XPO1 expression worsens the prognosis of unfavorable DLBCL that can be effectively targeted by selinexor in the absence of mutant p53

Abstract

The XPO1 inhibitor selinexor was recently approved in relapsed/refractory DLBCL patients but only demonstrated modest anti-DLBCL efficacy, prompting us to investigate the prognostic effect of XPO1 in DLBCL patients and the rational combination therapies in high-risk DLBCL. High XPO1 expression (XPO1^high) showed significant adverse prognostic impact in 544 studied DLBCL patients, especially in those with BCL2 overexpression. Therapeutic study in 30 DLBCL cell lines with various molecular and genetic background found robust cytotoxicity of selinexor, especially in cells with BCL2-rearranged (BCL2-R⁺) DLBCL or high-grade B-cell lymphoma with MYC/BCL2 double-hit (HGBCL-DH). However, expression of mutant (Mut) p53 significantly reduced the cytotoxicity of selinexor in overall cell lines and the BCL2-R and HGBCL-DH subsets, consistent with the favorable impact of XPO1^high observed in Mut-p53-expressing patients. The therapeutic effect of selinexor in HGBCL-DH cells was significantly enhanced when combined with a BET inhibitor INCB057643, overcoming the drug resistance in Mut-p53-expressing cells. Collectively, these data suggest that XPO1 worsens the survival of DLBCL patients with unfavorable prognostic factors such as BCL2 overexpression and double-hit, in line with the higher efficacy of selinexor demonstrated in BCL2-R⁺ DLBCL and HGBCL-DH cell lines. Expression of Mut-p53 confers resistance to selinexor treatment, which can be overcome by combined INCB057643 treatment in HGBCL-DH cells. This study provides insight into the XPO1 significance and selinexor efficacy in DLBCL, important for developing combination therapy for relapsed/refractory DLBCL and HGBCL-DH.

To the editor

XPO1 (exportin 1) is a well-characterized nuclear export protein responsible for the nuclear-cytoplasmic transport and cellular homeostasis of up to 220 cargoes, including the tumor suppressors p53 and IκB [1, 2]. Abnormal XPO1 expression correlates with worse prognoses in human malignancies. Targeting XPO1 is a promising therapeutic approach in cancer [1, 2]. The XPO1 inhibitor selinexor has received FDA approval recently to treat refractory/relapsed (R/R) diffuse large B-cell lymphoma (DLBCL) after at least 2 lines of systemic therapy, showing an overall response rate of 28% in the SADAL trial [3]. However, it remains largely unknown whether and how XPO1 interplays with other adverse predictors in DLBCL, how to predict selinexor effectiveness, and what combination therapy is optimal in R/R DLBCL patients. Here, we evaluated the prognostic significance of XPO1 expression in 544 well-characterized DLBCL cases, and investigated the therapeutic effect of selinexor in 30 DLBCL cell lines with variable genetic background.

Patients and Methods for this study are detailed in Additional file 1. XPO1 expression was observed in 217 of 544 (40%) DLBCL patients with a mean level of 24%. High level of XPO1 expression (XPO1^high; > 30%) predicted significantly poor progressive-free survival (PFS) and overall survival (OS) in DLBCL patients (Fig. 1a). DLBCL is classified into prognostic favorable germinal center B-cell-like (GCB) and unfavorable activated B-cell-like (ABC) subtypes [4]. XPO1^high significantly shortened the PFS/OS in ABC-DLBCL but not GCB-DLBCL (Additional file 1: Figure S1A–B). XPO1^high showed significant association with p53 overexpression (p53⁺) and dual p53⁺MYC^high expression but not clinical features (Additional file 1: Table S1), unlike a previous study using a different scoring system for XPO1 expression in 131 DLBCL patients [5].

Fig. 1

Impact of XPO1 expression on patient survival in DLBCL. a In the entire cohort, DLBCL patients with high level of XPO1 expression (XPO1^high) had significantly worse OS and PFS than those with low or negative XPO1 expression (XPO1^low). b XPO1^high remarkably worsened the OS/PFS of DLBCL patients with BCL2^high expression. c XPO1^high significantly worsened the OS/PFS of patients with dual MYC^highBCL2^high expression, and showed a trend of unfavorable effect on OS in patients with dual MYC/BCL2 rearrangements (MYC-R⁺BCL2-R⁺, HGBCL-DH). d In TP53-mutated (Mut) DLBCL patients without Mut-p53 overexpression, XPO1^high showed a trend of unfavorable prognostic effect on OS. e In Mut-TP53 DLBCL patients with Mut-p53 overexpression, XPO1^high showed favorable prognostic effect, which was not significant in overall patients but significant in the subset with low BCL2 expression. f Significantly differentially expressed genes between XPO1^high and XPO1^low patients with concurrent Mut-TP53 and MYC^high

Whether XPO1^high interacts with other adverse prognostic factors and whether XPO1 is a potential therapeutic target in high-risk DLBCL patients were further examined. XPO1^high remarkably worsened the OS and PFS of DLBCL with BCL2^high or dual MYC^highBCL2^high expression (Fig. 1b,c), which is known as double-expressor lymphoma with unfavorable prognosis [6]. Trends of adverse impact were also observed on PFS in MYC-rearranged (R⁺) patients (P = 0.097; Additional file 1: Figure S1C) and OS in patients with dual MYC-R⁺BCL2-R⁺ (Fig. 1c) with dismal prognosis, defined as high-grade B-cell lymphoma with MYC/BCL2 double-hit (HGBCL-DH) [7]. In patients with TP53 mutation (Mut-TP53) [8], XPO1^high showed opposite prognostic effects in patients with and without Mut-p53 protein overexpression [9], suggesting the nuclear export may attenuate the oncogenic gain-of-function of Mut-p53. In contrast to the negative impact of XPO1^high in Mut-TP53/p53-negative patients (Fig. 1d) and in TP53-wild type (Wt-TP53) patients (Additional file 1: Figure S1D), a favorable effect was associated with XPO1^high in Mut-TP53/p53-positive patients, which was significant in the BCL2^low subset (Fig. 1e). Gene expression profiling [4] analysis identified a distinct gene expression signature for XPO1^high in patients with Mut-TP53 and MYC^high (Fig. 1f), including upregulation of SIRPA, which encodes SIRPα, a receptor for CD47 transmitting “do not eat me” signal in phagocytosis, and downregulation of several genes related to DNA repair, metabolism, splicing, or biosynthesis (Additional file 1: Table S2).

Next, selinexor was assessed in 30 DLBCL cell lines, which resulted in significantly reduced cell viability with varying IC50 values (Fig. 2a). ABC-DLBCL and GCB-DLBCL cells were similarly vulnerable to selinexor (Additional file 1: Figure S1E), consistent with results in the SADAL clinical trial [3]. Biomarkers significantly associated with higher sensitivity (lower IC50) to selinexor cytotoxicity included BCL2-R and HGBCL-DH (Fig. 2b) but not MYC-R. In contrast, presence of Mut-TP53/p53⁺ significantly reduced the anti-lymphoma efficacy of selinexor, especially in HGBCL-DH cells (Fig. 2c; Additional file 1: Figure S1F).

Fig. 2

Therapeutic effect of selinexor alone or in combination with a BET inhibitor INCB057643 in DLBCL cellular models. a The effect of 72-h selinexor exposure on cell viability of 30 DLBCL cell lines. Waterfall graph showed the specific IC50 value of selinexor for each cell line with either ABC or GCB subtype of DLBCL. b DLBCL cell lines with BCL2 rearrangement (BCL2-R) or HGBCL-DH were more sensitive to selinexor with a lower mean IC50 value compared with other cell lines. c The presence of mutant (Mut) p53 in DLBCL cells significantly reduced the cytotoxicity of selinexor, especially significant in HGBCL-DH cell lines. Selinexor promoted more significant apoptosis in Wt-TP53 HGBCL-DH cells than in Mut-TP53/p53-expressing HGBCL-DH cells. d INCB057643 and selinexor were cooperative in reducing cell viability and inducing apoptosis in HGBCL-DH cells with Wt-TP53 or Mut-TP53/p53⁺

Limited efficacy of selinexor in HGBCL with Mut-TP53/p53⁺ calls for combination strategy. Previous studies showed the synergy between selinexor and venetoclax in DLBCL and double-hit lymphoma [10, 11]. However, in the SADAL trial [3], patients with MYC^high (but not BCL2^high) expression had a lower overall response rate than those without. MYC expression can be inhibited by targeting the bromodomain and extra-terminal domain (BET) proteins [12]. We therefore combined selinexor with a novel BET inhibitor INCB057643. Synergistic effect was observed in DLBCL/HGBCL cells, especially in HGBCL-DH cells with Mut-TP53/p53⁺ (Fig. 2d), supporting INCB057643/selinexor combination as a therapeutic option for HGBCL-DH patients.

In summary, this study demonstrates that XPO1^high is a valuable biomarker in DLBCL with unfavorable prognostic factors, predictive of significantly poorer outcomes in ABC-DLBCL, BCL2^high DLBCL, and double-expressor lymphoma but not Mut-p53-expressing DLBCL. Targeting XPO1 with selinexor is similarly effective in GCB-DLBCL and ABC-DLBCL cells, and remarkably effective in BCL2-R⁺ DLBCL and HGBCL cells without Mut-TP53/p53-positivity. In DLBCL/HGBCL cells, Mut-TP53/p53-positive expression predicts resistance to selinexor. INCB057643 synergizes with selinexor in HGBCL-DH cells, overcoming resistance in Mut-TP53/p53-positive HGBCL-DH. These findings warrant future investigation on the role of XPO1, selinexor, and combined BET inhibition in R/R DLBCL and HGBCL-DH.