always known, analyses were performed varying f from 1 to 100%. To define aberrant loci, we used two sets of thresholds on log ratio (LogR) value, depending on f and on copy number (k) in CLL cells (Additional file 2: Figure S2). We did not find significant differences in percentages of aberrant loci between S-CLLs and P-CLLs (Fig. 1a), but the rate of change (or slope), reflecting the rate of aberrant clones evolving over time, was significantly higher in P-CLLs (p ≤ 0.05, Mann-Whitney U test) (Fig. 1b). Thus, S-CLLs and P-CLLs seemed to have the same probability of acquiring or losing clones, but this phenomenon was faster in P-CLLs. The results were validated on 6 S-CLLs and 5 P-CLLs with known percentage of CLL cells in PBMCs (Additional file 2: Figure S3-S4), suggesting that tracking copy number changes does not mandatorily require knowledge of cancer cell percentage.
To identify genetic events associated with faster clonal expansion, we characterized the CLL-specific genetic features of our cohort. Analyses by qPCR of three chromosomal abnormalities of prognostic value, del (11q), tri (12), and del (17p) , did not reveal significant differences between S-CLLs and P-CLLs (Additional file 2: Figure S5). Subsequently, we characterized 11 S-CLLs and 17 P-CLLs for point mutations or indels in regions of 27 genes reported as mutated in CLL (Additional file 3: Table S2). We did not register any significant difference between S-CLLs and P-CLLs with regard to frequency and number of nucleotide variants (NVs) (data not shown). Next, we focused only on NVs with variant allele frequencies (VAF) changing more than 20% between longitudinal samples (dynamic NV: dNVs, synonymous or non-synonymous). We detected on average 1.18 and 3.35 dNVs per sample in S-CLLs and P-CLLs, respectively (Additional file 4: Table S3). P-CLLs showed higher gains/increases of dNVs (p = 0.0008, Fisher’s test) (Fig. 2a). Patients with dNV > 1 had shorter treatment-free survival (TFS), considering as starting point the date at first sampling (p = 0.0029) or at diagnosis (p = 0.0004, log rank test) (Fig. 2b and Additional file 2: Figure S6). A dNV > 1 was also associated with poor prognostic factors, including unmutated IGVH and trisomy 12 (p = 0.0461 and p = 0.0407, respectively, Fisher’s test) (Additional file 5: Table S4). Patients with unmutated IGVH showed shorter TFS, supporting the reliability of our cohort (Fig. 2b). Finally, we found that in P-CLLs the average of dNV frequencies was higher in the first sample (p = 0.0074, Mann-Whitney U test), where it was not associated with IGVH mutational status (Fig. 2c). These findings suggest that dNVs could have an exploitable clinical relevance. However, since dNVs include synonymous/non-synonymous mutations and NVs in non-coding regions, we cannot speculate on the molecular role of the targeted genes most frequently mutated, such as ITPKB and NOTCH1 (Fig. 2a). Indeed, these dNVs were only used here to track genetic evolution.
In conclusion, differently from previous studies, we calculated VAFs on PBMCs, demonstrating that this is reliable to track CLL evolution. In fact, an increase of a single VAF over time indicates expansion of the clone carrying that NV, regardless of variation in cancer cell fraction. Overall, our study points to a higher genetic dynamics in P-CLLs and suggests that monitoring VAFs of a specific gene panel in PBMCs from sequential samples of a CLL patient may predict disease progression.
Availability of data and materials
DNA CNVs and mutational data are freely available to ArrayExpress database (accession number E-MTAB-8020) and European Nucleotide Archive database (accession number ERP115524). All the other raw data are freely available at the code-hosting platform GitHub (https://github.com/VeroneseVisoneLabs/Genetic-dynamics-in-untreated-CLL-patients-with-either-stable-or-progressive-disease-a-longitudinal).
Chronic lymphocytic leukemia
Copy number variation
Dynamic nucleotide variant
First time point
Last time point
Peripheral blood mononuclear cell
Progressive chronic lymphocytic leukemia
Stable chronic lymphocytic leukemia
Variant allele frequency
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We thank Ms. Lia De Amicis for the administrative work. We thank Valerie Matarese for the manuscript editing and Prof. Carlo Maria Croce for providing DNA samples.
This study is supported by the Italian Association for Cancer Research (AIRC) with Start Up grant 2010 (10054) to RV and partially by the Italian Association for Cancer Research (AIRC IG-17063) to SV. RV was supported by her own Marie Curie Career Integration Grant (GA-2011-303735).
Ethics approval and consent to participate
The institutional review board of the University of California, San Diego (171884CX), and of the Fondazione Policlinico Agostino Gemelli (P/948/CE/2011) approved the research protocol. Samples were provided upon written informed consent.
Consent for publication
The authors declare that they have no competing interests.
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Additional file 1: Table S1
Molecular and clinical features of all patients included in the study.
Additional file 2.
Additional file 3: Table S2
HaloPlex SureDesign Report.
Additional file 4: Table S3
Genetic variants represented in Fig. 2.
Additional file 5: Table S4.
Characteristics of patients having few (dNV≤1) versus many (dNV>1) nucleotide variants.
Additional file 6: Table S6.
SNPs and mutations described by dbSNP and COSMIC databases, respectively, and localized in the regions analyzed by Haloplex SureDesign.
Additional file 7: Table S7.
SNPs and mutations detected by Haloplex SureDesign Panel.
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Ramassone, A., D’Argenio, A., Veronese, A. et al. Genetic dynamics in untreated CLL patients with either stable or progressive disease: a longitudinal study. J Hematol Oncol 12, 114 (2019). https://doi.org/10.1186/s13045-019-0802-x
- Chronic lymphocytic leukemia
- Copy number variation
- Nucleotide variation
- Clonal evolution