- Open Access
Clinical and genetic features of pediatric acute lymphoblastic leukemia in Down syndrome in the Nordic countries
- Catarina Lundin1, 2Email author,
- Erik Forestier3,
- Mette Klarskov Andersen4,
- Kirsi Autio5,
- Gisela Barbany6,
- Lucia Cavelier7,
- Irina Golovleva3,
- Sverre Heim8,
- Kristiina Heinonen9,
- Randi Hovland10,
- Johann H Johannsson11,
- Eigil Kjeldsen12,
- Ann Nordgren6,
- Lars Palmqvist13,
- Bertil Johansson1, 2,
- the Swedish Cytogenetic Leukemia Study Group (SCLSG) and
- the NOPHO Leukemia Cytogenetic Study Group (NLCSG)
© Lundin et al.; licensee BioMed Central Ltd. 2014
- Received: 6 March 2014
- Accepted: 8 April 2014
- Published: 11 April 2014
Children with Down syndrome (DS) have an increased risk for acute lymphoblastic leukemia (ALL). Although previous studies have shown that DS-ALL differs clinically and genetically from non-DS-ALL, much remains to be elucidated as regards genetic and prognostic factors in DS-ALL.
To address clinical and genetic differences between DS-ALL and non-DS-ALL and to identify prognostic factors in DS-ALL, we ascertained and reviewed all 128 pediatric DS-ALL diagnosed in the Nordic countries between 1981 and 2010. Their clinical and genetic features were compared with those of the 4,647 B-cell precursor (BCP) ALL cases diagnosed during the same time period.
All 128 DS-ALL were BCP ALL, comprising 2.7% of all such cases. The 5-year event-free survival (EFS) and overall survival (OS) were significantly (P = 0.026 and P = 0.003, respectively) worse for DS-ALL patients with white blood cell counts ≥50 × 109/l. The age distributions varied between the DS and non-DS cases, with age peaks at 2 and 3 years, respectively; none of the DS patients had infant ALL (P = 0.029). The platelet counts were lower in the DS-ALL group (P = 0.005). Abnormal karyotypes were more common in non-DS-ALL (P < 0.0001), and there was a significant difference in the modal number distribution, with only 2% high hyperdiploid DS-ALL cases (P < 0.0001). The 5-year EFS and 5-year OS were significantly worse for DS-ALL (0.574 and 0.691, respectively) compared with non-DS-ALL (0.783 and 0.894, respectively) in the NOPHO ALL-1992/2000 protocols (P < 0.001).
The present study adds further support for genetic and clinical differences between DS-ALL and non-DS-ALL.
- Down syndrome
- Clinical features
- Genetic features
It is well established that children with Down syndrome (DS) have an increased risk of developing acute leukemia, with a relative risk of approximately 20 and a cumulative risk of 2.1% at the age of 5 years [1, 2]. Acute lymphoblastic leukemia (ALL) in DS (DS-ALL) affects 1 in 300 children with this syndrome [2, 3] and several studies have shown that the outcome of DS-ALL is inferior to that of non-DS-ALL [4–6]. In fact, leukemia is one of the most common causes of death in individuals with DS, together with respiratory diseases, congenital circulatory defects, diseases of the digestive system, and Alzheimer’s disease . The poor prognosis of DS-ALL has been attributed to a lower remission rate after induction therapy as well as to a higher degree of treatment-related toxicity [4, 8–11].
In contrast to myeloid leukemia in DS (ML-DS), which is associated with a transient myeloproliferative disorder (TMD), DS-ALL does not display any obvious preleukemic phase [12, 13]. Furthermore, specific genetic changes, such as GATA1 mutations in TMD and ML-DS that would possibly enable early diagnosis and treatment, seem to be rare in DS-ALL , although deregulation of the CRLF2 gene at Xp22.33/Yp11.32 is more prevalent in DS-ALL compared with non-DS-ALL, as are specific mutations in exon 16 of JAK2 at 9p24.1 [15–18]. DS-ALL and non-DS-ALL have been reported to display similar median ages, gender distributions, and white blood cell (WBC) counts . Thus, no clinical features, apart from the DS phenotype, at the time of diagnosis distinguish these two patient groups.
However, there are clear immunophenotypic differences between DS-ALL and non-DS-ALL, with mature B-cell ALL (Burkitt leukemia) and T-cell ALL being exceedingly rare in patients with DS; virtually all DS-ALL cases display a B-cell precursor (BCP) phenotype [11, 13, 19–21]. As regards acquired genetic differences, although single nucleotide polymorphism (SNP) analyses have in general identified similar microdeletions in DS-ALL and non-DS-ALL cases [16, 22–25], DS-ALL and non-DS-ALL are to a large extent cytogenetically distinct – DS-ALL are rarely high hyperdiploid (51–67 chromosomes) and seldom display MLL rearrangements or t(9;22)(q34;q11)/BCR-ABL1[5, 20, 26, 27]. The lack of specific ALL-associated gene fusions precludes risk group assignment and hence treatment stratification based on cytogenetics in most instances of DS-ALL.
In order to address further clinical and genetic differences between DS-ALL and non-DS-ALL and to identify prognostic factors in DS-ALL, we have ascertained and reviewed all pediatric ALL cases included in the Nordic Society of Pediatric Hematology, Oncology (NOPHO) registry 1981–2010.
Between 1981 and 2010, a total of 128 childhood and adolescent (<18 years) DS-ALL cases were diagnosed in the Nordic countries (Denmark, Finland, Iceland, Norway, and Sweden) and registered in the NOPHO database. A subset of the 128 patients (43 cases) was included in a recently reported retrospective, international study on DS-ALL . Prior to 1992, some patients may have escaped registration due to a less developed database, but since 1992 data on clinical, immunophenotypic, and genetic features of all Nordic pediatric ALL cases, at least those <15 years of age, have been entered into the NOPHO register, which hence is all-inclusive and truly population-based for this age group since that time point. All DS patients had BCP ALL, and for this reason their clinical and genetic features were compared only with those of non-DS BCP ALL cases diagnosed during the same time period (n = 4,637). The 128 DS-ALL patients were treated according to five different protocols: NOPHO ALL-1981 (n = 19), NOPHO ALL-1986 (n = 21), NOPHO ALL-1992 (n = 42), NOPHO ALL-2000 (n = 34), and NOPHO ALL-2008 (n = 12). The corresponding distribution of the 4,637 non-DS-ALL patients was NOPHO ALL-1981 (n = 563), NOPHO ALL-1986 (n = 828), NOPHO ALL-1992 (n = 1,554), NOPHO ALL-2000 (n = 1,128), and NOPHO ALL-2008 (n = 564). Details on these protocols have been reported previously [29, 30]. The study was approved by the Regional Ethical Review Board at Lund University and informed consent was obtained according to the Declaration of Helsinki.
Chromosome banding analyses were performed using standard methods in 16 cytogenetic laboratories in the Nordic countries, and all abnormal karyotypes have been centrally reviewed each year since 1996 (Sweden)/2000 (all five Nordic countries) by the Swedish and the NOPHO Leukemia Cytogenetic Study Groups. Fluorescence in situ hybridization (FISH) or reverse-transcription polymerase chain reaction (RT-PCR) analyses were used to screen for the translocations/gene fusions t(1;19)(q23;p13) [TCF3/PBX1], t(9;22)(q34;q11) [BCR/ABL1], and t(12;21)(p13;q22) [ETV6/RUNX1], whereas FISH or Southern blot analyses were used to identify 11q23/MLL rearrangements. These targeted analyses have been performed prospectively from 1996 in Sweden and from 2000 in the other Nordic countries, with several additional cases prior to this time having been analyzed retrospectively. During recent years, various array analyses have also been increasingly used to establish and confirm the registered karyotypes. In the NOPHO ALL-2008 protocol, FISH-based screening for intrachromosomal amplification of chromosome 21 (iAMP21) was added to the targeted analyses. Detailed karyotypic data are given in Additional file 1: Table S1.
The PASW Statistics software for Windows (SPSS Inc., Chicago, IL) was used for all statistical analyses. The significance limit for two-sided P values was set to <0.05. Age, gender, WBC and platelet counts, types of event, and cytogenetic subgroups were compared within the DS-ALL group as well as between the 128 DS-ALL and the 4,637 non-DS-ALL cases using the chi square or the Mann–Whitney U tests with exact calculations of P values. Survival analyses were performed only on cases treated according to the NOPHO ALL-1992 and ALL-2000 protocols, partly because they were almost identical in risk stratification and treatment and partly because they comprised the majority of patients. The probabilities of event-free survival (EFS) and overall survival (OS) were calculated using the Kaplan-Meier method and subgroups were compared using the Log rank test. In the analysis of EFS, events comprised induction failure, relapse, second malignant neoplasm (SMN), and death in complete remission 1 (DCR1). In the OS analyses, death of any cause was the end point. Patients in continuous complete remission 1 were followed between 0 and 355 months (median 134 months).
Within the DS-ALL cohort, there were no significant gender differences in age or WBC count distributions, whereas the platelet counts were higher in girls than in boys (median 47.0 × 109/l, range 5–359, and 26.5 × 109/l, range 4–493, respectively; P = 0.042). Age had no impact on the WBC and platelet counts.
Clinical features of the 128 DS-ALL and the 4,637 non-DS-ALL patients
WBC count (×10 9 /l)
Platelet count (×10 9 /l) d
Genetic features of the DS-ALL and the non-DS-ALL patients
Modal number b
TCF3 - PBX1 c
BCR - ABL1 c
MLL rearrangement c
ETV6 - RUNX1 c
Among the DS-ALL patients, two (1.6%) had trisomy 21 mosaicism (the trisomic cells were involved in the leukemic clone), one (0.8%) had a Robertsonian translocation [der(14;21)(q10;q10)], and one (0.8%) had a complex constitutional unbalanced t(15;21;16) leading to gain of 21q; the remaining 124 (97%) patients had a classical non-mosaic trisomy 21. One patient had both DS and Klinefelter syndrome, i.e., a sex chromosome complement of XXY (Additional file 1: Table S1).
The most common acquired changes in DS-ALL were + X (14% of the 95 informative cases), deletions of 9p (13%; including some cases with microdeletions identified by array analyses), 12p (7%; including some cases with microdeletions identified by FISH and/or array analyses), 13q (6%; the majority identified by array analyses), and 1p (5%), +14/i(14)(q10) (5%), +17/i(17)(q10) (4%), and gain of an extra chromosome 21 (4%). The only recurrent translocations were t(12;21)(p13;q22), detected in 8 (15%) of the 54 cases analyzed by FISH and/or RT-PCR, and t(8;14)(q11;q32), found in two (2%) cases. The ALL-associated translocations t(1;19)(q23;p13) and t(9;22)(q34;q11) [with the P190 BCR/ABL1 transcript] were found in single cases; no DS-ALL harbored an 11q23/MLL rearrangement or an iAMP21. The distributions of modal numbers varied significantly between DS- and non-DS-ALL cases (P < 0.0001), with high hyperdiploidy (51–67 chromosomes) being found in two cases only (Table 2 and Additional file 1: Table S1). Although the frequencies of t(1;19), t(9;22), and t(12;21) were lower in the DS-ALL group, they did not differ significantly from the ones observed in non-DS-ALL. Cases with t(8;14) or iAMP21 were too few to allow meaningful statistical analyses (Table 2).
Survival of the DS-ALL patients in the NOPHO ALL-1992/2000 protocols
Survival of the DS-ALL patients treated according to the ALL-1992/2000 protocols in relation to clinical and genetic features
5-year EFS (SE)
5-year OS (SE)
WBC count (×10 9 /l)
Survival differences between the DS-ALL and non-DS-ALL patients in the NOPHO ALL-1992/2000 protocols
Among the 4,637 non-DS-ALL patients, 1,554 and 1,128 were treated according to ALL-1992 and ALL-2000, respectively. There were no significant differences (P = 0.76) in 5-year EFS of the non-DS-ALL patients between these two protocols [pEFS 0.782 (0.010) vs. pEFS 0.785 (0.013)] or in 5-year OS (P = 0.51) [pOS 0.889 (0.008) vs. pOS 0.902 (0.009)]. These two protocols could hence be combined when comparing DS-ALL and non-DS-ALL.
Significant difference ( P = 0.005) a in types of event in DS-ALL and non-DS-ALL patients treated according to the ALL-1992/2000 protocols
DS-ALL (% of events)
non-DS-ALL (% of events)
Comparison of survival data between the DS-ALL and non-DS-ALL patients treated according to the ALL-1992/2000 protocols
5-year EFS (SE)
5-year OS (SE)
The basic clinical features of this population-based DS-ALL patient cohort from the Nordic countries (Table 1) agree very well with previous studies on DS-ALL. For example, there were no infants, no case had mature B-cell leukemia or T-cell ALL, there were no differences between the DS-ALL and the non-DS-ALL patients in sex ratio, WBC counts, and median age at diagnosis, and the platelet counts were lower in DS-ALL [4, 8, 11, 13, 20, 26]. Thus, the present series is clearly representative of DS-ALL.
Since our study includes ALL cases diagnosed during the last three decades, it provides data pertinent to possible time-related aspects of diagnostic routines. We had anticipated that there could have been a bias as regards reporting DS-ALL cases to the registry during its early years, perhaps because some centres may have been less inclined to give intensive treatment to children with DS and/or to include them in the database. However, this was clearly not the case. The incidence of DS-ALL has been quite constant throughout the years, with no significant variation (Figure 1). Furthermore, we have no evidence that DS-ALL cases have been less well cytogenetically characterized through the years. In contrast, although karyotypic failures/missing data for DS-ALL were much more common 1981–1995 (21%) than between 1996 and 2010 (4.7%), the corresponding frequencies in non-DS-ALL were even higher (53% and 10%, respectively). This notwithstanding, whereas the proportion of abnormal karyotypes in DS-ALL has not increased greatly during the last few years, there has been a significant increase in the frequency of abnormal karyotypes in non-DS-ALL. We do not believe that this is due to technical advances in cytogenetic analyses only, which seem to have benefited non-DS-ALL more than DS-ALL, but rather to true biological differences.
As alluded to above, DS-ALL significantly less often displayed an abnormal karyotype (60%) compared with non-DS-ALL (79%) (Table 2). A similarly low frequency of clonal abnormalities (54%) was also noted in a previous collaborative, international cytogenetic study on acute leukemia in DS patients . One reason for the higher proportion of normal karyotypes in DS-ALL could be that they more often harbor cytogenetically cryptic changes. In fact, deregulation of the CRLF2 gene, either through deletions of Xp22.33/Yp11.32 or by translocations between these regions and the immunoglobulin heavy chain locus (IGH@) at 14q32.33, both undetectable by conventional chromosome banding analysis, is substantially more common in DS-ALL than in non-DS-ALL [17, 18]. Furthermore, a “normal” karyotype in DS-ALL has an additional chromosome 21. Perhaps fewer additional, acquired aberrations are needed to establish the leukemia because of the presence of the “preleukaemic” trisomy 21, akin to what has been suggested for ML-DS, in which GATA1 mutations are only efficient in the context of +21c . In fact, it has been demonstrated that trisomy 21 as such perturbs fetal lymphopoiesis .
In contrast to the study by Forestier et al., in which it was reported that 11% of DS-ALL cases are high hyperdiploid, only 2% of the Nordic cases had a modal number between 51 and 67 chromosomes  (Table 2). The reason(s) for this pronounced difference is unclear. Although high hyperdiploid cells are notoriously difficult to culture in vitro and many cases may escape detection, masquerading as karyotypically normal or cytogenetic failures [33, 34], we deem it unlikely that the low frequency of such cases in our cohort is due to technical issues. First, the proportion of high hyperdiploid cases among the non-DS-ALL patients (33%) is on a par with other study groups . Second, DNA index analyses of pediatric leukemias are performed in clinical routine, according to the NOPHO protocols, and they should have identified high hyperdiploid cases that were cytogenetically missed. Third, most laboratories in the Nordic countries use interphase FISH analyses to identify t(12;21) and all use FISH to screen for iAMP21. Since the vast majority of high hyperdiploid ALL have tetrasomy 21 , such analyses would also identify extra copies of chromosome 21 and hence suggest the presence of high hyperdiploidy. Furthermore, Heerema et al.  reported only four DS patients (0.8%) among 480 high hyperdiploid ALL cases. Thus, we believe that there may have been a bias in reporting high hyperdiploid DS-ALL cases in Forestier et al..
Other ALL-associated abnormalities that were less common, but not significantly so, in DS-ALL were t(1;19), t(9;22), 11q23/MLL rearrangements, and t(12;21) (Table 2). However, the frequencies of t(1;19), t(9;22), and t(12;21) were slightly higher in this study compared with those reported in Forestier et al.. This most likely reflects that targeted analyses for these abnormalities were performed in a larger proportion of Nordic DS-ALL cases. As regards iAMP21, this aberration has been actively screened for only in the ongoing NOPHO ALL-2008 protocol. So far, none of 12 DS-ALL cases treated according to this protocol has harbored an iAMP21 whereas it has been detected in 1% of non-DS-ALL cases (Table 2). The low frequency of iAMP21 in DS-ALL agrees well with data from UK MRC ALL97 . The only structural abnormality that seems to be more common in DS-ALL is t(8;14)(q11;q32) (Table 2), something that has been noted also in several previous studies [38–40]. Why most ALL-associated abnormalities are less frequent or, as regards t(8;14), more common in DS-ALL remain enigmatic.
The distribution of types of event differed significantly between the DS-ALL and non-DS-ALL patients (Table 4). The fact that induction failure was more common in the former group was not surprising; this has been repeatedly observed [4, 11, 41]. However, the lack of secondary malignant neoplasms (SMN) after treatment for DS-ALL is noteworthy – none of the 128 DS-ALL patients in the Nordic countries has developed SMN. Although not stressed in previous studies, this seems to be the case also in several other treatment trials, such as those from the Berlin-Frankfurt-Münster (BFM), Children's Cancer Group (CCG), and Italian Association of Pediatric Hematology and Oncology (AIEOP) groups [10, 11, 41, 42]. It is well known that individuals with DS in general have a lower incidence of solid tumors than non-DS-individuals , with possible explanations including gains of tumor suppressor genes on chromosome 21, impaired angiogenesis, and accelerated cell ageing . Whether such mechanisms could be involved in preventing SMN in DS patients is unclear, not least considering that most SMN are not solid tumors but rather myelodysplastic syndromes and acute myeloid leukemia, with the latter being clearly overrepresented in DS as a de novo disease .
The only factor associated with survival in DS-ALL was the WBC count. Patients with a WBC count ≥50 × 109/l had significantly inferior 5-year EFS and 5-year OS compared with those with lower counts (Table 3; Figure 3). Although not surprising – a high WBC count is a well-established risk factor in pediatric non-DS-ALL  – this has not been clearly demonstrated in DS-ALL previously.
Data on improved outcome for DS-ALL have emerged during the last few years from some treatment trials [41, 42, 44], suggesting that induction and/or maintenance therapy has improved for this patient cohort. However, in the present study, both EFS and OS were still inferior for the DS-ALL cohort in the combined ALL-1992/2000 protocols (Figure 4; Table 5). Whether this depends on insufficient treatment intensity , clinical decision making for relapsed DS-ALL, or on treatment- and/or syndrome-related comorbidity  remains to be evaluated in detail.
This work was supported by the Swedish Childhood Cancer Foundation, the Swedish Cancer Society, and the Swedish Research Council.
- Krivit W, Good RA: Simultaneous occurrence of mongolism and leukemia: report of a nationwide survey. AMA J Dis Child. 1957, 94: 289-293.View ArticlePubMedGoogle Scholar
- Hasle H, Clemmensen IH, Mikkelsen M: Risks of leukaemia and solid tumours in individuals with Down's syndrome. Lancet. 2000, 355: 165-169.View ArticlePubMedGoogle Scholar
- Lange B: The management of neoplastic disorders of haematopoiesis in children with Down's syndrome. Br J Haematol. 2000, 10: 512-524.View ArticleGoogle Scholar
- Zeller B, Gustafsson G, Forestier E, Abrahamsson J, Clausen N, Heldrup J, Hovi L, Jonmundsson G, Lie SO, Glomstein A, Hasle H: Acute leukaemia in children with Down syndrome: a population-based Nordic study. Br J Haematol. 2005, 128: 797-804.View ArticlePubMedGoogle Scholar
- Maloney KW, Carroll WL, Carroll AJ, Devidas M, Borowitz MJ, Martin PL, Pullen J, Whitlock JA, Willman CL, Winick NJ, Camitta BM, Hunger SP: Down syndrome childhood acute lymphoblastic leukemia has a unique spectrum of sentinel cytogenetic lesions that influences treatment outcome: a report from the Children's Oncology Group. Blood. 2010, 116: 1045-1050.PubMed CentralView ArticlePubMedGoogle Scholar
- Seewald L, Taub JW, Maloney KW, McCabe ER: Acute leukemias in children with Down syndrome. Mol Genet Metab. 2012, 107: 25-30.View ArticlePubMedGoogle Scholar
- Day SM, Strauss DJ, Shavelle RM, Reynolds RJ: Mortality and causes of death in persons with Down syndrome in California. Dev Med Child Neurol. 2005, 47: 171-176.View ArticlePubMedGoogle Scholar
- Robison LL, Nesbit ME, Sather HN, Level C, Shahidi N, Kennedy A, Hammond D: Down syndrome and acute leukemia in children: a 10-year retrospective survey from Childrens Cancer Study Group. J Pediatr. 1984, 105: 235-242.View ArticlePubMedGoogle Scholar
- Levitt GA, Stiller CA, Chessells JM: Prognosis of Down's syndrome with acute leukaemia. Arch Dis Child. 1990, 65: 212-216.PubMed CentralView ArticlePubMedGoogle Scholar
- Dördelmann M, Schrappe M, Reiter A, Zimmermann M, Graf N, Schott G, Lampert F, Harbott J, Niemeyer C, Ritter J, Dörffel W, Nessler G, Kühl J, Riehm H: Down's syndrome in childhood acute lymphoblastic leukemia: clinical characteristics and treatment outcome in four consecutive BFM trials. Leukemia. 1998, 12: 645-651.View ArticlePubMedGoogle Scholar
- Whitlock JA, Sather HN, Gaynon P, Robison LL, Wells RJ, Trigg M, Heerema NA, Bhatia S: Clinical characteristics and outcome of children with Down syndrome and acute lymphoblastic leukemia: a Children's Cancer Group study. Blood. 2005, 106: 4043-4049.View ArticlePubMedGoogle Scholar
- Henry E, Walker D, Wiedmeier SE, Christensen RD: Hematological abnormalities during the first week of life among neonates with Down syndrome: data from a multihospital healthcare system. Am J Med Genet A. 2007, 143: 42-50.View ArticleGoogle Scholar
- Maloney KW: Acute lymphoblastic leukaemia in children with Down syndrome: an updated review. Br J Haematol. 2011, 155: 420-425.View ArticlePubMedGoogle Scholar
- Roy A, Roberts I, Vyas P: Biology and management of transient abnormal myelopoiesis (TAM) in children with Down syndrome. Semin Fetal Neonatal Med. 2012, 17: 196-201.View ArticlePubMedGoogle Scholar
- Bercovich D, Ganmore I, Scott LM, Wainreb G, Birger Y, Elimelech A, Shochat C, Cazzaniga G, Biondi A, Basso G, Cario G, Schrappe M, Stanulla M, Strehl S, Haas OA, Mann G, Binder V, Borkhardt A, Kempski H, Trka J, Bielorei B, Avigad S, Stark B, Smith O, Dastugue N, Bourquin JP, Tal NB, Green AR, Izraeli S: Mutations of JAK2 in acute lymphoblastic leukaemias associated with Down's syndrome. Lancet. 2008, 372: 1484-1492.View ArticlePubMedGoogle Scholar
- Kearney L, Gonzalez De Castro D, Yeung J, Procter J, Horsley SW, Eguchi-Ishimae M, Bateman CM, Anderson K, Chaplin T, Young BD, Harrison CJ, Kempski H, So CWE, Ford AM, Greaves M: Specific JAK2 mutation (JAK2R683) and multiple gene deletions in Down syndrome acute lymphoblastic leukaemia. Blood. 2009, 113: 646-648.View ArticlePubMedGoogle Scholar
- Mullighan CG, Collins-Underwood JR, Phillips LAA, Loudin MG, Liu W, Zhang J, Ma J, Coustan-Smith E, Harvey RC, Willman CL, Mikhail FM, Meyer J, Carroll AJ, Williams RT, Cheng J, Heerema NA, Basso G, Pession A, Pui C-H, Raimondi SC, Hunger SP, Downing JR, Carroll WL, Rabin KR: Rearrangement of CRLF2 in B-progenitor and Down syndrome-associated acute lymphoblastic leukemia. Nat Genet. 2009, 41: 1243-1246.PubMed CentralView ArticlePubMedGoogle Scholar
- Russell LJ, Capasso M, Vater I, Akasaka T, Bernard OA, Calasanz MJ, Chandrasekaran T, Chapiro E, Gesk S, Griffiths M, Guttery DS, Haferlach C, Harder L, Heidenreich O, Irving J, Kearney L, Nguyen-Khac F, Machado L, Minto L, Majid A, Moorman AV, Morrison H, Rand V, Strefford JC, Schwab C, Tönnies H, Dyer MJS, Siebert R, Harrison CJ: Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. Blood. 2009, 114: 2688-2698.View ArticlePubMedGoogle Scholar
- Ragab AH, Abdel-Mageed A, Shuster JJ, Frankel LS, Pullen J, van Eys J, Sullivan MP, Boyett J, Borowitz M, Crist WM: Clinical characteristics and treatment outcome of children with acute lymphocytic leukemia and Down's syndrome. A Pediatric Oncology Group study. Cancer. 1991, 67: 1057-1063.View ArticlePubMedGoogle Scholar
- Pui C-H, Raimondi SC, Borowitz MJ, Land VJ, Behm FG, Pullen DJ, Hancock ML, Shuster JJ, Steuber CP, Crist WM, Civin CI, Carroll AJ: Immunophenotypes and karyotypes of leukemic cells in children with Down syndrome and acute lymphoblastic leukemia. J Clin Oncol. 1993, 11: 1361-1367.PubMedGoogle Scholar
- Chessells JM, Harrison G, Richards SM, Bailey CC, Hill FG, Gibson BE, Hann IM: Down's syndrome and acute lymphoblastic leukaemia: clinical features and response to treatment. Arch Dis Child. 2001, 85: 321-325.PubMed CentralView ArticlePubMedGoogle Scholar
- Kawamata N, Ogawa S, Zimmermann M, Kato M, Sanada M, Hemminki K, Yamatomo G, Nannya Y, Koehler R, Flohr T, Miller CW, Harbott J, Ludwig W-D, Stanulla M, Schrappe M, Bartram CR, Koeffler HP: Molecular allelokaryotyping of pediatric acute lymphoblastic leukemias by high-resolution single nucleotide polymorphism oligonucleotide genomic microarray. Blood. 2008, 111: 776-784.PubMed CentralView ArticlePubMedGoogle Scholar
- Hertzberg L, Vendramini E, Ganmore I, Cazzaniga G, Schmitz M, Chalker J, Shiloh R, Iacobucci I, Shochat C, Zeligson S, Cario G, Stanulla M, Strehl S, Russell LJ, Harrison CJ, Bornhauser B, Yoda A, Rechavi G, Bercovich D, Borkhardt A, Kempski H, te Kronnie G, Bourquin J-P, Domany E, Izraeli S: Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group. Blood. 2010, 115: 1006-1017.View ArticlePubMedGoogle Scholar
- Loudin MG, Wang J, Eastwood Leung HC, Gurusiddappa S, Meyer J, Condos G, Morrison D, Tsimelzon A, Devidas M, Heerema NA, Carroll AJ, Plon SE, Hunger SP, Basso G, Pession A, Bhojwani D, Carroll WL, Rabin KR: Genomic profiling in Down syndrome acute lymphoblastic leukemia identifies histone gene deletions associated with altered methylation profiles. Leukemia. 2011, 25: 1555-1563.PubMed CentralView ArticlePubMedGoogle Scholar
- Lundin C, Hjorth L, Behrendtz M, Nordgren A, Palmqvist L, Andersen MK, Biloglav A, Forestier E, Paulsson K, Johansson B: High frequency of BTG1 deletions in acute lymphoblastic leukemia in children with Down syndrome. Genes Chromosomes Cancer. 2012, 51: 196-206.View ArticlePubMedGoogle Scholar
- Forestier E, Izraeli S, Beverloo B, Haas O, Pession A, Michalová K, Stark B, Harrison CJ, Teigler-Schlegel A, Johansson B: Cytogenetic features of acute lymphoblastic and myeloid leukemias in pediatric patients with Down syndrome: an iBFM-SG study. Blood. 2008, 111: 1575-1583.View ArticlePubMedGoogle Scholar
- Paulsson K, Forestier E, Andersen MK, Autio K, Barbany G, Borgström G, Cavelier L, Golovleva I, Heim S, Heinonen K, Hovland R, Johannsson JH, Kjeldsen E, Nordgren A, Palmqvist L, Johansson B: High modal number and triple trisomies are highly correlated favorable factors in childhood B-cell precursor high hyperdiploid acute lymphoblastic leukemia treated according to the NOPHO ALL 1992/2000 protocols. Haematologica. 2013, 98: 1424-1432.PubMed CentralView ArticlePubMedGoogle Scholar
- Buitenkamp TD, Izraeli S, Zimmermann M, Forestier E, Heerema NA, van den Heuvel-Eibrink MM, Pieters R, Korbijn CM, Silverman LB, Schmiegelow K, Liang DC, Horibe K, Arico M, Biondi A, Basso G, Rabin KR, Schrappe M, Cario G, Mann G, Morak M, Panzer-Grümayer R, Mondelaers V, Lammens T, Cavé H, Stark B, Ganmore I, Moorman AV, Vora A, Hunger SP, Pui CH: Acute lymphoblastic leukemia in children with Down syndrome: a retrospective analysis from the Ponte di Legno study group. Blood. 2014, 123: 70-77.PubMed CentralView ArticlePubMedGoogle Scholar
- Gustafsson G, Schmiegelow K, Forestier E, Clausen N, Glomstein A, Jonmundsson G, Mellander L, Makipernaa A, Nygaard R, Saarinen-Pihkala UM: Improving outcome through two decades in childhood ALL in the Nordic countries: the impact of high-dose methotrexate in the reduction of CNS irradiation. Leukemia. 2000, 14: 2267-2275.View ArticlePubMedGoogle Scholar
- Schmiegelow K, Forestier E, Hellebostad M, Heyman M, Kristinsson J, Söderhäll S, Taskinen M: Long-term results of NOPHO ALL-92 and ALL-2000 studies of childhood acute lymphoblastic leukemia. Leukemia. 2010, 24: 345-354.View ArticlePubMedGoogle Scholar
- Malinge S, Izraeli S, Crispino JD: Insights into the manifestations, outcomes, and mechanisms of leukemogenesis in Down syndrome. Blood. 2009, 113: 2619-2628.PubMed CentralView ArticlePubMedGoogle Scholar
- Roy A, Cowan G, Mead AJ, Filippi S, Bohn G, Chaidos A, Tunstall O, Chan JK, Choolani M, Bennett P, Kumar S, Atkinson D, Wyatt-Ashmead J, Hu M, Stumpf MP, Goudevenou K, O'Connor D, Chou ST, Weiss MJ, Karadimitris A, Jacobsen SE, Vyas P, Roberts I: Perturbation of fetal liver hematopoietic stem and progenitor cell development by trisomy 21. Proc Natl Acad Sci U S A. 2012, 109: 17579-17584.PubMed CentralView ArticlePubMedGoogle Scholar
- Harrison CJ, Moorman AV, Barber KE, Broadfield ZJ, Cheung KL, Harris RL, Jalali GR, Robinson HM, Strefford JC, Stewart A, Wright S, Griffiths M, Ross FM, Harewood H, Martineau M: Interphase molecular cytogenetic screening for chromosomal abnormalities of prognostic significance in childhood acute lymphoblastic leukaemia: a UK Cancer Cytogenetics group study. Br J Haematol. 2005, 129: 520-530.View ArticlePubMedGoogle Scholar
- Paulsson K, Johansson B: High hyperdiploid childhood acute lymphoblastic leukemia. Genes Chromosomes Cancer. 2009, 48: 637-660.View ArticlePubMedGoogle Scholar
- Paulsson K, Forestier E, Lilljebjörn H, Heldrup J, Behrendtz M, Young BD, Johansson B: Genetic landscape of high hyperdiploid childhood acute lymphoblastic leukemia. Proc Natl Acad Sci U S A. 2010, 107: 21719-21724.PubMed CentralView ArticlePubMedGoogle Scholar
- Heerema NA, Sather HN, Sensel MG, Zhang T, Hutchinson RJ, Nachman JB, Lange BJ, Steinherz PG, Bostrom BC, Reaman GH, Gaynon PS, Uckun FM: Prognostic impact of trisomies of chromosomes 10, 17, and 5 among children with acute lymphoblastic leukemia and high hyperdiploidy (>50 chromosomes). J Clin Oncol. 2000, 18: 1876-1887.PubMedGoogle Scholar
- Moorman AV, Richards SM, Robinson HM, Strefford JC, Gibson BES, Kinsey SE, Eden TOB, Vora AJ, Mitchell CD, Harrison CJ: Prognosis of children with acute lymphoblastic leukemia (ALL) and intrachromosomal amplification of chromosome 21 (iAMP21). Blood. 2007, 109: 2327-2330.View ArticlePubMedGoogle Scholar
- Secker-Walker LM, Hawkins JM, Prentice HG, Mackie PH, Heerema NA, Provisor AJ: Two Down syndrome patients with an acquired translocation, t(8;14)(q11;q32), in early B-lineage acute lymphoblastic leukemia. Cancer Genet Cytogenet. 1993, 70: 148-150.View ArticlePubMedGoogle Scholar
- Lundin C, Heldrup J, Ahlgren T, Olofsson T, Johansson B: B-cell precursor t(8;14)(q11;q32)-positive acute lymphoblastic leukemia in children is strongly associated with Down syndrome or with a concomitant Philadelphia chromosome. Eur J Haematol. 2009, 82: 46-53.View ArticlePubMedGoogle Scholar
- Messinger YH, Higgins RR, Devidas M, Hunger SP, Carroll AJ, Heerema NA: Pediatric acute lymphoblastic leukemia with a t(8;14)(q11.2;q32): B-cell disease with a high proportion of Down syndrome: a Children's Oncology Group study. Cancer Genet. 2012, 205: 453-458.PubMed CentralView ArticlePubMedGoogle Scholar
- Arico M, Ziino O, Valsecchi MG, Cazzaniga G, Baronci C, Messina C, Pession A, Santoro N, Basso G, Conter V: Acute lymphoblastic leukemia and Down syndrome: presenting features and treatment outcome in the experience of the Italian Association of Pediatric Hematology and Oncology (AIEOP). Cancer. 2008, 113: 515-521.View ArticlePubMedGoogle Scholar
- Meyr F, Escherich G, Mann G, Klingebiel T, Kulozik A, Rossig C, Schrappe M, Henze G, von Stackelberg A, Hitzler J: Outcomes of treatment for relapsed acute lymphoblastic leukaemia in children with Down syndrome. Br J Haematol. 2013, 162: 98-106.View ArticlePubMedGoogle Scholar
- Nižetić D, Groet J: Tumorigenesis in Down’s syndrome: big lessons from a small chromosome. Nat Rev Cancer. 2012, 12: 721-732.View ArticlePubMedGoogle Scholar
- Shah N, Al-Ahmari A, Al-Yamani A, Dupuis L, Stephens D, Hitzler J: Outcome and toxicity of chemotherapy for acute lymphoblastic leukemia in children with Down syndrome. Pediatr Blood Cancer. 2009, 52: 14-19.View ArticlePubMedGoogle Scholar
- Bohnstedt C, Levinsen M, Rosthøj S, Zeller B, Taskinen M, Hafsteinsdottir S, Björgvinsdóttir H, Heyman M, Schmiegelow K: Physicians compliance during maintenance therapy in children with Down syndrome and acute lymphoblastic leukemia. Leukemia. 2013, 27: 866-870.View ArticlePubMedGoogle Scholar
- Patrick K, Wade R, Goulden N, Rowntree C, Hough R, Moorman AV, Mitchell CD, Vora A: Outcome of Down syndrome associated acute lymphoblastic leukaemia treated on a contemporary protocol. Br J Haematol. in pressGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.