Skip to main content

Challenges and advances in clinical applications of mesenchymal stromal cells

Abstract

Mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells, have been intensely investigated for clinical applications within the last decades. However, the majority of registered clinical trials applying MSC therapy for diverse human diseases have fallen short of expectations, despite the encouraging pre-clinical outcomes in varied animal disease models. This can be attributable to inconsistent criteria for MSCs identity across studies and their inherited heterogeneity. Nowadays, with the emergence of advanced biological techniques and substantial improvements in bio-engineered materials, strategies have been developed to overcome clinical challenges in MSC application. Here in this review, we will discuss the major challenges of MSC therapies in clinical application, the factors impacting the diversity of MSCs, the potential approaches that modify MSC products with the highest therapeutic potential, and finally the usage of MSCs for COVID-19 pandemic disease.

Background

Mesenchymal stromal cells (MSCs) are pluripotent non-hematopoietic stem cells with self-renewal capability [1] and being intensively investigated in clinical trials. Since the discovery of MSCs from bone marrow by Friedenstein in 1970s, MSCs have been isolated from various sources including muscle, umbilical cord, liver, placenta, skin, amniotic fluid, synovial membrane, and tooth root [2, 3], and tested in amounts of preclinical and clinical studies (Fig. 1). It is now understood that MSCs have wide-ranging physiological effects including the maintenance of tissue homeostasis and regeneration [4, 5], as well as the immunomodulatory activities suitable for therapeutic application [6]. So their indications have been expanded to graft-versus-host disease (GVHD), multiple sclerosis (MS), Crohn’s disease (CD), amyotrophic lateral sclerosis (ALS), myocardial infarction (MI), and acute respiratory distress syndrome (ARDS) [7,8,9].

Fig.1
figure1

Various sources of MSCs used in the registered clinical trials. MSCs isolated from bone marrow are most widely applied in clinical trials, followed by those from umbilical cord and adipose. MSCs from muscles, tooth are also used

Over 300 clinical trials of MSC therapies have been completed in patients including but not limited to degenerative or autoimmune diseases (Table 1 lists some of the representative completed studies). Overall, MSCs have exhibited tolerable safety profile and demonstrated promising therapeutic benefits in some clinical settings, which led to regulatory approvals of MSCs in a few countries. In 2011, the Ministry of Food and Drug Safety (Korea FDA) approved Cartistem®, a MSC product derived from umbilical cord blood and developed by Medipost for the treatment of traumatic or degenerative osteoarthritis [10]. Thereafter, more MSC products including HeartiCellgram®, Mesoblast, TiGenix, and Stempeutics, were approved by regulatory authorities worldwide for the treatment of a variety of diseases. In the USA, Ryoncil (remestemcel-L) is promising to be the first FDA-approved GVHD treatment for children younger than 12, but is still in the stage of safety verification. The amount of clinics offering exogenous stem cell therapies has doubled from 2009 to 2014 in the USA. This boom in stem cell clinics with 351 companies putting stem cells for sale in 570 clinics in 2016 indicated the mal-practice of the MSC therapies [11]. Considering the fact that many of the applied exogenous stem cell therapies lack confirmation on safety and effectiveness from large-scale clinical trials and are even illegal, these medical mal-practices do threaten the development of MSC therapies [12].

Table 1 Some representative registered clinical trials of MSC therapies

In this review, we will focus on the major challenges of MSC therapies and the underlying factors leading to the failure of clinical trials. Recent advances and prospects concerning the translation of MSC techniques into clinical practices will also be discussed.

Challenges in technology transfer of MSCs from bench to bedside

Although transferring MSCs from bench to bedside is theoretically achievable, substantial failures have been reported in many early- or late-stage clinical trials, which account for the disapproval of many products by FDA [13]. Factors contributing to the failure of MSC clinical development include but not limited to the poor-quality control and inconsistent characteristics of MSCs in terms of immunocompatibility, stability, heterogeneity, differentiation, and migratory capacity [14, 15] (Fig. 2).

Fig. 2
figure2

The main challenges in clinical applications of MSCs. During preparation of the MSC products, the main challenges include: (1) heterogeneity of MSCs resulted from donor variations such as the health status, genetics, gender, and age. (2) The varying degree of stability of stemness and differentiation capacities between MSCs isolated from different sources, such as bone marrow, adipose tissue, umbilical cord, or muscles. (3) The varying level of expansion capacities under different culture conditions, including confluence, culture surface, oxygen levels, flasks/bioreactors, passage number, and cell surface modifications. At the state of application, challenges remain due to the influence of (1) the homing or migratory capacity of MSCs under different administration route (local/systemic), injection site, infusion time, and cell carrier materials. (2) The immune compatibility between donors and recipients is the key to reduce the risk of rejection, but is affected by environmental inflammatory molecules which could induce distinct expression of MHC-II in MSCs. (3) The complex effective components released by MSCs depending on the host microenvironment (inflammation status, hypoxia, and ECM), which can result in highly variable factors shaping distinct functions of MSCs

Immunocompatibility of MSCs

MSCs were immune privileged due to the low expression of MHC-I and HLA-I, and no expression of HLA-II or costimulatory factors such as CD40, CD80 and CD86. MSCs can be transplanted as allogeneic cells with a low risk of rejection. Generally, the original MSCs are believed to have low immunogenicity [16]. Most MSC products are manufactured by amplifying a small number of cells obtained from donors, which can increase MSC immunogenicity caused by inappropriate processes and culture conditions. After MSCs infusion, the in vivo inflammatory molecules in turn increase MSC immunogenicity and further decrease MSCs viability and differentiation capacity, particularly when administrating xenogenic MSCs including human MSCs in animal models [17]. Although the primary immunogenicity of MSCs derived from in vitro experiments might be minimal, the secondary immunogenicity induced by in vivo positive feedback loops can cause the absence of efficacy reported in most clinical trials.

Studies have shown that inflammatory molecules (such as interferon-γ), increased cell density, and/or serum deprivation can induce high expression of MHC-II in MSCs, while TGF-β suppresses MHC-II expression [18]. The immune compatibility between donors and recipients is the key to reduce the risk of rejection in the event of long-term treatments with repeated infusions, in conditions requiring promotion of transplanted bone marrow integration, or post-renal transplantation rejection treatments [19]. It has been reported that repeated intra-articular injection of allogeneic MSCs is more likely to cause an adverse reaction than autologous cells when administered in the same manner [20]. The same observations were reported in horses treated with intracellular xenogen-contaminated autologous MSCs (such as FBS) or non-xenogen-contaminated allogeneic MSCs [21].

MSCs of high quality is the first step to ensure the safety and efficacy in clinical trials. Understanding the molecular and cellular mechanisms underlying the immune incompatibility of MSCs will help to improve the manufacture of MSC products.

Stemness stability and differentiation of MSCs

MSCs have mesodermal lineage differentiation potential and the potential to regulate tissue regeneration by mediating tissue and organ repair, as well as replacing damaged cells [22]. Different tissue-derived MSCs exhibit tendencies to differentiate into different end-stage lineage cells [23, 24], and such regeneration and differentiation contribute to distinctive clinical efficacy.

Several laboratories have analyzed the proteome modifications associated with MSCs differentiation [25, 26]. They indicated that ‘‘stemness’’ genes were highly expressed in undifferentiated and de-differentiated MSCs [27, 28]. These highly stemness-related gene clusters in MSCs have been found to be mainly involved in the proliferation, differentiation, and migration [29]. When MSCs differentiated into osteoblasts, chondrocytes, and adipocytes, expressions of these genes significantly decreased, underlining their unique characteristics. Table 2 lists typical stemness genes of MSCs.

Table 2 Some typical stemness genes of MSCs

Serial passaging in long-term culture could negatively affect the expression of stemness genes [48, 49]. A previous study indicated that CD13, CD29, CD44, CD73, CD90, CD105, and CD106 in MSCs are down-regulated during culture expansion compared to MSCs in the stromal fraction [50]. The senescence-related proteins p53, p21, and p16 expressed under different conditions [51]. Rene et al. reported that after short-term in vitro culture, wild-type MSCs became senescent, and p21(−/−)p53(+/+) MSCs showed an elevated spontaneous apoptosis rate but no sign of tumoral transformation [52]. On the other hand, Mclean et al. discovered cancer-associated MSCs (CA-MSCs), which are determined by the expression of CD44, CD73, and CD90, exhibited the upregulation of the TGF-β superfamily/bone morphogenetic protein (BMP) family [53], and MSCs harbored the potential to differentiate into cancer-associated fibroblasts (CAFs) at latter passages [54,55,56,57]. The malignant phenotypes of MSCs associated with CAFs could express Meflin, which is also a marker of MSCs maintaining their undifferentiated state [57,58,59].

To provide sufficient MSCs for clinical trials, MSCs need to be amplified in a large scale, which will inevitably face the issue of MSCs senescence and subsequent modifications of gene expressions [60]. Therefore, the long-term culture of MSCs often results in decreased proliferation and differentiation capacities and shortened life expectancy [61]. A standardized manufacturing process is essential for the success of clinical trials. Though the above molecules have been found to mediate the stemness of MSCs and regulate their differentiation, it remains challenging to control the fate of MSCs in a complex in vivo environment.

Heterogeneity of MSCs

Heterogeneity of MSCs is determined by multiple factors including but not limited to donors and tissue sources, cell populations, culture conditions, cell isolation techniques, cryoprotective and thawing protocols [62,63,64] (Fig. 3).

Fig. 3
figure3

MSCs exhibit heterogeneity at multiple levels. Heterogeneity of MSCs is determined by factors at multiple levels. (1) Donors at different health status, genetics, gender, and age may result in variations. (2) Tissue from different sources exhibits distinct characteristics, therefore leading to heterogeneity. (3) Cell isolation techniques may lead to distinct purity and sub-populations. (4) Cell culture environment and preservation conditions could affect the expansion and states of MSCs, therefore also affecting the heterogeneity

MSCs were defined as adherent cells with a spindle-shaped morphology in standard culture conditions according to the minimal criteria developed by the International Society of Cell Therapy in 2006 [65]. They were characterized by the following features: (1) expression of CD105, CD73, and CD90, but no expression of CD45, CD34, CD14 or CD11b, CD79a, CD19, or HLA-DR; (2) capacity to differentiate into osteoblasts, adipocytes, and chondroblasts in vitro. However, these criteria were insufficient to define MSCs as variations exist at multiple levels. First, MSCs from different donors have distinct functions due to differences in age, health condition, and other individual characteristics. Second, MSCs from different tissues ranging from adipose tissue to bone marrow could be distinct in terms of surface markers and differentiation capacities. This variation probably results from different biological, chemical, and mechanical stresses in stem cell niches, though the culture conditions are similar in vitro. Moreover, MSCs form clones, and cell heterogeneity exists both inter-clonally and intra-clonally. Extracellular matrix genes and osteogenic transcription factor-related genes show increased expression in highly osteogenic clones compared to poor osteogenic clones. Cell morphology and differentiation ability within one clone can also be remarkably different. For instance, cells located at the outer periphery express higher levels of genes related to cell proliferation (MKI67 and PODXL), while extracellular matrix genes (VCAM1) tend to be expressed in interior MSCs [66].

To identify specific cell subsets in heterogeneous MSCs, researchers have been continuously exploring characteristic cell surface markers and molecular signatures. Single cell-derived colony with rapidly dividing cells shows high colony-forming efficiency. STRO-1, CD146, and CD271 have been identified as cell surface markers for this subset [67]. However, cell subsets sharing similar surface markers would exhibit different chondrogenic differentiation capacities even under the same culture conditions [68]. RNA sequencing and microarray analysis have showed transcriptional signals predicting differentiation potential. Osterix and distal-less homeobox5 are the main transcription factors involved in osteoblast differentiation, while peroxisome proliferator-activated receptor gamma (PPAR-γ) and CCAAT/enhancer-binding protein alpha are associated with adipogenic potential [69]. In addition, MSCs with specific surface markers of differentiation potential may present various physiological functions [70]. For example, CD105 + MSCs exhibited myogenic potential assisting the repairment of the infarcted myocardium [71], while CD106 + MSCs showed enhanced multipotency and immunosuppressive ability [72]. Increasing evidence shows that MSCs comprise multiple subsets with specific surface markers. More work is needed to define these subpopulations based on biomarkers and biological functions.

Directed migratory capacity of MSCs

The therapeutic efficacy of MSCs is highly dependent on their in vivo migration and homing capacities. The migrating direction is determined by chemokine receptors expressed on MSCs and chemokines in tissues [73]. Freshly isolated MSCs have a good homing effect, which is decreased after somatic expansion. For example, the chemokine receptor CXCR4 is highly expressed on primary bone marrow MSCs, but gradually lost with passages, resulting in the less recognition of its ligand CXCL12 (also known as SDF-1α) [74, 75]. Together, the primary MSCs are expected to have a better therapeutic efficacy due to more potent migration capacity.

However, the expression profile of chemokines in damaged tissues is often not compatible with that of receptors on MSCs. For instance, CXCL1, CXCL2 and CCL7 increased in infarcted myocardium, while expression of corresponding receptors (CCR1 and CXCR2) on MSCs was very low, resulting in low efficiency in the migration of MSCs to infarct sites [76]. To improve the migration rate, MSCs are genetically modified to express specific chemokine receptors [73]. For example, CCR7-modified MSCs efficiently migrated to secondary lymphoid organs and demonstrated significant clinical efficacy in the GVHD mouse model [77, 78]. CXCR5-modified MSCs migrated to the damaged sites by binding to CXCL13, which was highly expressed in damaged tissues [79]. Taken together, genetically modified MSCs are an independent treatment entity and could be used as targeted therapy.

The delivery of MSCs emerges as a prerequisite to the unfoldment of their full therapeutic potential. Different delivery routes could affect cell homing, survival, and paracrine function. Systemic delivery is considered a reasonable approach. However, the reported effect in terms of homing rate, survival rate, and maintenance of cellular function was modest and transient [80] for reasons including poor migration rate from vessels to tissues and high retention rate in the liver, lungs, and spleen [81]. In contrast to intravenous delivery, intra-tissue or intra-organ delivery showed higher delivery retention and efficiency, as evidenced by a large body of studies [82]. However, clustering of MSCs and occlusions in microvasculature has been reported in some disease models such as myocardial infarction [83]. Walczak et al. reported that only cells with a diameter between 20 and 50 μm could avoid intracerebral entrapment [84]. Therefore, to maximize therapeutic efficacy, both the migratory capacity of MSCs and appropriate delivery methods should be considered.

Limited expansion of MSCs

Theoretically, MSCs can be expanded in vitro in traditional culture plates and flasks to any amount that meets experimental purpose. However, with prolonged culture duration and increased passage numbers, MSCs reach the Hayflick limit, exhibiting a marked decrease in proliferation with a transformation in morphology from a thin spindle shape to a flattened square shape. The cell density seeded in the culture containers also plays a role in the senescence of MSCs. Neuhuber et al. found the optimal cell growth of rat MSCs at 200 cells per cm2 compared with 20 cells or 2000 cells per cm2 [85]. In other studies, a relatively low density (~ 1.5–200 cells per cm2) was suggested to support better proliferation [86]. Alterations in autocrine secretion and contact inhibition may contribute to the slow growth at high density.

Large-scale expansion in 2D plates over long term also impacts stem cell characteristics of MSCs. According to Zhao et al., hUC-MSCs at various passages have multiple mutation spectra on signatures and functions, and cells at high passage showed declined therapeutic effect in aGVHD mouse model [87]. It has been shown that chondrogenic differentiation of MSCs in 2D culture is less efficient than that of MSCs in 3D culture [88]. Therefore, 3D expansion of MSCs was developed to prevent phenotypic changes caused by monolayers, where a broad and flattened morphology upon passaging was well preserved.

Moreover, MSCs have shown the capacity to differentiate into numerous cell types such as neural cells, hepatocyte-like cells, and pancreatic islet-like cells [89, 90]. The transient differentiation of MSCs into neural precursor-like cells may experience de-differentiation during extended culture [91]. Therefore, in vitro induction is often insufficient to yield pure functionally competent cells.

Taken together, developing the technique that can produce a huge number of cells rapidly and cost-effectively with guaranteed cell quality is paramount for the clinical progress of MSCs.

Effective components of MSC treatments

The secretion of cytoprotective factors by MSCs was first reported by Gnecchi and colleagues. They observed that Akt-MSCs (MSCs overexpressing Akt) prevented ventricular remodeling and improved the heart function following surgical myocardial infarction (MI). Since cell transplantation and myogenic pathways would be ineffective over such a brief interval, a new mechanism was proposed that the injected MSCs might act through releasing trophic factors that contribute to myocardial protection following an ischemic insult. This hypothesis was then confirmed by evident improvements in cardiac performance following injection of conditioned medium (CM) collected from hypoxic Akt-MSCs into an induced MI model, which protected ventricular cardiomyocytes with less apoptosis when subjected to a hypoxic condition [92].

In 2007, Dai et al. observed that MSCs-CM had a similar, albeit less intense, effect of MSCs in myocardial infarction, indicating that at least part of the effect observed following MSCs injection could be attributed to soluble factors [93]. In the context of neuronal damage, it has been established that the presence of BDNF, GDNF, NGF, and IGF in the MSCs secretome is necessary for the neuronal survival in vitro and in vivo [94, 95]. MSCs-CM has demonstrated therapeutic efficacy in some other disease models including chronic kidney disease, certain lung, and liver diseases [96, 97].

The paracrine effects of MSCs as an initial mechanism of action inspired further biological analysis of MSCs secretome [98]. Subsequent studies found more paracrine effectors, including soluble cytokines, growth factors, hormones, miRNAs, or lncRNAs that targeting a variety of cells such as immune cells and injured tissue cells [99]. In addition, the paracrine effectors could be loaded in extracellular vesicles (EVs) and exerted long-term effects [100]. In accordance, many studies have shown that MSC-derived EVs retain the biological activity of parental MSCs. It has been demonstrated that EVs showed a similar therapeutic effect as MSCs in selected animal models [101]. However, different studies found various effective components of MSCs in specific animal models and human diseases, and the interactions and functional differences between effectors remain elusive. Therefore, novel in-depth analytical techniques and platforms are warranted to investigate the MSCs secretome in the future.

Attempts to improve the therapeutic outcomes of MSCs

Although there were no attributable serious adverse events after MSC therapy, fever within 24 h and temporary pain at the injection sites are commonly occurred. Here we summarize four strategies to limit adverse events related to MSC treatments and improve the therapeutic outcomes, including genetic modifications or priming strategies to change the inherent characteristics of MSCs, and biomaterial strategies to modify the outside circumstances, and the usage of MSCs secretome (Fig. 4).

Fig. 4
figure4

Current attempts to improve MSC treatment. To improve the therapeutic efficiency of MSCs treatment, modification was made mainly in the following aspects: (1) genetic modification of MSCs by viral transduction or CRISPR/Cas9 techniques to engineer MSCs with enhanced homing, potency, or expansion capacities; (2) priming MSCs with small molecules, hypoxia, or structural stimulations by biomaterials to improve MSC function, survival, and therapeutic efficacy, thus boosting their therapeutic efficacy; (3) biomaterial strategies to improve the survival and function of MSCs by offering a scaffold for MSCs adherence, including modifications on dimensionality, stiffness, topographical cues, surface chemistry, and microstructure of biomaterials. (4) Utilize the MSCs secretome as a drug delivery platform for treatment

Biomaterial strategies to maintain more homogeneous MSCs

Biomaterials for delivering MSCs have been extensively investigated. These materials showed advantages in offering a scaffold for the adherence and survival of MSCs, as well as preserving the functional components MSCs secreted, thus elongating the effective durations in clinical treatment. However, the implantation of biomaterials could induce the foreign-body responses (FBR) in the host immune system, which can potentially result in fibrosis and failure of the implantation. Therefore, biomaterials suitable for MSCs were constructed to ameliorate the FBR and subsequent fibrotic encapsulation [102]. For example, loading MSCs with small-molecule encapsulating microparticles (MPs) can boost the duration of the products. MPs are composed of biocompatible materials that can be therapeutically tuned according to their composition, polymer molecular weight, drug loading, and release capacities [103]. MSCs loaded with degradable budesonide-containing MPs exhibited fourfold increase in IDO activity in vitro compared to MSCs without being pre-treated with budesonide [104]. This led to a twofold improvement in the suppression of peripheral blood mononuclear cells (PBMCs) activation following IFN-γ stimulation [105].

MSCs are typically delivered to a graft site using a decellularized extracellular matrix (ECM) scaffold. The advent of synthetic polymers has revolutionized tissue engineering. These polymers are highly tunable, homogenous, and cell-free materials and have a high batch-to-batch consistency taking the form of porous hydrogels, sponges, plates, or membranes [106, 107]. However, their unique properties could exert different influences on MSCs function. Table 3 summarizes the influence of biomaterials properties on the function of MSCs, including dimensionality, stiffness, topographical cues, surface chemistry, and microstructure of biomaterials.

Table 3 The properties of biomaterials affecting the function of MSCs

Genetic modification to produce MSCs with desired biologic function

Viral DNA transduction and mRNA/DNA transfection

To further optimize the therapeutic efficacy of MSCs, MSCs have been genetically engineered to produce trophic cytokines or other beneficial gene products in numerous preclinical models by transfecting MSCs with viral or non-viral vectors. Over the last few decades, these MSCs have successfully been engineered to express therapeutic peptides and proteins in animal models [119]. For instance, MSCs expressing thioredoxin-1 (Trx1, a powerful antioxidant, transcription factor and growth factor regulator) improved cardiac function in post-myocardial infarction rat models [120]. MSCs expressing IL-12 showed potent anticancer activity against melanoma, breast cancer, and hepatoma [121, 122]. And MSCs expressing interferon-γ inhibited tumor growth in mouse neuroblastoma and lung carcinoma models [123, 124]. In line with these advances achieved in animal models, several MSCs-based therapies are under clinical development (Table 4).

Table 4 Engineered MSCs for treatment reaching the clinical stage

However, both viral and non-viral vectors have some limitations. Non-viral vectors present transient gene expression and low-transfection efficiency, while viral transduction is associated with a higher risk of chromosomal instability, insertional mutagenesis, and proto-oncogene activation despite the inherent high transfection efficiency [125]. The adverse immune reactions induced by viral transduction were reported to impair the stability of transgenes [126, 127]. Therefore, the limitations and adverse responses should be valued when modifying MSCs by transfection.

Some studies made attempts on human-induced pluripotent stem cell (iPSC)-derived MSCs to obtain improved expandability. Actually, therapeutic transgenes could be inserted into iPSC-derived MSCs before MSCs derivation. This strategy could eliminate insertional mutation as well as guarantee stable expression of transgenes during prolonged expansion [128]. So iPSC-derived MSCs may be a candidate of MSCs for usage.

CRISPR-Cas9 technology to obtain highly homogeneous MSCs

With CRISPR/Cas9 technology, genetic modification of MSCs can be done with higher efficiency and specificity [129]. Compared to transcription activator like effector nuclease (TALEN) and the zinc-finger nucleases (ZFNs), CRISPR/Cas9 technology is faster, more economically efficient, and user-friendly [130]. CRISPR/Cas9-based gene manipulation has been widely employed in stem cell field particularly MSCs research, including gene knock-in, knock-out, activation or silence, etc.

CRISPR/Cas9-mediated gene knockdown in MSCs has been proved effective in treating diseases such as myocardial infarction [131]. Targeted gene knock-in promoted the differentiation capacity of MSCs and, in turn, ameliorated the insufficiency of functional cells in local sites [132]. Genetically modified MSCs have been evaluated in clinical trials. The TREAT-ME-1 study, an open-label, multicenter, and first-in-human Phase 1/2 trial, evaluated the safety, tolerability, and efficacy of genetically modified autologous MSC-apceth-101 treatment in patients with advanced gastrointestinal adenocarcinoma [133]. Further investigations are still needed to obtain unequivocal evidence on the differentiation and regeneration potentials of MSCs in vivo. Moreover, next-generation sequencing and genotypic techniques might serve as a new paradigm to improve the efficacy on targeting specific cell types for personalized medicine. CRISPR gene-engineered MSCs studies are illustrated in Table 5.

Table 5 The tests of modified MSCs using CRISPR-Cas9 technology

Despite the specificity of CRISPR/Cas technology in gene delivery [143], only one clinical trial of MSCs modified with CRISPR/Cas9 has been registered (NCT03855631).

“Priming” MSCs with small molecules to exogenously boost their therapeutic function

Given current manufacture of MSCs cannot meet the requirement for clinical trials in terms of production scale, the alternative is to boost the function of limited cells through priming MSCs. Priming has also been referred to as licensing or preconditioning, which is a concept commonly used in the field of immunology, and it has been adapted to the scope of stem cells [144, 145]. One of the commonly used strategies is priming MSCs with pro-inflammatory mediators, including IFN-γ, TNF-α, IL-1α, and IL-1β, and more priming approaches are being proposed to improve the function, survival, and therapeutic efficacy of MSCs [146, 147]. The priming approaches could be divided into three categories based on the stimulations: (a) MSCs priming with small molecules, (b) MSCs priming with hypoxia, (c) MSCs priming with biomaterials. Table 6 summarizes some representative priming MSCs.

Table 6 Representative priming MSCs in preclinical and clinical studies

“Priming” MSCs resulted in exogenously boosted therapeutic function in comparison with original state. Several “primed” MSC products have been applied clinically, with the most notable being NurOwn from Brainstorm Cell Therapeutics Company. NurOwn boosted the expression of multiple neurotrophic factors (NTFs) including GDNF, BDNF, VEGF, and HGF [173]. When administered to patients with neurodegenerative diseases, NurOwn delivered multiple NTFs as well as the immunomodulatory components secreted by MSCs. This combination demonstrated impressive therapeutic efficacy in a phase 2 clinical trial (NCT02017912), in which ALS patients got reduced ALS progression 24 months after NurOwn infusion compared to the controls [174]. So the indication of NurOwn has been expanded to include multiple sclerosis.

However, priming approaches of MSCs still have many limitations in clinical translation, such as induction of immunogenicity, high costs, variable effects, and lack of good manufacturing practices (GMP) suitable for clinical application [175]. Moreover, the long-term effect of priming MSCs has not been evaluated yet. Further studies are needed to evaluate (1) the effects of different priming approaches in clinic; (2) the best sources for MSCs isolation; (3) the epigenetic modifications, immunogenicity, and tumorigenicity of primed and non-primed MSCs; and (4) the appropriate GMP standards for quality control of MSC products, including quality of cryopreserved primed-MSCs at different passages.

Utilize the MSCs secretome as a drug delivery platform for treatment

The “secretome” of MSCs, including secretory proteins such as growth factors, cytokines, and chemokines and EVs such as microvesicles (MVs; 100–1000 nm diameter) and exosomes (40–150 nm diameter), has been shown to exhibit many of the therapeutic properties of MSCs. For example, MSC-derived EVs have demonstrated similar or even superior therapeutic capacity for autoimmune diseases and neurodegenerative disorders compared with their parental MSCs [176, 177]. They also have better safety profiles due to their better immunocompatibility. In addition, they can bypass the endothelial layers in the blood–brain barrier or blood-retinal barrier, providing an ideal cargo to deliver biomolecules to the central nervous system [178].

Several studies have demonstrated the clinical effectiveness of MSC-EVs. For example, hBMMSC-EVs revealed significant improvements in patients suffering from refractory graft-versus-host disease [179]. In another study, administration of hUCMSC-EVs resulted in overall improvement in patients with grade III-IV chronic kidney disease [180]. Nassar et al. conducted a clinical trial to assess the effects of hUCMSC-EVs on pancreatic islet beta cell mass in Type-1 diabetic patients (NCT02138331). And there are other ongoing trials conducted to determine the safety and efficacy of human MSC-EVs in ocular diseases such as promoting the healing of large and refractory macular holes (NCT03437759) and relieving dry eye symptoms in oGVHD patients (NCT04213248). Moreover, MSC-EVs have been modified to load small molecules. For example, miR-124 was loaded in exosomes to treat patients with acute ischemic stroke (NCT03384433).

Advances and perspectives to overcome challenges in MSC clinical application

Artificial intelligence (AI) in MSC treatment

Digital technology and AI are driving the revolution of healthcare industry [181]. The drug research and development became an important application field of AI technology [182]. AI in de novo design has successfully produced biologically active molecules with desired properties [183]. The discovery of drug molecules by AI has been selected as one of the "top ten global breakthrough technologies" by MIT Technology Review in 2020. The advances of AI are likewise expected to boost the understanding of MSCs therapies and help identifying the essential elements of MSCs.

AI can find new molecular compounds and emerging drug targets much faster than traditional methods, thus speeding up the progress of drug development [184, 185]. At the same time, AI can more accurately predict the follow-up experimental results of new drugs, so as to improve the accuracy at each stage of drug development [186]. Computer-aided drug design techniques are thus revolutionizing MSCs therapies.

To understand the essential elements in MSCs treatment, AI may recognize the dynamic molecular characteristics of essential elements, which include different protein sequences, molecular structures, as well as the binding forces and stabilities between targeted molecules and cell receptors. These data could be used to train a predictive model to the utmost accuracy [187]. Predicted elements may also be produced under AI guidance. Powered by a robotic platform, a system developed by MIT researchers partially automates the production of small molecules that could be used in medicine, solar energy, and polymer chemistry. Reportedly, the new system combines three main steps. First, software guided by AI proposes a route for synthesizing a molecule, then chemical experts review this route and refine it into a chemical "recipe," and lastly, the recipe is sent to a robotic platform that automatically assembles the hardware and performs the reactions that build the molecule [188].

At present, the pharmaceutical world is increasingly engaged in technologies to shorten the time required to identify new drugs and repurpose current drugs. Since MSC therapies showed beneficial effects with complex undetermined components, AI may be well-suited to analyzing and revealing essential elements. Companies such as Merck, GSK, and Roche have developed partnerships with AI companies to construct suitable platforms [189, 190]. However, the drug discovery process with AI is a long shot, which need to be verified in clinical trials.

Engineered MSC-EVs for treatment

Paracrine effect was discovered to mediate MSCs therapeutic efficacy in previous studies [191,192,193]. EVs are one of the major paracrine effectors, which are bilayer membrane structures transferring bioactive components [194]. The best-studied EVs can be classified into exosomes and microvesicles according to their sizes, shapes, biogenesis, origins, and compositions [195, 196]. Due to their liposome-like structures reflecting biophysical characteristics of the parental cells, EVs are stable in vivo compared to other foreign particles [197]. Moreover, it is relatively easy to modify and/or improve the contents of EVs and their surface properties to enhance the therapeutic potential or to act as a drug delivery system [198]. These advantages make EVs promising for clinical treatment. Currently, there are 15 clinical trials registered in ClinicalTrial.gov (Table 7). However, none has been completed and challenges remained for the practical application of EVs.

Table 7 The registered clinical trials of treatment using EVs or exosomes derived from MSCs

First of all, the manufacture of large scales of MSC-EVs with high purity is difficult. MSC-EVs are isolated from MSC culture media, of which conditions including the seeding cell number, media volume, and isolation method and time of EVs can influence both the quantity and quality of EVs [199]. Therefore, optimization of culture methods (e.g., hypoxia, sheer stress, and bioreactor) combining with intensive evaluation of the pros and cons of the different EVs isolation methods is prerequisites for MSC-EVs to yield improvements. These procedures should be regulated and controlled to ensure the clinical-grade EVs production [200]. Recently, Mendt et al. reported using a bioreactor system in the GMP facility to obtain sterile, clinical-grade EVs from BM-MSCs. In that instance, the therapeutic effects of BM-MSCs on pancreatic cancer xenograft mouse models were evaluated, and feasible directions for clinical application of MSC-EVs were provided [201].

Safety and efficacy of MSC-EVs in various disease conditions need to be ensured in further preclinical and clinical evaluation. In vivo distribution analysis of fluorescence-labeled EVs has shown that MSC-EVs might have homing capacity for injured or tumor-bearing sites comparable as MSCs [202]. Long-term toxicity and immunogenicity of repetitive EVs administration using hematological examination, histopathological analysis, and immunotyping test should also be performed to find whether MSC-EVs might trigger immune responses or toxic reactions [203].

After the disclosure of precise mechanisms of action or key therapeutic factors in MSC-EVs therapy, targeted-EVs could be expanded in uniform proliferative cells such as fibroblasts via gene modification technology. Therefore, with big data-based analysis of transcriptome and proteome, engineered EVs may be manufactured with desired elements. For instance, Thomas C. Roberts et al. engineered EVs to express IL6 signal transducer (IL6ST) decoy receptors to selectively inhibit the IL6 trans-signaling pathway. Treatment in the Duchenne muscular dystrophy mouse model with these IL6ST decoy receptor EVs resulted in a reduced phosphorylation of STAT3 in muscles; further functional studies verified the in vivo activity of the decoy receptor EVs as a potential therapy [204]. Similarly, CXCR4/TRAIL-enriched exosomes were successfully obtained from MSCs overexpressing both CXCR4 and TRAIL. These exosomes exerted activity as a cooperative agent with carboplatin against brain metastasis of breast cancer in vivo, improving the efficacy of chemotherapy and highlighting a novel synergistic protocol with anticancer agents to treat brain diseases [205, 206]. Moreover, in a Phase 1 clinical trial, IL-12 was engineered to express on the exosome surface using Codiak’s proprietary engEx Platform. This product could enhance the dose control of IL-12 and limit systemic exposure and associated toxicity. EVs can overcome the reported limitations of parental cells on various aspects, including safety, reproducibility, and cost-effectiveness related to storage and maintenance. Engineered EVs might be novel promising therapeutics for clinical application. Furthermore, to resolve current hurdles in EVs-based therapeutics, the production of EVs should be standardized and optimized, and its underlying mechanisms need further investigation.

MSC usage for pandemic diseases such as COVID-19

Pandemic diseases like 2019 novel coronavirus disease (COVID-19) have dramatically increased the number of sickness and death worldwide. Though vaccines have been developed recently, the viruses are still rapidly mutating and expanding, and the available specific and effective treatment options are currently very limited [207]. For severe or critical COVID-19 patients requiring hospitalization, acute lung injures (ALI)/acute respiratory distress syndrome (ARDS) was the main pathologic features, characterized by immunopathological complications with cellular fibromyxoid exudates, extensive pulmonary inflammation, pulmonary edema, and hyaline membrane formation [208]. Besides, inflammation and sepsis are also the leading causes of mortality in COVID-19 patients [209]. In all these cases, any treatment that could hasten recovery would be in substantial demand. MSC therapy may be one such treatment.

MSC therapeutics may be the ideal candidates for handling the broad spectrum of COVID-19 symptoms due to their multifactorial mode-of-action [210]. They can release various factors including keratinocyte growth factor, prostaglandin E2, granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-6, and IL-13 to facilitate the phagocytosis and alternative activation of alveolar macrophages, alter the cytokine secretion profile of dendritic cell subsets, and decrease the release of interferon γ from natural killer cells [211]. For example, IL-10, TGF-β, and tryptophan catabolizing enzyme indoleamine 2,3-dioxygenase secreted from them were reported to suppress the proliferation of T cells and change the cytokine secretion profile of T cell subsets [212]. Moreover, the proliferation, differentiation, and chemotactic properties of B cells were impaired by MSCs as well. Except for the immune regulatory effects, MSCs can enhance the restoration of capillary barriers, inhibit bacterial growth, and restore alveolar ATP. All these functions mentioned above might also be effective in COVID-19 infection.

COVID-19 has been the top priority of global healthcare systems since its emergence. There have been more than 160 vaccines in development and more than 60 clinical trials are ongoing, and now, only a few vaccines have been approved [213]. The representative clinical trials of MSC therapy in COVID-19 disease were listed in Table 8. But the rapid mutation of SARS-CoV-2 virus leads to challenges on the effect of the available vaccine. It is an urgent need to develop more universal and stable therapy to reverse or combat. Though no evidence has showed that coronavirus was eliminated completely after stem cell treatments, preliminary results were promising. Diseased patients were more likely to survive the infection after the treatment. The specific primed MSCs were also investigated for COVID-19 treatment [212, 214]. The results will provide a strong foundation for future scientific research and clinical applications for a variety of diseases including pandemic crisis and pulmonary complications. Hopefully, the approaches utilizing MSCs particularly the primed MSCs could be vital for the success of cell therapy in treating COVID-19.

Table 8 MSCs therapies for COVID-19 in clinical trials

Conclusions

Although MSCs therapies have achieved tremendous advancements over the past decades, substantial challenges remain to be overcome. The main challenges include the immunocompatibility, stability, heterogeneity, differentiation, and migratory capacity. More and more studies are focusing on the attempts to overcome these shortcomings. Although the detailed mechanism of MSCs immunomodulatory effects is still elusive and any attempts to improve MSCs efficacy are still lack of evidence, the preclinical studies are developing rapidly and more standardized clinical trials are wildly carried out. It might be expected that the conversion to canonically registered MSC therapies will flourish with time. The lessons from the current MSCs investigations may provide critical guidance for investigators pursuing further translational processes. With the clarification of MSCs effectors and the emergences of new technologies assisting in-depth studies, MSCs are promising to be proved as effective treatment options for a variety of devastating conditions.

Availability of data and materials

The material supporting the conclusions of this review is included within the article.

Abbreviations

AI:

Artificial intelligence

ALI:

Acute lung injures

ALS:

Amyotrophic lateral sclerosis

ARDS:

Acute respiratory distress syndrome

BMP:

Bone morphogenetic protein

BDNF:

Brain-derived neurotrophic factor

CD:

Crohn’s disease

CM:

Conditioned medium

ECM:

Extracellular matrix

EDA:

Ectodysplasin

EVs:

Extracellular vesicles

FBR:

Foreign-body response

FDA:

Food and Drug Administration

GDNF:

Glial cell line-derived neurotrophic factor

GM-CSF:

Granulocyte–macrophage colony-stimulating factor

GMP:

Good manufacturing practices

GVHD:

Graft-versus-host disease

hBMMSC-EVs:

Extracellular vesicles from human bone marrow-derived mesenchymal stromal cells

HGF:

Hepatocyte growth factor

HLA:

Human leukocyte antigen

hUCMSC-EVs:

Extracellular vesicles from human umbilical cord-derived mesenchymal stromal cells

IDO:

Indoleamine 2,3-dioxygenase

IGF:

Insulin-like growth factor

IL6ST:

IL6 signal transducer

MHC:

Major histocompatibility complex

MI:

Myocardial infarction

MIT:

Massachusetts Institute of Technology

MPs:

Microparticles

MS:

Multiple sclerosis

MSCs:

Mesenchymal stromal cells

MVs:

Microvesicles

NGF:

Nerve growth factor

NTFs:

Neurotrophic factors

oGVHD:

Ocular graft-versus-host disease

PBMCs:

Peripheral blood mononuclear cells

TALEN:

Transcription activator like effector nuclease

Trx1:

Thioredoxin-1

VCAM1:

Vascular cell adhesion molecule-1

VEGF:

Vascular endothelial growth factor

ZFNs:

Zinc-finger nucleases

References

  1. 1.

    Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143–7.

    CAS  Article  Google Scholar 

  2. 2.

    Midha S, Jain KG, Bhaskar N, Kaur A, Rawat S, Giri S, et al. Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs. Stem Cells Transl Med. 2020. https://doi.org/10.1002/sctm.19-0385.

    Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    Vaananen HK. Mesenchymal stem cells. Ann Med. 2005;37(7):469–79.

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  4. 4.

    Ullah I, Subbarao RB, Rho GJ. Human mesenchymal stem cells—current trends and future prospective. Biosci Rep. 2015;35(2):e00191.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  5. 5.

    Reagan MR, Kaplan DL. Concise review: Mesenchymal stem cell tumor-homing: detection methods in disease model systems. Stem Cells. 2011;29(6):920–7.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  6. 6.

    Levy O, Kuai R, Siren EMJ, Bhere D, Milton Y, Nissar N, et al. Shattering barriers toward clinically meaningful MSC therapies. Sci Adv. 2020;6(30):6884.

    Article  CAS  Google Scholar 

  7. 7.

    Martínez-Carrasco R, Sánchez-Abarca LI, Nieto-Gómez C, Martín García E, Sánchez-Guijo F, Argüeso P, et al. Subconjunctival injection of mesenchymal stromal cells protects the cornea in an experimental model of GVHD. Ocul Surf. 2019;17(2):285–94.

    PubMed  Article  PubMed Central  Google Scholar 

  8. 8.

    Petrou P, Gothelf Y, Argov Z, Gotkine M, Levy YS, Kassis I, et al. Safety and clinical effects of mesenchymal stem cells secreting neurotrophic factor transplantation in patients with amyotrophic lateral sclerosis: results of phase 1/2 and 2a clinical trials. JAMA Neurol. 2016;73(3):337–44.

    PubMed  Article  PubMed Central  Google Scholar 

  9. 9.

    Zhao K, Liu Q. The clinical application of mesenchymal stromal cells in hematopoietic stem cell transplantation. J Hematol Oncol. 2016;9(1):46.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  10. 10.

    Park YB, Ha CW, Lee CH, Yoon YC, Park YG. Cartilage regeneration in osteoarthritic patients by a composite of allogeneic umbilical cord blood-derived mesenchymal stem cells and hyaluronate hydrogel: results from a clinical trial for safety and proof-of-concept with 7 years of extended follow-up. Stem Cells Transl Med. 2017;6(2):613–21.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  11. 11.

    Rubin R. Unproven but profitable: the boom in US stem cell clinics. JAMA. 2018;320(14):1421–3.

    PubMed  Article  PubMed Central  Google Scholar 

  12. 12.

    Dimmeler S, Ding S, Rando TA, Trounson A. Translational strategies and challenges in regenerative medicine. Nat Med. 2014;20(8):814–21.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  13. 13.

    Wang S, Qu X, Zhao RC. Clinical applications of mesenchymal stem cells. J Hematol Oncol. 2012;5:19.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  14. 14.

    Conrad C, Niess H, Huss R, Huber S, von Luettichau I, Nelson PJ, et al. Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo. Circulation. 2009;119(2):281–9.

    PubMed  Article  PubMed Central  Google Scholar 

  15. 15.

    Haga H, Yan IK, Takahashi K, Wood J, Zubair A, Patel T. Tumour cell-derived extracellular vesicles interact with mesenchymal stem cells to modulate the microenvironment and enhance cholangiocarcinoma growth. J Extracell Vesicles. 2015;4:24900.

    PubMed  Article  PubMed Central  Google Scholar 

  16. 16.

    Ankrum JA, Ong JF, Karp JM. Mesenchymal stem cells: immune evasive, not immune privileged. Nat Biotechnol. 2014;32(3):252–60.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  17. 17.

    Barrachina L, Remacha AR, Romero A, Vázquez FJ, Albareda J, Prades M, et al. Priming equine bone marrow-derived mesenchymal stem cells with proinflammatory cytokines: implications in immunomodulation-immunogenicity balance, cell viability, and differentiation potential. Stem Cells Dev. 2017;26(1):15–24.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  18. 18.

    Chan JL, Tang KC, Patel AP, Bonilla LM, Pierobon N, Ponzio NM, et al. Antigen-presenting property of mesenchymal stem cells occurs during a narrow window at low levels of interferon-gamma. Blood. 2006;107(12):4817–24.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  19. 19.

    Skrahin A, Ahmed RK, Ferrara G, Rane L, Poiret T, Isaikina Y, et al. Autologous mesenchymal stromal cell infusion as adjunct treatment in patients with multidrug and extensively drug-resistant tuberculosis: an open-label phase 1 safety trial. Lancet Respir Med. 2014;2(2):108–22.

    PubMed  Article  PubMed Central  Google Scholar 

  20. 20.

    Joswig AJ, Mitchell A, Cummings KJ, Levine GJ, Gregory CA, Smith R 3rd, et al. Repeated intra-articular injection of allogeneic mesenchymal stem cells causes an adverse response compared to autologous cells in the equine model. Stem Cell Res Ther. 2017;8(1):42.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  21. 21.

    Rowland AL, Xu JJ, Joswig AJ, Gregory CA, Antczak DF, Cummings KJ, et al. In vitro MSC function is related to clinical reaction in vivo. Stem Cell Res Ther. 2018;9(1):295.

    PubMed  PubMed Central  Article  Google Scholar 

  22. 22.

    Xia X, Chan KF, Wong GTY, Wang P, Liu L, Yeung BPM, et al. Mesenchymal stem cells promote healing of nonsteroidal anti-inflammatory drug-related peptic ulcer through paracrine actions in pigs. Sci Transl Med. 2019;11(516):eaat7455.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  23. 23.

    Ciuffreda MC, Malpasso G, Musarò P, Turco V, Gnecchi M. Protocols for in vitro differentiation of human mesenchymal stem cells into osteogenic, chondrogenic and adipogenic lineages. Methods Mol Biol. 2016;1416:149–58.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  24. 24.

    Čamernik K, Zupan J. Complete assessment of multilineage differentiation potential of human skeletal muscle-derived mesenchymal stem/stromal cells. Methods Mol Biol. 2019;2045:131–44.

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  25. 25.

    Haraszti RA, Didiot MC, Sapp E, Leszyk J, Shaffer SA, Rockwell HE, et al. High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources. J Extracell Vesicles. 2016;5:32570.

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  26. 26.

    Wang X, Shah FA, Vazirisani F, Johansson A, Palmquist A, Omar O, et al. Exosomes influence the behavior of human mesenchymal stem cells on titanium surfaces. Biomaterials. 2020;230:119571.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  27. 27.

    Jozkowiak M, Hutchings G, Jankowski M, Kulcenty K, Mozdziak P, Kempisty B, et al. The stemness of human ovarian granulosa cells and the role of resveratrol in the differentiation of MSCs-A review based on cellular and molecular knowledge. Cells. 2020;9(6):1418.

    CAS  PubMed Central  Article  Google Scholar 

  28. 28.

    Lin GL, Hankenson KD. Integration of BMP, Wnt, and notch signaling pathways in osteoblast differentiation. J Cell Biochem. 2011;112(12):3491–501.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  29. 29.

    Lu GM, Rong YX, Liang ZJ, Hunag DL, Ma YF, Luo ZZ, et al. Multiomics global landscape of stemness-related gene clusters in adipose-derived mesenchymal stem cells. Stem Cell Res Ther. 2020;11(1):310.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  30. 30.

    Tao X, Sun MY, Chen M, Ying RC, Su WJ, Zhang J, et al. HMGB1-modified mesenchymal stem cells attenuate radiation-induced vascular injury possibly via their high motility and facilitation of endothelial differentiation. Stem Cell Res Ther. 2019;10(1):92.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  31. 31.

    Wang HM, Zhou Y, Yu D, Zhu HY. Klf2 contributes to the stemness and self-renewal of human bone marrow stromal cells. Cytotechnology. 2016;68(4):839–48.

    CAS  PubMed  Article  Google Scholar 

  32. 32.

    Choi MR, In YH, Park J, Park T, Jung KH, Chai JC, et al. Genome-scale DNA methylation pattern profiling of human bone marrow mesenchymal stem cells in long-term culture. Exp Mol Med. 2012;44(8):503–12.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  33. 33.

    Murphy M. Delayed early embryonic lethality following disruption of the murine cyclin A2 gene. Nat Genet. 1997;15(1):83–6.

    CAS  PubMed  Article  Google Scholar 

  34. 34.

    Baple EL, Chambers H, Cross HE, Fawcett H, Nakazawa Y, Chioza BA, et al. Hypomorphic PCNA mutation underlies a human DNA repair disorder. J Clin Invest. 2014;124(7):3137–46.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  35. 35.

    Toukoki C, Gryllos I. PolA1, a Putative DNA Polymerase I, Is coexpressed with PerR and contributes to peroxide stress defenses of group A streptococcus. J Bacteriol. 2013;195(4):717–25.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  36. 36.

    Palles C, Cazier JB, Howarth KM, Domingo E, Jones AM, Broderick P, et al. Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas. Nat Genet. 2013;45(2):136–44.

    CAS  PubMed  Article  Google Scholar 

  37. 37.

    Xiang J, Fang LK, Luo YX, Yang ZL, Liao Y, Cui J, et al. Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer. J Transl Med. 2014;12:320.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  38. 38.

    Li Y, Benezra R. Identification of a human mitotic checkpoint gene: hsMAD2. Science. 1996;274(5285):246–8.

    CAS  PubMed  Article  Google Scholar 

  39. 39.

    Al Jord A, Shihavuddin A, Servignat d’Aout R, Faucourt M, Genovesio A, Karaiskou A, et al. Calibrated mitotic oscillator drives motile ciliogenesis. Science. 2017;358(6364):803–6.

    CAS  PubMed  Article  Google Scholar 

  40. 40.

    Gong D, Ferrell JE. The roles of cyclin A2, B1, and B2 in early and late mitotic events. Mol Biol Cell. 2010;21(18):3149–61.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  41. 41.

    Fenwick AL, Kliszczak M, Cooper F, Murray J, Sanchez-Pulido L, Twigg SRF, et al. Mutations in CDC45, encoding an essential component of the pre-initiation complex, cause meier-gorlin syndrome and craniosynostosis. Am J Hum Genet. 2016;99(1):125–38.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  42. 42.

    Aiken J, Moore JK, Bates EA. TUBA1A mutations identified in lissencephaly patients dominantly disrupt neuronal migration and impair dynein activity. Hum Mol Genet. 2019;28(8):1227–43.

    CAS  PubMed  Article  Google Scholar 

  43. 43.

    Morris EJ, Ji JY, Yang FJ, Di Stefano L, Herr A, Moon NS, et al. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature. 2008;455(7212):552–6.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  44. 44.

    Caldas H, Holloway MP, Hall BM, Qualman SJ, Altura RA. Survivin-directed RNA interference cocktail is a potent suppressor of tumour growth in vivo. J Med Genet. 2006;43(2):119–28.

    CAS  PubMed  Article  Google Scholar 

  45. 45.

    Wang YB, Li S, Smith K, Waldman BC, Waldman AS. Intrachromosomal recombination between highly diverged DNA sequences is enabled in human cells deficient in Bloom helicase. DNA Repair (Amst). 2016;41:73–84.

    Article  CAS  Google Scholar 

  46. 46.

    Kim H, Wrann CD, Jedrychowski M, Vidoni S, Kitase Y, Nagano K, et al. Irisin mediates effects on bone and fat via αV integrin receptors. Cell. 2018;175(7):1756–68.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  47. 47.

    Pan SH, Tai CC, Lin CS, Hsu WB, Chou SF, Lai CC, et al. Epstein-Barr virus nuclear antigen 2 disrupts mitotic checkpoint and causes chromosomal instability. Carcinogenesis. 2009;30(2):366–75.

    CAS  PubMed  Article  Google Scholar 

  48. 48.

    Ng CP, Sharif AR, Heath DE, Chow JW, Zhang CB, Chan-Park MB, et al. Enhanced ex vivo expansion of adult mesenchymal stem cells by fetal mesenchymal stem cell ECM. Biomaterials. 2014;35(13):4046–57.

    CAS  PubMed  Article  Google Scholar 

  49. 49.

    Zhang L, Mack R, Breslin P, Zhang J. Molecular and cellular mechanisms of aging in hematopoietic stem cells and their niches. J Hematol Oncol. 2020;13(1):157.

    PubMed  PubMed Central  Article  Google Scholar 

  50. 50.

    Cao H, Xiao J, Reeves ME, Payne K, Chen CS, Baylink DJ, et al. Discovery of proangiogenic CD44+mesenchymal cancer stem cells in an acute myeloid leukemia patient’s bone marrow. J Hematol Oncol. 2020;13(1):63.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  51. 51.

    Kim JH, Shin SH, Li TZ, Suh H. Influence of in vitro biomimicked stem cell “niche” for regulation of proliferation and differentiation of human bone marrow-derived mesenchymal stem cells to myocardial phenotypes: serum starvation without aid of chemical agents and prevention of spontaneous stem cell transformation enhanced by the matrix environment. J Tissue Eng Regen Med. 2016;10(1):E1-13.

    CAS  PubMed  Article  Google Scholar 

  52. 52.

    Rodriguez R, Rubio R, Masip M, Catalina P, Nieto A, de la Cueva T, et al. Loss of p53 induces tumorigenesis in p21-deficient mesenchymal stem cells. Neoplasia. 2009;11(4):397–407.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  53. 53.

    McLean K, Gong Y, Choi Y, Deng N, Yang K, Bai S, et al. Human ovarian carcinoma-associated mesenchymal stem cells regulate cancer stem cells and tumorigenesis via altered BMP production. J Clin Invest. 2011;121(8):3206–19.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  54. 54.

    Direkze NC, Hodivala-Dilke K, Jeffery R, Hunt T, Poulsom R, Oukrif D, et al. Bone marrow contribution to tumor-associated myofibroblasts and fibroblasts. Cancer Res. 2004;64(23):8492–5.

    CAS  PubMed  Article  Google Scholar 

  55. 55.

    Yoshida GJ, Azuma A, Miura Y, Orimo A. Activated fibroblast program orchestrates tumor initiation and progression; molecular mechanisms and the associated therapeutic strategies. Int J Mol Sci. 2019;20(9):2256.

    CAS  PubMed Central  Article  PubMed  Google Scholar 

  56. 56.

    Jeon ES, Moon HJ, Lee MJ, Song HY, Kim YM, Cho M, et al. Cancer-derived lysophosphatidic acid stimulates differentiation of human mesenchymal stem cells to myofibroblast-like cells. Stem Cells. 2008;26(3):789–97.

    CAS  PubMed  Article  Google Scholar 

  57. 57.

    Yoshida GJ. Regulation of heterogeneous cancer-associated fibroblasts: the molecular pathology of activated signaling pathways. J Exp Clin Cancer Res. 2020;39(1):112.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  58. 58.

    Maeda K, Enomoto A, Hara A, Asai N, Kobayashi T, Horinouchi A, et al. Identification of meflin as a potential marker for mesenchymal stromal cells. Sci Rep. 2016;6:22288.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  59. 59.

    Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, et al. Meflin-positive cancer-associated fibroblasts inhibit pancreatic carcinogenesis. Cancer Res. 2019;79(20):5367–81.

    CAS  PubMed  Article  Google Scholar 

  60. 60.

    Li X, Zeng X, Xu Y, Wang B, Zhao Y, Lai X, et al. Mechanisms and rejuvenation strategies for aged hematopoietic stem cells. J Hematol Oncol. 2020;13(1):31.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  61. 61.

    Gallina C, Capelôa T, Saviozzi S, Accomasso L, Catalano F, Tullio F, et al. Human mesenchymal stem cells labelled with dye-loaded amorphous silica nanoparticles: long-term biosafety, stemness preservation and traceability in the beating heart. J Nanobiotechnol. 2015;13:77.

    Article  CAS  Google Scholar 

  62. 62.

    Nicolay NH, Lopez Perez R, Debus J, Huber PE. Mesenchymal stem cells—a new hope for radiotherapy-induced tissue damage? Cancer Lett. 2015;366(2):133–40.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  63. 63.

    Fong CY, Subramanian A, Biswas A, Bongso A. Freezing of fresh Wharton’s jelly from human umbilical cords yields high post-thaw mesenchymal stem cell numbers for cell-based therapies. J Cell Biochem. 2016;117(4):815–27.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  64. 64.

    Yin Z, Dong C, Jiang K, Xu Z, Li R, Guo K, et al. Heterogeneity of cancer-associated fibroblasts and roles in the progression, prognosis, and therapy of hepatocellular carcinoma. J Hematol Oncol. 2019;12(1):101.

    PubMed  PubMed Central  Article  Google Scholar 

  65. 65.

    Tallone T, Realini C, Böhmler A, Kornfeld C, Vassalli G, Moccetti T, et al. Adult human adipose tissue contains several types of multipotent cells. J Cardiovasc Transl Res. 2011;4(2):200–10.

    PubMed  Article  PubMed Central  Google Scholar 

  66. 66.

    Andrzejewska A, Dabrowska S, Nowak B, Walczak P, Lukomska B, Janowski M. Mesenchymal stem cells injected into carotid artery to target focal brain injury home to perivascular space. Theranostics. 2020;10(15):6615–28.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  67. 67.

    Ducret M, Farges JC, Pasdeloup M, Perrier-Groult E, Mueller A, Mallein-Gerin F, et al. Phenotypic identification of dental pulp mesenchymal stem/stromal cells subpopulations with multiparametric flow cytometry. Methods Mol Biol. 2019;1922:77–90.

    CAS  PubMed  Article  Google Scholar 

  68. 68.

    Yang H, Gao LN, An Y, Hu CH, Jin F, Zhou J, et al. Comparison of mesenchymal stem cells derived from gingival tissue and periodontal ligament in different incubation conditions. Biomaterials. 2013;34(29):7033–47.

    CAS  PubMed  Article  Google Scholar 

  69. 69.

    Choi YS, Park SN, Suh H. Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres. Biomaterials. 2005;26(29):5855–63.

    CAS  PubMed  Article  Google Scholar 

  70. 70.

    Holan V, Trosan P, Cejka C, Javorkova E, Zajicova A, Hermankova B, et al. A comparative study of the therapeutic potential of mesenchymal stem cells and limbal epithelial stem cells for ocular surface reconstruction. Stem Cells Transl Med. 2015;4(9):1052–63.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  71. 71.

    Roura S, Farré J, Soler-Botija C, Llach A, Hove-Madsen L, Cairó JJ, et al. Effect of aging on the pluripotential capacity of human CD105+ mesenchymal stem cells. Eur J Heart Fail. 2006;8(6):555–63.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  72. 72.

    Pogozhykh O, Pogozhykh D, Neehus AL, Hoffmann A, Blasczyk R, Müller T. Molecular and cellular characteristics of human and non-human primate multipotent stromal cells from the amnion and bone marrow during long term culture. Stem Cell Res Ther. 2015;6(1):150.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  73. 73.

    Sarkar D, Spencer JA, Phillips JA, Zhao W, Schafer S, Spelke DP, et al. Engineered cell homing. Blood. 2011;118(25):e184-191.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  74. 74.

    Won YW, Patel AN, Bull DA. Cell surface engineering to enhance mesenchymal stem cell migration toward an SDF-1 gradient. Biomaterials. 2014;35(21):5627–35.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  75. 75.

    Pallares V, Unzueta U, Falgas A, Sanchez-Garcia L, Serna N, Gallardo A, et al. An Auristatin nanoconjugate targeting CXCR4+ leukemic cells blocks acute myeloid leukemia dissemination. J Hematol Oncol. 2020;13(1):36.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  76. 76.

    Huang J, Zhang Z, Guo J, Ni A, Deb A, Zhang L, et al. Genetic modification of mesenchymal stem cells overexpressing CCR1 increases cell viability, migration, engraftment, and capillary density in the injured myocardium. Circ Res. 2010;106(11):1753–62.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  77. 77.

    Li H, Jiang Y, Jiang X, Guo X, Ning H, Li Y, et al. CCR7 guides migration of mesenchymal stem cell to secondary lymphoid organs: a novel approach to separate GvHD from GvL effect. Stem Cells. 2014;32(7):1890–903.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  78. 78.

    Robles JD, Liu YP, Cao J, Xiang Z, Cai Y, Manio M, et al. Immunosuppressive mechanisms of human bone marrow derived mesenchymal stromal cells in BALB/c host graft versus host disease murine models. Exp Hematol Oncol. 2015;4:13.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  79. 79.

    Zhang X, Huang W, Chen X, Lian Y, Wang J, Cai C, et al. CXCR5-overexpressing mesenchymal stromal cells exhibit enhanced homing and can decrease contact hypersensitivity. Mol Ther. 2017;25(6):1434–47.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  80. 80.

    Liu Z, Mikrani R, Zubair HM, Taleb A, Naveed M, Baig M, et al. Systemic and local delivery of mesenchymal stem cells for heart renovation: challenges and innovations. Eur J Pharmacol. 2020;876:173049.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  81. 81.

    Li L, Dong L, Zhang J, Gao F, Hui J, Yan J. Mesenchymal stem cells with downregulated Hippo signaling attenuate lung injury in mice with lipopolysaccharide-induced acute respiratory distress syndrome. Int J Mol Med. 2019;43(3):1241–52.

    CAS  PubMed  PubMed Central  Google Scholar 

  82. 82.

    Dick AJ, Guttman MA, Raman VK, Peters DC, Pessanha BS, Hill JM, et al. Magnetic resonance fluoroscopy allows targeted delivery of mesenchymal stem cells to infarct borders in Swine. Circulation. 2003;108(23):2899–904.

    PubMed  PubMed Central  Article  Google Scholar 

  83. 83.

    Monsanto MM, Wang BJ, Ehrenberg ZR, Echeagaray O, White KS, Alvarez R Jr, et al. Enhancing myocardial repair with CardioClusters. Nat Commun. 2020;11(1):3955.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  84. 84.

    Srivastava AK, Bulte CA, Shats I, Walczak P, Bulte JW. Co-transplantation of syngeneic mesenchymal stem cells improves survival of allogeneic glial-restricted precursors in mouse brain. Exp Neurol. 2016;275 Pt 1((0-1)):154–61.

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  85. 85.

    Neuhuber B, Swanger SA, Howard L, Mackay A, Fischer I. Effects of plating density and culture time on bone marrow stromal cell characteristics. Exp Hematol. 2008;36(9):1176–85.

    PubMed  PubMed Central  Article  Google Scholar 

  86. 86.

    Colter DC, Class R, DiGirolamo CM, Prockop DJ. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci U S A. 2000;97(7):3213–8.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  87. 87.

    Zhao Q, Zhang L, Wei Y, Yu H, Zou L, Huo J, et al. Systematic comparison of hUC-MSCs at various passages reveals the variations of signatures and therapeutic effect on acute graft-versus-host disease. Stem Cell Res Ther. 2019;10(1):354.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  88. 88.

    Ryan AE, Lohan P, O’Flynn L, Treacy O, Chen X, Coleman C, et al. Chondrogenic differentiation increases antidonor immune response to allogeneic mesenchymal stem cell transplantation. Mol Ther. 2014;22(3):655–67.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  89. 89.

    Nekouian S, Sojoodi M, Nadri S. Fabrication of conductive fibrous scaffold for photoreceptor differentiation of mesenchymal stem cell. J Cell Physiol. 2019. https://doi.org/10.1002/jcp.28238.

    Article  PubMed  PubMed Central  Google Scholar 

  90. 90.

    Khan AA, Huat TJ, Al Mutery A, El-Serafi AT, Kacem HH, Abdallah SH, et al. Significant transcriptomic changes are associated with differentiation of bone marrow-derived mesenchymal stem cells into neural progenitor-like cells in the presence of bFGF and EGF. Cell Biosci. 2020;10:126.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  91. 91.

    Venkatesh K, Sen D. Mesenchymal stem cells as a source of dopaminergic neurons: a potential cell based therapy for Parkinson’s disease. Curr Stem Cell Res Ther. 2017;12(4):326–47.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  92. 92.

    Gnecchi M, He H, Noiseux N, Liang OD, Zhang L, Morello F, et al. Evidence supporting paracrine hypothesis for Akt-modified mesenchymal stem cell-mediated cardiac protection and functional improvement. FASEB J. 2006;20(6):661–9.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  93. 93.

    Dai W, Hale SL, Kloner RA. Role of a paracrine action of mesenchymal stem cells in the improvement of left ventricular function after coronary artery occlusion in rats. Regen Med. 2007;2(1):63–8.

    PubMed  Article  PubMed Central  Google Scholar 

  94. 94.

    Ko HR, Ahn SY, Chang YS, Hwang I, Yun T, Sung DK, et al. Human UCB-MSCs treatment upon intraventricular hemorrhage contributes to attenuate hippocampal neuron loss and circuit damage through BDNF-CREB signaling. Stem Cell Res Ther. 2018;9(1):326.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  95. 95.

    Whone AL, Kemp K, Sun M, Wilkins A, Scolding NJ. Human bone marrow mesenchymal stem cells protect catecholaminergic and serotonergic neuronal perikarya and transporter function from oxidative stress by the secretion of glial-derived neurotrophic factor. Brain Res. 2012;1431:86–96.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  96. 96.

    Silva M, Monteiro GA, Fialho AM, Bernardes N, da Silva CL. Conditioned medium from azurin-expressing human mesenchymal stromal cells demonstrates antitumor activity against breast and lung cancer cell lines. Front Cell Dev Biol. 2020;8:471.

    PubMed  PubMed Central  Article  Google Scholar 

  97. 97.

    Guo L, Lai P, Wang Y, Huang T, Chen X, Geng S, et al. Extracellular vesicles derived from mesenchymal stem cells prevent skin fibrosis in the cGVHD mouse model by suppressing the activation of macrophages and B cells immune response. Int Immunopharmacol. 2020;84:106541.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  98. 98.

    Lai P, Weng J, Guo L, Chen X, Du X. Novel insights into MSC-EVs therapy for immune diseases. Biomark Res. 2019;7:6.

    PubMed  PubMed Central  Article  Google Scholar 

  99. 99.

    Guo L, Lai P, Wang Y, Huang T, Chen X, Luo C, et al. Extracellular vesicles from mesenchymal stem cells prevent contact hypersensitivity through the suppression of Tc1 and Th1 cells and expansion of regulatory T cells. Int Immunopharmacol. 2019;74:105663.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  100. 100.

    Wang W, Han Y, Jo HA, Lee J, Song YS. Non-coding RNAs shuttled via exosomes reshape the hypoxic tumor microenvironment. J Hematol Oncol. 2020;13(1):67.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  101. 101.

    Rostom DM, Attia N, Khalifa HM, Abou Nazel MW, El Sabaawy EA. The therapeutic potential of extracellular vesicles versus mesenchymal stem cells in liver damage. Tissue Eng Regen Med. 2020;17(4):537–52.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  102. 102.

    Swartzlander MD, Blakney AK, Amer LD, Hankenson KD, Kyriakides TR, Bryant SJ. Immunomodulation by mesenchymal stem cells combats the foreign body response to cell-laden synthetic hydrogels. Biomaterials. 2015;41:79–88.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  103. 103.

    Tzouanas SN, Ekenseair AK, Kasper FK, Mikos AG. Mesenchymal stem cell and gelatin microparticle encapsulation in thermally and chemically gelling injectable hydrogels for tissue engineering. J Biomed Mater Res A. 2014;102(5):1222–30.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  104. 104.

    Zhang X, Yang Y, Zhang L, Lu Y, Zhang Q, Fan D, et al. Mesenchymal stromal cells as vehicles of tetravalent bispecific Tandab (CD3/CD19) for the treatment of B cell lymphoma combined with IDO pathway inhibitor D-1-methyl-tryptophan. J Hematol Oncol. 2017;10(1):56.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  105. 105.

    Ankrum JA, Dastidar RG, Ong JF, Levy O, Karp JM. Performance-enhanced mesenchymal stem cells via intracellular delivery of steroids. Sci Rep. 2014;4:4645.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  106. 106.

    Luo L, Tang J, Nishi K, Yan C, Dinh PU, Cores J, et al. Fabrication of synthetic mesenchymal stem cells for the treatment of acute myocardial infarction in mice. Circ Res. 2017;120(11):1768–75.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  107. 107.

    Valles G, Bensiamar F, Crespo L, Arruebo M, Vilaboa N, Saldana L. Topographical cues regulate the crosstalk between MSCs and macrophages. Biomaterials. 2015;37:124–33.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  108. 108.

    Stucky EC, Schloss RS, Yarmush ML, Shreiber DI. Alginate micro-encapsulation of mesenchymal stromal cells enhances modulation of the neuro-inflammatory response. Cytotherapy. 2015;17(10):1353–64.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  109. 109.

    Follin B, Juhl M, Cohen S, Pedersen AE, Gad M, Kastrup J, et al. Human adipose-derived stromal cells in a clinically applicable injectable alginate hydrogel: phenotypic and immunomodulatory evaluation. Cytotherapy. 2015;17(8):1104–18.

    CAS  PubMed  Article  Google Scholar 

  110. 110.

    Li LM, Han M, Jiang XC, Yin XZ, Chen F, Zhang TY, et al. Peptide-tethered hydrogel scaffold promotes recovery from spinal cord transection via synergism with mesenchymal stem cells. ACS Appl Mater Interfaces. 2017;9(4):3330–42.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  111. 111.

    Murphy KC, Whitehead J, Zhou D, Ho SS, Leach JK. Engineering fibrin hydrogels to promote the wound healing potential of mesenchymal stem cell spheroids. Acta Biomater. 2017;64:176–86.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  112. 112.

    Su N, Gao PL, Wang K, Wang JY, Zhong Y, Luo Y. Fibrous scaffolds potentiate the paracrine function of mesenchymal stem cells: a new dimension in cell-material interaction. Biomaterials. 2017;141:74–85.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  113. 113.

    Wan S, Fu X, Ji Y, Li M, Shi X, Wang Y. FAK- and YAP/TAZ dependent mechanotransduction pathways are required for enhanced immunomodulatory properties of adipose-derived mesenchymal stem cells induced by aligned fibrous scaffolds. Biomaterials. 2018;171:107–17.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  114. 114.

    Olivares-Navarrete R, Hyzy SL, Slosar PJ, Schneider JM, Schwartz Z, Boyan BD. Implant materials generate different peri-implant inflammatory factors: poly-ether-ether-ketone promotes fibrosis and microtextured titanium promotes osteogenic factors. Spine (Phila Pa 1976). 2015;40(6):399–404.

    Article  Google Scholar 

  115. 115.

    Zhu Y, Zhang K, Zhao R, Ye X, Chen X, Xiao Z, et al. Bone regeneration with micro/nano hybrid-structured biphasic calcium phosphate bioceramics at segmental bone defect and the induced immunoregulation of MSCs. Biomaterials. 2017;147:133–44.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  116. 116.

    Roger Y, Schack LM, Koroleva A, Noack S, Kurselis K, Krettek C, et al. Grid-like surface structures in thermoplastic polyurethane induce anti-inflammatory and anti-fibrotic processes in bone marrow-derived mesenchymal stem cells. Colloids Surf B Biointerfaces. 2016;148:104–15.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  117. 117.

    Gomez-Aristizabal A, Kim KP, Viswanathan S. A systematic study of the effect of different molecular weights of hyaluronic acid on mesenchymal stromal cell-mediated immunomodulation. PLoS ONE. 2016;11(1):e0147868.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  118. 118.

    Yuan T, Li K, Guo L, Fan H, Zhang X. Modulation of immunological properties of allogeneic mesenchymal stem cells by collagen scaffolds in cartilage tissue engineering. J Biomed Mater Res A. 2011;98(3):332–41.

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  119. 119.

    Yang Y, Zhang X, Lin F, Xiong M, Fan D, Yuan X, et al. Bispecific CD3-HAC carried by E1A-engineered mesenchymal stromal cells against metastatic breast cancer by blocking PD-L1 and activating T cells. J Hematol Oncol. 2019;12(1):46.

    PubMed  PubMed Central  Article  Google Scholar 

  120. 120.

    Suresh SC, Selvaraju V, Thirunavukkarasu M, Goldman JW, Husain A, Alexander Palesty J, et al. Thioredoxin-1 (Trx1) engineered mesenchymal stem cell therapy increased pro-angiogenic factors, reduced fibrosis and improved heart function in the infarcted rat myocardium. Int J Cardiol. 2015;201:517–28.

    PubMed  Article  PubMed Central  Google Scholar 

  121. 121.

    Gao P, Ding Q, Wu Z, Jiang H, Fang Z. Therapeutic potential of human mesenchymal stem cells producing IL-12 in a mouse xenograft model of renal cell carcinoma. Cancer Lett. 2010;290(2):157–66.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  122. 122.

    Han J, Zhao J, Xu J, Wen Y. Mesenchymal stem cells genetically modified by lentivirus-mediated interleukin-12 inhibit malignant ascites in mice. Exp Ther Med. 2014;8(4):1330–4.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  123. 123.

    Relation T, Yi T, Guess AJ, La Perle K, Otsuru S, Hasgur S, et al. Intratumoral delivery of interferongamma-secreting mesenchymal stromal cells repolarizes tumor-associated macrophages and suppresses neuroblastoma proliferation in vivo. Stem Cells. 2018;36(6):915–24.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  124. 124.

    Seo SH, Kim KS, Park SH, Suh YS, Kim SJ, Jeun SS, et al. The effects of mesenchymal stem cells injected via different routes on modified IL-12-mediated antitumor activity. Gene Ther. 2011;18(5):488–95.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  125. 125.

    Cheng S, Nethi SK, Rathi S, Layek B, Prabha S. Engineered mesenchymal stem cells for targeting solid tumors: therapeutic potential beyond regenerative therapy. J Pharmacol Exp Ther. 2019;370(2):231–41.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  126. 126.

    Mingozzi F, High KA. Immune responses to AAV vectors: overcoming barriers to successful gene therapy. Blood. 2013;122(1):23–36.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  127. 127.

    Wang J, Liu X, Qiu Y, Shi Y, Cai J, Wang B, et al. Cell adhesion-mediated mitochondria transfer contributes to mesenchymal stem cell-induced chemoresistance on T cell acute lymphoblastic leukemia cells. J Hematol Oncol. 2018;11(1):11.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  128. 128.

    Zhao QG, Gregory CA, Lee RH, Reger RL, Qin LZ, Hai B, et al. MSCs derived from iPSCs with a modified protocol are tumor-tropic but have much less potential to promote tumors than bone marrow MSCs. Proc Natl Acad Sci U S A. 2015;112(2):530–5.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  129. 129.

    Gerace D, Martiniello-Wilks R, Nassif NT, Lal S, Steptoe R, Simpson AM. CRISPR-targeted genome editing of mesenchymal stem cell-derived therapies for type 1 diabetes: a path to clinical success? Stem Cell Res Ther. 2017;8(1):62.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  130. 130.

    Faulkner J, Jiang P, Farris D, Walker R, Dai Z. CRISPR/CAS9-mediated knockout of Abi1 inhibits p185(Bcr-Abl)-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways. J Hematol Oncol. 2020;13(1):34.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  131. 131.

    Golchin A, Shams F, Karami F. Advancing mesenchymal stem cell therapy with CRISPR/Cas9 for clinical trial studies. Adv Exp Med Biol. 2020;1247:89–100.

    PubMed  Article  PubMed Central  Google Scholar 

  132. 132.

    Miwa H, Era T. Tracing the destiny of mesenchymal stem cells from embryo to adult bone marrow and white adipose tissue via Pdgfralpha expression. Development. 2018;145(2):dev155879.

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  133. 133.

    von Einem JC, Guenther C, Volk HD, Grutz G, Hirsch D, Salat C, et al. Treatment of advanced gastrointestinal cancer with genetically modified autologous mesenchymal stem cells: results from the phase 1/2 TREAT-ME-1 trial. Int J Cancer. 2019;145(6):1538–46.

    Article  CAS  Google Scholar 

  134. 134.

    Lee S, Kim OJ, Lee KO, Jung H, Oh SH, Kim NK. Enhancing the therapeutic potential of CCL2-overexpressing mesenchymal stem cells in acute stroke. Int J Mol Sci. 2020;21(20):7795.

    CAS  PubMed Central  Article  Google Scholar 

  135. 135.

    Guo XR, Hu QY, Yuan YH, Tang XJ, Yang ZS, Zou DD, et al. PTEN-mRNA engineered mesenchymal stem cell-mediated cytotoxic effects on U251 glioma cells. Oncol Lett. 2016;11(4):2733–40.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  136. 136.

    Hu X, Li L, Yu X, Zhang R, Yan S, Zeng Z, et al. CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs). Oncotarget. 2017;8(67):111847–65.

    PubMed  PubMed Central  Article  Google Scholar 

  137. 137.

    Sun S, Xiao J, Huo J, Geng Z, Ma K, Sun X, et al. Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells. Stem Cell Res Ther. 2018;9(1):8.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  138. 138.

    Meng X, Zheng M, Yu M, Bai W, Zuo L, Bu X, et al. Transplantation of CRISPRa system engineered IL10-overexpressing bone marrow-derived mesenchymal stem cells for the treatment of myocardial infarction in diabetic mice. J Biol Eng. 2019;13:49.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  139. 139.

    Su DN, Wu SP, Xu SZ. Mesenchymal stem cell-based Smad7 gene therapy for experimental liver cirrhosis. Stem Cell Res Ther. 2020;11(1):395.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  140. 140.

    Li SJ, Luo Y, Zhang LM, Yang W, Zhang GG. Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells. Mol Med Rep. 2017;15(3):1313–8.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  141. 141.

    Lee MH, Wu X, Zhu Y. RNA-binding protein PUM2 regulates mesenchymal stem cell fate via repression of JAK2 and RUNX2 mRNAs. J Cell Physiol. 2020;235(4):3874–85.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  142. 142.

    Yin X, Hu L, Zhang Y, Zhu C, Cheng H, Xie X, et al. PDGFB-expressing mesenchymal stem cells improve human hematopoietic stem cell engraftment in immunodeficient mice. Bone Marrow Transplant. 2020;55(6):1029–40.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  143. 143.

    Marina RJ, Brannan KW, Dong KD, Yee BA, Yeo GW. Evaluation of engineered CRISPR-Cas-mediated systems for site-specific RNA editing. Cell Rep. 2020;33(5):108350.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  144. 144.

    Carvalho JL, Braga VB, Melo MB, Campos AC, Oliveira MS, Gomes DA, et al. Priming mesenchymal stem cells boosts stem cell therapy to treat myocardial infarction. J Cell Mol Med. 2013;17(5):617–25.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  145. 145.

    Noronha NC, Mizukami A, Caliari-Oliveira C, Cominal JG, Rocha JLM, Covas DT, et al. Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies. Stem Cell Res Ther. 2019;10(1):131.

    PubMed  Article  PubMed Central  Google Scholar 

  146. 146.

    Kim DS, Jang IK, Lee MW, Ko YJ, Lee DH, Lee JW, et al. Enhanced immunosuppressive properties of human mesenchymal stem cells primed by interferon-gamma. EBioMedicine. 2018;28:261–73.

    PubMed  PubMed Central  Article  Google Scholar 

  147. 147.

    Mead B, Chamling X, Zack DJ, Ahmed Z, Tomarev S. TNFalpha-mediated priming of mesenchymal stem cells enhances their neuroprotective effect on retinal ganglion cells. Invest Ophthalmol Vis Sci. 2020;61(2):6.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  148. 148.

    Chinnadurai R, Copland IB, Patel SR, Galipeau J. IDO-independent suppression of T cell effector function by IFN-gamma-licensed human mesenchymal stromal cells. J Immunol. 2014;192(4):1491–501.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  149. 149.

    Rovira Gonzalez YI, Lynch PJ, Thompson EE, Stultz BG, Hursh DA. In vitro cytokine licensing induces persistent permissive chromatin at the Indoleamine 2,3-dioxygenase promoter. Cytotherapy. 2016;18(9):1114–28.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  150. 150.

    Takeshita K, Motoike S, Kajiya M, Komatsu N, Takewaki M, Ouhara K, et al. Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration. Stem Cell Res Ther. 2017;8(1):101.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  151. 151.

    Jo H, Eom YW, Kim HS, Park HJ, Kim HM, Cho MY. Regulatory dendritic cells induced by mesenchymal stem cells ameliorate dextran sodium sulfate-induced chronic colitis in mice. Gut Liver. 2018;12(6):664–73.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  152. 152.

    Hu CD, Kosaka Y, Marcus P, Rashedi I, Keating A. Differential immunomodulatory effects of human bone marrow-derived mesenchymal stromal cells on natural killer cells. Stem Cells Dev. 2019;28(14):933–43.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  153. 153.

    Ma ZJ, Wang YH, Li ZG, Wang Y, Li BY, Kang HY, et al. Immunosuppressive effect of exosomes from mesenchymal stromal cells in defined medium on experimental colitis. Int J Stem Cells. 2019;12(3):440–8.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  154. 154.

    Lin T, Pajarinen J, Nabeshima A, Lu L, Nathan K, Jamsen E, et al. Preconditioning of murine mesenchymal stem cells synergistically enhanced immunomodulation and osteogenesis. Stem Cell Res Ther. 2017;8(1):277.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  155. 155.

    Sivanathan KN, Rojas-Canales DM, Hope CM, Krishnan R, Carroll RP, Gronthos S, et al. Interleukin-17A-induced human mesenchymal stem cells are superior modulators of immunological function. Stem Cells. 2015;33(9):2850–63.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  156. 156.

    Mathew SA, Chandravanshi B, Bhonde R. Hypoxia primed placental mesenchymal stem cells for wound healing. Life Sci. 2017;182:85–92.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  157. 157.

    Li B, Li C, Zhu M, Zhang Y, Du J, Xu Y, et al. Hypoxia-induced mesenchymal stromal cells exhibit an enhanced therapeutic effect on radiation-induced lung injury in mice due to an increased proliferation potential and enhanced antioxidant ability. Cell Physiol Biochem. 2017;44(4):1295–310.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  158. 158.

    Lee JH, Yoon YM, Lee SH. Hypoxic preconditioning promotes the bioactivities of mesenchymal stem cells via the HIF-1alpha-GRP78-Akt axis. Int J Mol Sci. 2017;18(6):1320.

    PubMed Central  Article  CAS  Google Scholar 

  159. 159.

    Lan YW, Choo KB, Chen CM, Hung TH, Chen YB, Hsieh CH, et al. Hypoxia-preconditioned mesenchymal stem cells attenuate bleomycin-induced pulmonary fibrosis. Stem Cell Res Ther. 2015;6(1):97.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  160. 160.

    Bader AM, Klose K, Bieback K, Korinth D, Schneider M, Seifert M, et al. Hypoxic preconditioning increases survival and pro-angiogenic capacity of human cord blood mesenchymal stromal cells in vitro. PLoS ONE. 2015;10(9):e0138477.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  161. 161.

    Beegle J, Lakatos K, Kalomoiris S, Stewart H, Isseroff RR, Nolta JA, et al. Hypoxic preconditioning of mesenchymal stromal cells induces metabolic changes, enhances survival, and promotes cell retention in vivo. Stem Cells. 2015;33(6):1818–28.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  162. 162.

    Chen X, Zhang F, He X, Xu Y, Yang Z, Chen L, et al. Chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells in type I collagen-hydrogel for cartilage engineering. Injury. 2013;44(4):540–9.

    PubMed  Article  PubMed Central  Google Scholar 

  163. 163.

    Breyner NM, Hell RC, Carvalho LR, Machado CB, Peixoto Filho IN, Valerio P, et al. Effect of a three-dimensional chitosan porous scaffold on the differentiation of mesenchymal stem cells into chondrocytes. Cells Tissues Organs. 2010;191(2):119–28.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  164. 164.

    Meng Q, Man Z, Dai L, Huang H, Zhang X, Hu X, et al. A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration. Sci Rep. 2015;5:17802.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  165. 165.

    You Y, Kobayashi K, Colak B, Luo P, Cozens E, Fields L, et al. Engineered cell-degradable poly(2-alkyl-2-oxazoline) hydrogel for epicardial placement of mesenchymal stem cells for myocardial repair. Biomaterials. 2020. https://doi.org/10.1016/j.biomaterials.2020.120356.

    Article  PubMed  PubMed Central  Google Scholar 

  166. 166.

    Chen S, Shi J, Zhang M, Chen Y, Wang X, Zhang L, et al. Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing. Sci Rep. 2015;5:18104.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  167. 167.

    Tsai TL, Manner PA, Li WJ. Regulation of mesenchymal stem cell chondrogenesis by glucose through protein kinase C/transforming growth factor signaling. Osteoarthritis Cartilage. 2013;21(2):368–76.

    PubMed  Article  PubMed Central  Google Scholar 

  168. 168.

    Khan M, Ali F, Mohsin S, Akhtar S, Mehmood A, Choudhery MS, et al. Preconditioning diabetic mesenchymal stem cells with myogenic medium increases their ability to repair diabetic heart. Stem Cell Res Ther. 2013;4(3):58.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  169. 169.

    Hildebrandt C, Buth H, Thielecke H. A scaffold-free in vitro model for osteogenesis of human mesenchymal stem cells. Tissue Cell. 2011;43(2):91–100.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  170. 170.

    Bartosh TJ, Ylostalo JH, Mohammadipoor A, Bazhanov N, Coble K, Claypool K, et al. Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc Natl Acad Sci U S A. 2010;107(31):13724–9.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  171. 171.

    Muttigi MS, Kim BJ, Kumar H, Park S, Choi UY, Han I, et al. Efficacy of matrilin-3-primed adipose-derived mesenchymal stem cell spheroids in a rabbit model of disc degeneration. Stem Cell Res Ther. 2020;11(1):363.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  172. 172.

    Bhang SH, Lee S, Shin JY, Lee TJ, Kim BS. Transplantation of cord blood mesenchymal stem cells as spheroids enhances vascularization. Tissue Eng Part A. 2012;18(19–20):2138–47.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  173. 173.

    Gothelf Y, Abramov N, Harel A, Offen D. Safety of repeated transplantations of neurotrophic factors-secreting human mesenchymal stromal stem cells. Clin Transl Med. 2014;3:21.

    PubMed  PubMed Central  Article  Google Scholar 

  174. 174.

    Berry JD, Cudkowicz ME, Windebank AJ, Staff NP, Owegi M, Nicholson K, et al. NurOwn, phase 2, randomized, clinical trial in patients with ALS: safety, clinical, and biomarker results. Neurology. 2019;93(24):e2294–305.

    PubMed  PubMed Central  Article  Google Scholar 

  175. 175.

    Guess AJ, Daneault B, Wang R, Bradbury H, La Perle KMD, Fitch J, et al. Safety profile of good manufacturing practice manufactured interferon gamma-primed mesenchymal stem/stromal cells for clinical trials. Stem Cells Transl Med. 2017;6(10):1868–79.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  176. 176.

    de Godoy MA, Saraiva LM, de Carvalho LRP, Vasconcelos-Dos-Santos A, Beiral HJV, Ramos AB, et al. Mesenchymal stem cells and cell-derived extracellular vesicles protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-beta oligomers. J Biol Chem. 2018;293(6):1957–75.

    PubMed  Article  PubMed Central  Google Scholar 

  177. 177.

    Wang JH, Liu XL, Sun JM, Yang JH, Xu DH, Yan SS. Role of mesenchymal stem cell derived extracellular vesicles in autoimmunity: a systematic review. World J Stem Cells. 2020;12(8):879–96.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  178. 178.

    Wang C, Borger V, Sardari M, Murke F, Skuljec J, Pul R, et al. Mesenchymal stromal cell-derived small extracellular vesicles induce ischemic neuroprotection by modulating leukocytes and specifically neutrophils. Stroke. 2020;51(6):1825–34.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  179. 179.

    Lai P, Chen X, Guo L, Wang Y, Liu X, Liu Y, et al. A potent immunomodulatory role of exosomes derived from mesenchymal stromal cells in preventing cGVHD. J Hematol Oncol. 2018;11(1):135.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  180. 180.

    Dreyer GJ, Groeneweg KE, Heidt S, Roelen DL, van Pel M, Roelofs H, et al. Human leukocyte antigen selected allogeneic mesenchymal stromal cell therapy in renal transplantation: the Neptune study, a phase I single-center study. Am J Transplant. 2020;20(10):2905–15.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  181. 181.

    McCradden MD, Stephenson EA, Anderson JA. Clinical research underlies ethical integration of healthcare artificial intelligence. Nat Med. 2020;26(9):1325–6.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  182. 182.

    Mak KK, Pichika MR. Artificial intelligence in drug development: present status and future prospects. Drug Discov Today. 2019;24(3):773–80.

    PubMed  Article  PubMed Central  Google Scholar 

  183. 183.

    Schneider P, Walters WP, Plowright AT, Sieroka N, Listgarten J, Goodnow RA Jr, et al. Rethinking drug design in the artificial intelligence era. Nat Rev Drug Discov. 2020;19(5):353–64.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  184. 184.

    Alberto AVP, da Silva Ferreira NC, Soares RF, Alves LA. Molecular modeling applied to the discovery of new lead compounds for P2 receptors based on natural sources. Front Pharmacol. 2020;11:01221.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  185. 185.

    Piazza I, Beaton N, Bruderer R, Knobloch T, Barbisan C, Chandat L, et al. A machine learning-based chemoproteomic approach to identify drug targets and binding sites in complex proteomes. Nat Commun. 2020;11(1):4200.

    PubMed  PubMed Central  Article  Google Scholar 

  186. 186.

    Adeshina YO, Deeds EJ, Karanicolas J. Machine learning classification can reduce false positives in structure-based virtual screening. Proc Natl Acad Sci U S A. 2020;117(31):18477–88.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  187. 187.

    Paul D, Sanap G, Shenoy S, Kalyane D, Kalia K, Tekade RK. Artificial intelligence in drug discovery and development. Drug Discov Today. 2020;26(1):80–93.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  188. 188.

    Bedard AC, Adamo A, Aroh KC, Russell MG, Bedermann AA, Torosian J, et al. Reconfigurable system for automated optimization of diverse chemical reactions. Science. 2018;361(6408):1220–5.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  189. 189.

    Norgeot B, Quer G, Beaulieu-Jones BK, Torkamani A, Dias R, Gianfrancesco M, et al. Minimum information about clinical artificial intelligence modeling: the MI-CLAIM checklist. Nat Med. 2020;26(9):1320–4.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  190. 190.

    Gonem S, Janssens W, Das N, Topalovic M. Applications of artificial intelligence and machine learning in respiratory medicine. Thorax. 2020;75(8):695–701.

    PubMed  Article  PubMed Central  Google Scholar 

  191. 191.

    Ranganath SH, Levy O, Inamdar MS, Karp JM. Harnessing the mesenchymal stem cell secretome for the treatment of cardiovascular disease. Cell Stem Cell. 2012;10(3):244–58.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  192. 192.

    Santamaria G, Brandi E, Vitola P, Grandi F, Ferrara G, Pischiutta F, et al. Intranasal delivery of mesenchymal stem cell secretome repairs the brain of Alzheimer’s mice. Cell Death Differ. 2021;28(1):203–18.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  193. 193.

    Sajeesh S, Broekelman T, Mecham RP, Ramamurthi A. Stem cell derived extracellular vesicles for vascular elastic matrix regenerative repair. Acta Biomater. 2020;113:267–78.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  194. 194.

    Qiu G, Zheng G, Ge M, Wang J, Huang R, Shu Q, et al. Mesenchymal stem cell-derived extracellular vesicles affect disease outcomes via transfer of microRNAs. Stem Cell Res Ther. 2018;9(1):320.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  195. 195.

    Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, et al. Reassessment of exosome composition. Cell. 2019;177(2):428–45.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  196. 196.

    Kalluri R, LeBleu VS. The biology, function, and biomedical applications of exosomes. Science. 2020;367(6478):eaau6977.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  197. 197.

    Pluchino S, Smith JA. Explicating exosomes: reclassifying the rising stars of intercellular communication. Cell. 2019;177(2):225–7.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  198. 198.

    Poggio M, Hu T, Pai CC, Chu B, Belair CD, Chang A, et al. Suppression of Exosomal PD-L1 induces systemic anti-tumor immunity and memory. Cell. 2019;177(2):414–27.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  199. 199.

    Doyle LM, Wang MZ. Overview of extracellular vesicles, their origin, composition, purpose, and methods for exosome isolation and analysis. Cells. 2019;8(7):727.

    CAS  PubMed Central  Article  Google Scholar 

  200. 200.

    Yin K, Wang S, Zhao RC. Exosomes from mesenchymal stem/stromal cells: a new therapeutic paradigm. Biomark Res. 2019;7:8.

    PubMed  PubMed Central  Article  Google Scholar 

  201. 201.

    Mendt M, Kamerkar S, Sugimoto H, McAndrews KM, Wu CC, Gagea M, et al. Generation and testing of clinical-grade exosomes for pancreatic cancer. JCI Insight. 2018;3(8):e99263.

    PubMed Central  Article  PubMed  Google Scholar 

  202. 202.

    Roberts-Dalton HD, Cocks A, Falcon-Perez JM, Sayers EJ, Webber JP, Watson P, et al. Fluorescence labelling of extracellular vesicles using a novel thiol-based strategy for quantitative analysis of cellular delivery and intracellular traffic. Nanoscale. 2017;9(36):13693–706.

    CAS  PubMed  Article  Google Scholar 

  203. 203.

    Saleh AF, Lazaro-Ibanez E, Forsgard MA, Shatnyeva O, Osteikoetxea X, Karlsson F, et al. Extracellular vesicles induce minimal hepatotoxicity and immunogenicity. Nanoscale. 2019;11(14):6990–7001.

    CAS  PubMed  Article  Google Scholar 

  204. 204.

    Conceicao M, Forcina L, Wiklander OPB, Gupta D, Nordin JZ, Vrellaku B, et al. Engineered extracellular vesicle decoy receptor-mediated modulation of the IL6 trans-signalling pathway in muscle. Biomaterials. 2021;266:120435.

    CAS  PubMed  Article  Google Scholar 

  205. 205.

    Liu M, Hu Y, Chen G. The antitumor effect of gene-engineered exosomes in the treatment of brain metastasis of breast cancer. Front Oncol. 2020;10:1453.

    PubMed  PubMed Central  Article  Google Scholar 

  206. 206.

    Tian X, Shen H, Li Z, Wang T, Wang S. Tumor-derived exosomes, myeloid-derived suppressor cells, and tumor microenvironment. J Hematol Oncol. 2019;12(1):84.

    PubMed  PubMed Central  Article  Google Scholar 

  207. 207.

    Stebbing J, Phelan A, Griffin I, Tucker C, Oechsle O, Smith D, et al. COVID-19: combining antiviral and anti-inflammatory treatments. Lancet Infect Dis. 2020;20(4):400–2.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  208. 208.

    Carlet J, Payen D, Opal SM. Steroids for sepsis and ARDS: this eternal controversy remains with COVID-19. Lancet. 2020;396(10259):e61-62.

    CAS  PubMed Central  Article  PubMed  Google Scholar 

  209. 209.

    Jiang L, Tang K, Levin M, Irfan O, Morris SK, Wilson K, et al. COVID-19 and multisystem inflammatory syndrome in children and adolescents. Lancet Infect Dis. 2020;20(11):e276–88.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  210. 210.

    Moll G, Drzeniek N, Kamhieh-Milz J, Geissler S, Volk HD, Reinke P. MSC therapies for COVID-19: importance of patient coagulopathy, thromboprophylaxis, cell product quality and mode of delivery for treatment safety and efficacy. Front Immunol. 2020;11:1091.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  211. 211.

    Jayaramayya K, Mahalaxmi I, Subramaniam MD, Raj N, Dayem AA, Lim KM, et al. Immunomodulatory effect of mesenchymal stem cells and mesenchymal stem-cell-derived exosomes for COVID-19 treatment. BMB Rep. 2020;53(8):400–12.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  212. 212.

    Leng Z, Zhu R, Hou W, Feng Y, Yang Y, Han Q, et al. Transplantation of ACE2(-) mesenchymal stem cells improves the outcome of patients with COVID-19 pneumonia. Aging Dis. 2020;11(2):216–28.

    PubMed  PubMed Central  Article  Google Scholar 

  213. 213.

    Haynes BF, Corey L, Fernandes P, Gilbert PB, Hotez PJ, Rao S, et al. Prospects for a safe COVID-19 vaccine. Sci Transl Med. 2020;12(568):eabe0948.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  214. 214.

    Shetty AK. Mesenchymal stem cell infusion shows promise for combating coronavirus (COVID-19)-induced pneumonia. Aging Dis. 2020;11(2):462–4.

    PubMed  PubMed Central  Article  Google Scholar 

Download references

Acknowledgements

The authors regret that it was not possible to include many interesting studies in the field due to limited space.

Funding

This work was supported by the National Key R&D Program of China (No. 2017YFE0131600), National Natural Science Foundation of China (Nos. 81870121, 81700825, 81671585, 82070176), Natural Science Foundation of Guangdong Province, China (Nos. 2019B020236004, 2019B151502006), Science and Technology Planning Project of Guangdong Province, China (No. 2017B020230004), and Science and Technology Program of Guangzhou, China (Nos. 201906010076, 201803040005).

Author information

Affiliations

Authors

Contributions

PLL, CH, and XD designed and wrote the review. TZ, ZNY, and JYW drafted the manuscript and prepared the figures. DQP helped to modify the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Xin Du or Chang He or Peilong Lai.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhou, T., Yuan, Z., Weng, J. et al. Challenges and advances in clinical applications of mesenchymal stromal cells. J Hematol Oncol 14, 24 (2021). https://doi.org/10.1186/s13045-021-01037-x

Download citation

Keywords

  • Mesenchymal stromal cells
  • Clinical applications
  • Heterogeneity
  • Artificial intelligence (AI)
  • Extracellular vesicles
  • COVID-19