Metabolism, metabolites, and macrophages in cancer
Journal of Hematology & Oncology volume 16, Article number: 80 (2023)
Tumour-associated macrophages (TAMs) are crucial components of the tumour microenvironment and play a significant role in tumour development and drug resistance by creating an immunosuppressive microenvironment. Macrophages are essential components of both the innate and adaptive immune systems and contribute to pathogen resistance and the regulation of organism homeostasis. Macrophage function and polarization are closely linked to altered metabolism. Generally, M1 macrophages rely primarily on aerobic glycolysis, whereas M2 macrophages depend on oxidative metabolism. Metabolic studies have revealed that the metabolic signature of TAMs and metabolites in the tumour microenvironment regulate the function and polarization of TAMs. However, the precise effects of metabolic reprogramming on tumours and TAMs remain incompletely understood. In this review, we discuss the impact of metabolic pathways on macrophage function and polarization as well as potential strategies for reprogramming macrophage metabolism in cancer treatment.
Macrophages are crucial immune cells in the body and play essential roles in both innate and adaptive immune responses . Along with other phagocytes, they form the initial line of defence by releasing proinflammatory cytokines, which contribute to the activation of the innate immune system and subsequent T- and B-cell responses . Macrophages can be polarized to acquire different phenotypes based on various stimuli. In the typical classification, proinflammatory macrophages acquire the M1 phenotype induced via lipopolysaccharide (LPS) and anti-inflammatory macrophages acquire the M2 phenotype induced via IL-4 or IL-13 [3, 4] (Table 1). In addition to the M2a phenotype induced by IL-4/IL-13, the M2 subset of macrophages can be further subclassified into different subtypes based on their specific functions. One of these subtypes consists of M2b macrophages, also known as regulatory macrophages. They are activated by immune complexes and TLR ligands and are involved in immune and inflammatory reactions, producing both pro- and anti-inflammatory cytokines. Another subtype consists of M2c macrophages, which are activated by glucocorticoids or IL-10 and primarily exert anti-inflammatory functions. M2d macrophages, also known as TAMs, are activated by TLR ligands and A2 adenosine receptor agonists, and they play crucial roles in regulating tumour progression, angiogenesis and metastasis [4,5,6,7]. In addition to the classical categorization of macrophages, there have been several other proposed classification systems based on different criteria. For example, Mosser et al. introduced a new classification method that categorizes macrophages into classically activated macrophages, wound-healing macrophages, and regulatory macrophages based on their homeostatic activities, namely, host defence, wound healing, and immune regulation, respectively . Macrophages within the tumour microenvironment (TME) are referred to as TAMs. As important immune cells that infiltrate the TME, TAMs are characterized by phenotypic plasticity and heterogeneity. Studies have confirmed that TAM heterogeneity is exhibited not only in different cancer patients but also in types of different cancers within the same patient, as well as in different stages of tumour development [9,10,11]. This heterogeneity reflects the ability of TAMs to respond to environmental stimuli, leading to polarization into phenotypes ranging from a proinflammatory (M1-like) to an anti-inflammatory (M2-like) types [12, 13]. Thus, TAM subsets exert diverse effects on tumourigenesis and tumour progression. For example, during the initiation stages of tumour formation, TAMs mainly play a proinflammatory role and suppress tumour development, although the evidence is still limited . As a tumour grows, macrophages in the TME are “educated” and acquire an M2-like phenotype through the action of Th2 cells. These cytotoxic macrophages then transition into tumour-supporting macrophages, promoting tumour progression . Additionally, specific subsets of TAMs have been associated with various processes such as oncogenesis, angiogenesis, vascularization, immunosuppression, metastasis, resistance to therapy, and poorer clinical outcome [15,16,17]. However, TAMs can also exhibit tumouricidal functions by mediating tumour phagocytosis and promoting anti-tumour immunity [18, 19]. Notably, TAMs can play dual roles depending on the context. While they can exert a tumour-promoting effect and be associated with poor prognosis in certain cancers, such as breast cancer , lung cancer , and pancreatic cancer , they can also exert anti-tumour effects on colon cancer . Furthermore, the localization of TAMs within the TME influences their functions. Generally, TAMs located in hypoxic areas or close proximity to blood vessels exert proangiogenic effects. On the other hand, TAMs that infiltrate the tumour front have been found to play an anti-tumourigenic role specifically in colon cancer (Fig. 1) [23, 24].
After malignant transformation, intratumour angiogenesis is crucial for further tumour progression. In 1971, Folkman et al. initially proposed a correlation between tumour growth and angiogenesis . Initially, tumour angiogenesis was believed to be induced only by tumour cells. However, Staton et al. discovered that TAMs also regulate angiogenesis through factors such as hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), and tissue factor (TF) [26, 27]. In addition, other inflammatory cytokines secreted by TAMs have also been found to promote neovascularization through multiple signalling pathways . Furthermore, TAMs have been implicated in facilitating tumour invasion and metastasis [29, 30]. Therefore, the possibility of targeting TAMs has attracted increasing attention in recent years, and TAMs have been depleted using colony-stimulating factor-1 (CSF-1)/CSF-1R inhibitors and by re-educating TAMs towards an M1-like phenotype through signalling pathways involving CD40, CD47, phosphoinositide 3-kinase (PI3K) and toll-like receptor (TLR) [31,32,33,34,35].
In addition to the aforementioned methods, metabolic reprogramming has also been progressively adopted for regulating macrophage polarization  (Table 2). Tumour cells exhibit peculiar metabolic processes that support their nutrient and energy requirements. Although metabolic alterations in tumours are widely studied, tumour-specific metabolic characteristics of tumour cells have not been established. The Warburg effect (aerobic glycolysis) is one of the best characterized metabolic changes in tumour cells . Even under aerobic conditions, tumour cells reprogramme glucose metabolism to utilize glycolysis instead of mitochondrial oxidative phosphorylation (OXPHOS) . In the past, researchers thought that glycolysis was the main source of ATP that maintains tumour cell growth . However, in glycolysis, each glucose molecule produces two ATP molecules, while mitochondrial OXPHOS produces approximately 30 ATP molecules . Therefore, aerobic glycolysis is not the main source of ATP synthesis in tumour cells and many tumours obtain energy through glucose oxidation [40,41,42,43]. Nevertheless, studies have shown that high glycolytic rates can provide metabolic precursors for biomass production [44, 45]. In aerobic glycolysis, glucose-derived pyruvate is converted to lactate by lactate dehydrogenase (LDH), which allows the regeneration of NADH, which maintains glycolysis . Moreover, other fuels, such as amino acids, fatty acids, and proteins, can also provide energy for tumour growth . This altered tumour cell metabolism has a profound impact on the TME and may significantly affect the metabolism and polarization of TAMs. However, the mechanisms underlying TAM metabolism and polarization remain poorly characterized, and further investigation is needed. Several studies have confirmed that different metabolites promote TAM polarization into different phenotypes with diverse functions [8, 47]. Therefore, clarifying the mechanisms underlying metabolic processes and macrophage polarization and exploring potential strategies targeting TAMs are necessary. This review discusses the cross talk between the factors involved in metabolism and macrophage polarization, and summarizes a viable strategy for targeting TAMs by reprogramming metabolism.
Metabolic influences in the TME
Metabolism changes in cells within the TME exert a profound impact not only on tumour progression but also on the functionality of immune cells. Understanding metabolic influences in immune cell function is crucial for the development of novel immunotherapeutic strategies. Obesity has been identified as a risk factor for the development of various types of tumours . Previous studies on obesity and cancer have predominantly focused on the tumours themselves [49, 50]. However, Ringel et al. demonstrated that a high-fat diet (HFD) promotes colorectal tumour growth in a T cell-dependent manner . Mechanistically, tumour cells can increase fatty acid uptake from a HFD and alter fatty acid distribution within tumours, resulting in impaired CD8+ T cell infiltration and function. Inhibition of the metabolic reprogramming of tumour cells restores the anti-tumour function of CD8+ T cells. In addition to CD8+ T cell, metabolism also plays a regulatory role in other immune cells within the TME . For instance, Ma et al. found that bile acid can promote the infiltration of CXCR6+ natural killer T (NKT) cells and enhance their anti-tumour activity in liver cancer . Aberrant glycolysis in tumours leads to increased production of lactic acid in the TME. Studies have confirmed that excessive lactic acid disrupts the metabolism of human cytotoxic T lymphocytes (CTLs), inhibiting their proliferation and reducing cytokine production . This phenomenon can be prevented by inhibitors of lactic acid production. Moreover, in a low-glucose environment, T cells are stimulated to differentiate from effector T cells to acquire a Foxp3+ regulatory (Treg) phenotype, which exerts a tumour-promoting effect . Additionally, increased glycolytic metabolism in tumour cells promotes the secretion of tumour granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF), which further promotes the recruitment of MDSCs and inhibit T cells activity .
Recently, immune checkpoint blockades (ICB) have become widely used in immunotherapy for treating malignant tumours. Studies have found that obesity significantly regulates the expression of the immune checkpoint molecules PD-1 and PD-L1 . Moreover, it has been reported that obese or overweight patients with melanoma, non-small cell lung cancer (NSCLC), or renal cell carcinoma show better responses to ICB immunotherapy [57,58,59]. Therefore, modulating immune cell metabolism or targeting metabolic vulnerabilities in cancer cells may help enhance anti-tumour immune responses and increase the effectiveness of immunotherapies.
Cross talk between metabolism and macrophage polarization
As an important part of the innate immune system, macrophages exhibited high plasticity and could effectively respond to various stimuli. M1 macrophages are characterized by enhanced glycolysis, high level of glutathione, increased expression of ferritin, elevated expression of cyclooxygenase (COX) 2, low expression of COX1, augmented activity of inducible nitric oxide synthase (iNOS) 2, and decreased activity of arginase 1 (Arg1). However, M2 macrophages are depicted with enhanced fatty acid oxidation (FAO), low expression of ferritin, reduced levels of glutathione, diminished COX2 production, elevated COX1 production, weak iNOS activity, and increased Arg1 activity.
TAMs can enhance hypoxic and aerobic glycolysis in mouse subcutaneous tumours and in patients with NSCLC by secreting tumour necrosis factor-alpha (TNF-α), whereas increased AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor gamma (PPARγ) co-activator 1-α levels in TAMs facilitate tumour hypoxia [9, 60]. Macrophage polarization is typically associated with glucose metabolism. Activated macrophages are essentially glycolytic cells, with a clear cut-off between classic activation and the alternative pathway. Interestingly, M1 macrophage activation through LPS/IFN-γ, listeria monocytogenes, thioglycolate, TLR-2, -3, -4, or -9 resulted in similar flux distribution patterns towards anaerobic glycolysis, regardless of the activated pathway [61, 62](Fig. 2). However, stimulation via alternative pathways has minor metabolic effects. It has been observed in animal models that the molecular basis of the differences between these two types of behaviour involves a switch in the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK2) from the liver type-PFK2 to the more active ubiquitous PFK2 isoenzyme, which responds to HIF-1α activation and increases fructose-2,6-bisphosphate concentration and glycolytic flux . The opposite is true when macrophages are activated by interleukin (IL)-4 and IL-13, which promote the alternative phenotype, often termed M2 macrophages, which are associated with tissue repair and humoral immunity, anti-inflammatory cytokine production, reduced expression of MHC-II, and antigen presentation . They exhibit enhanced OXPHOS and much lower rates of glycolysis, and have no detectable PFKFB3, expressing PFKFB1 instead. In human hepatocellular carcinoma, Chen et al. purified monocytes/macrophages from peripheral blood and found that PFKFB3 in TAMs not only modulated the cellular metabolic switch but also mediates the increased expression of PD-L1 by activating the nuclear factor-kappa B (NF-κB) signalling pathway in these cells . The association between OXPHOS and the production of anti-inflammatory cytokines and glycolysis associated with the production of proinflammatory cytokines remains unclear. However, PPARγ co-activator-1β (PGC-1β) is a transcriptional co-activator that promotes oxidative metabolism, notably by upregulating the expression of genes involved in FAO in the M2 macrophage phenotype, which is a profound increase in the entire programme of fatty acid metabolism, including the uptake and oxidation of fatty acids and mitochondrial biogenesis, depending on the activation of signal transducer and activator of transcription 6 (STAT6) (Fig. 3). Notably, in the B16 melanoma tumour model, STAT6 has been demonstrated to induce M2 macrophage polarization and mediate the suppression of TRIM24 expression in M2 macrophages, contributing to the induction of an immunosuppressive tumour niche . In addition, in the pentose phosphate pathway (PPP), the carbohydrate kinase-like (CARKL) protein, also known as sedoheptulose kinase (SHPK), plays a key role in regulating macrophage metabolism and can influence macrophage polarization, which catalyses the production of sedoheptulose-7-phosphate (S7P) as a rate-limiting step for balancing metabolic intermediates of non-oxidative PPP and glycolysis . SHPK is downregulated upon LPS stimulation both in vitro and in vivo, and downregulation of SHPK is essential for M1-like metabolic reprogramming. Furthermore, SHPK antagonizes LPS-induced cytokine production (i.e., TNF-α and IL-6) by inhibiting NF-κB. Therefore, the negative effect of SHPK activity on M1 macrophage function raises the possibility that changes in glucose metabolism influence the inflammatory properties of M1 macrophages (Fig. 2). LPS-induced M1 macrophages also displayed increased glycolysis and decreased oxygen consumption (oxygen consumption rate, OCR) . This contrasts sharply with IL-4-polarized M2-like macrophages, whose metabolic profile is similar to that of unpolarized macrophages. Two important metabolites generated by oxidative PPP are nicotinamide adenine dinucleotide phosphate (NADPH) and ribulose-5-phosphate (Ru5P) [68, 69]. If the cellular need for NADPH exceeds nucleotide biosynthesis, Ru5P passes into the non-oxidative arm of the PPP to generate F6P and G3P, which enter glycolysis again. M1 macrophages drive several processes that require a high amount of NADPH, notably NADPH oxidase-dependent respiratory burst and glutathione biosynthesis, to buffer reactive oxygen species (ROS) . ROS can further activate NF-kB signalling to promote PD-L1 transcription and release of immunosuppressive chemokines from TAMs . In triple-negative breast cancer models, ROS inducers such as paclitaxel, glutathione synthesis inhibitor, and buthionine sulphoximine can significantly promote ROS accumulation and elevate PD-L1 transcription both in vitro and in vivo experiments . In contrast, ectopic expression of SHPK reduces the oxidative PPP flux and promotes an oxidative state (increased GSSG and NAD +) characteristic of M2-like polarization. Maintenance of a high NADH/NAD + ratio induced by LPS stimulation may enhance NF-κB binding activity and favour M1 macrophage differentiation . In traumatic brain injury (TBI), delayed NADPH oxidase 2 (NOX2) activation induces NF-κB activation, amplifies neuroinflammation, enhances M1 polarization, and increases myeloid-mediated neurotoxicity . Furthermore, NOX2-dependent ROS production occurs upstream of ATM activation, which is required for ionizing radiation-elicited macrophage activation and for macrophage reprogramming towards a proinflammatory phenotype after treatment with IFN-γ, LPS, or chemotherapeutic agents through the regulation of mRNA levels and post-translational modifications of IFN regulatory factor 5 (IRF5) . A metabolic transition towards glycolysis, reminiscent of the Warburg effect, occurs in LPS-stimulated macrophages. Mitochondrial OXPHOS was reduced, but anaerobic glycolysis was enhanced. LPS elevates the transcription level of HIF-1α via the mitogen-activated protein kinase (MAPK) and NF-κB pathways, with a decrease in the mRNA levels of TLR4-dependent prolyl hydroxylase (PHD) mRNA levels. The LPS-induced decrease in OXPHOS leads to the accumulation of intermediate metabolites in the tricarboxylic acid cycle, especially succinic acid . Succinic acid can be transferred from the mitochondria to the intracellular space, inhibit the activity of the PHD enzyme, and increase HIF-1α by promoting its stability. The depletion of HIF-1α in macrophages results in decreased production of IL-1β, which unsensitized mice to LPS-induced endotoxic shock with a lower mortality rate than wild-type mice.
Macrophages often accumulate in large numbers in areas of hypoxia, a prominent feature of various inflamed and diseased tissues, including malignant tumours, atherosclerotic plaques, myocardial infarcts, synovia of joints with rheumatoid arthritis, healing wounds, and sites of bacterial infection, where hypoxia affects the function of macrophages . A recent study found that macrophages exert enhanced phagocytic clearance of apoptotic cells (efferocytosis) upon chronic physiological hypoxia . In this process, macrophages flux glucose into PPP and promote NADPH production, which further induces phagolysosomal maturation and redox homeostasis to enhance efferocytosis. Thus, the macrophages play an important role in maintaining body homeostasis through efferocytosis under physiological hypoxic conditions. As an important molecule that regulates macrophage function under hypoxic conditions, HIF-1α can promote glycolysis and PPP by inducing the expression of related enzymes and transcriptional effectors, thereby affecting the biological functions of macrophages. For example, in hepatocellular carcinoma, the glycolytic enzyme pyruvate kinase M2 (PKM2), induced by hepatoma cell-derived fibronectin 1, can regulate macrophage glycolysis in a HIF-1α-dependent manner . PKM2 is a protein kinase that regulated aerobic glycolysis [79, 80]. Follistatin-like protein 1 (FSTL1) promotes PKM2 phosphorylation and nuclear translocation via direct binding, induces PKM2-dependent glycolysis, and promotes M1 polarization . Simultaneously, HIF-1α promotes the expression of proinflammatory genes in macrophages, enhances phagocytosis, affects the production of anti-microbial peptides and granzyme, and plays an important role in inflammatory response. Loss of HIF-1α reduces the bactericidal activity of macrophages and the secretion of proinflammatory cytokines. It has been found that HIF-1α can contribute to the synthesis of iNOS and to the other hypoxia response elements (HRE)-dependent transcriptional activity when stimulated synergistically by LPS or hypoxia . Zhang and colleagues have demonstrated that M2 macrophages enhance 3-phosphoinositide-dependent protein kinase 1 (PDPK1)-mediated phosphoglycerate kinase 1 (PGK1) threonine (T) 243 phosphorylation in tumour cells by secreting IL-6 . This phosphorylation facilitates a PGK1-catalysed reaction towards glycolysis by altering substrate affinity. In addition, PGK1 T243 phosphorylation correlates with PDPK1 activation, IL-6 expression, and macrophage infiltration in human glioblastoma (GBM) and correlates with the malignancy and prognosis of human GBM . As has been descripted, hypoxia and inflammation are critical factors that influence the hepatocellular carcinoma microenvironment. TAMs secrete more IL-1β under moderate hypoxic conditions because of the increased stability of HIF-1α, which induces necrotic debris in hepatocellular carcinoma cells. Necrotic debris further induce IL-1β secretion via TLR4/TRIF/NF-κB signalling. However, overexpression of HIF-1α leads to epithelial–mesenchymal transition (EMT) and metastasis in hepatocellular carcinoma cells . Furthermore, hepatocellular carcinoma-derived IL-8 promotes a pro-oncogenic inflammatory microenvironment by inducing M2-type TAMs and indirectly promoting EMT . Additionally, hypoxic conditions can suppress forkhead box O1 (FoxO1) expression, which positively regulates MHC-II genes by binding to the promoter region of Ciita, the master activator of MHC-II genes. Yang et al. used FoxO1 conditional knockout mice to confirm that loss of FoxO1 in TAMs results in reduced MHC-II expression . In the TME with high lactate content, prolonged lactic acidosis induces the differentiation of monocytes into macrophages with a phenotype that includes tumour-promoting and inflammatory characteristics (VEGFhi CXCL8+IL-1β+). In vitro activation of macrophages at pH 6.8 in vitro enhanced the IL-4-driven phenotype and contributed to prostate carcinogenesis . These effects of lactate require its metabolism and are associated with HIF-1α stabilization. The expression of lactate-induced genes is dependent on autocrine macrophage-CSF (M-CSF) consumption . The tumour-derived soluble molecule succinate activates succinate receptor 1 (SUCNR1) signalling to polarize macrophages into TAMs and promote tumour cell migration and invasion as well as metastasis by the SUCNR1-triggered PI3K-HIF-1α axis . High concentrations of lactate within the anaerobic tumour environment activate the mechanistic target of rapamycin complex 1 (mTORC1), which subsequently suppresses the transcription factor EB (TFEB)-mediated expression of the macrophage-specific vacuolar ATPase subunit ATP6V0d2, which targets HIF-2α but not HIF-1α, for lysosome-mediated degradation . Hypoxia can also induce lactate production via glycolysis, which acts as a precursor for stimulating histone lactylation. Histone lactylation has different temporal dynamics from acetylation. In the late phase of M1 macrophage polarization, increased histone lactylation induces homeostatic genes involved in wound healing, including Arg1 . Previous studies have confirmed that hypoxic TAMs play an important role in promoting tumour angiogenesis [24, 92]; however, whether metabolic changes can reverse this effect is ambiguous. Wenes et al. found elevated expression of REDD in hypoxic TAMs from Lewis lung carcinomas, orthotopic E0771 breast cancer, and spontaneous PyMT mammary tumours . To further understand these mechanisms, they constructed chimeric mice and revealed that REDD1 can hinder the glycolysis of TAMs and enhance their proangiogenic function by inhibiting mTOR. Furthermore, REDD1-deficient TAMs compete with tumour endothelial cells to utilize glucose, which stabilizes endothelial cells and blocks abnormal blood vessel formation. Therefore, TAM metabolism also plays regulatory roles in tumour angiogenesis.
Lipidomic studies have confirmed that lipid metabolism is related to macrophage activation [94, 95]. However, when excess cholesterol is absorbed, abnormal cholesterol metabolism in macrophages leads to several pathological changes. Using index and transcriptional single-cell sorting, researchers have revealed a novel lipid-associated macrophage subset, which is characterized by lipid receptor Trem2 expression in both mice and humans during obesity . Mechanistically, they used Trem2-deficient mice to confirm that Trem2 plays an important role in preventing adipocyte hypertrophy and in regulating systemic cholesterol levels. Thus, lipid-associated macrophages may be effective targets in for metabolic diseases. Abundant endoplasmic reticulum and free cholesterol in macrophages promotes the esterification of cholesterol acyltransferase 1 (ACAT1), which in turn leads to the production of more free cholesterol and increases the inflammatory signals induced by lipid rafts, especially TLRs and NF-κB. This signalling pathway causes changes in the lipid metabolism of macrophages. TLR agonists promote the biosynthesis of atypical arachidonic acid from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and produce anti-inflammatory lipid regulators such as resolvin and protectin during tissue repair by M2 macrophages. Notably, Endogenous oxidized lipids can simultaneously promote OXPHOS and aerobic glycolysis in LPS-stimulated phagocytes . Studies have also demonstrated that fatty acid absorption and oxidation were significantly increased in IL-4 stimulated M2 macrophages and inhibited in M1 macrophages [98, 99] (Fig. 2). The polarization of human macrophages is related to the levels of glycosphingolipid regulators, sphingosine and ceramide. The arachidonic acid pathway can describe the biosynthesis of proinflammatory lipid mediators, such as prostaglandin E2 (PGE2) and PGD2, in the inflammatory response, which is one of the ligands of the liver X receptor (LXR) in the nucleus. LXR prevents arachidonic acid from remodelling TLR4 response elements, thereby inhibiting TLR4-activated macrophages. Therefore, the anti-inflammatory effects of arachidonic acid are partly dependent on the LXR pathway. However, there are significant differences in the expression of arachidonic acid pathway-related enzymes between M1 and M2 macrophages in humans, which express high level of cyclooxygenase 2 (COX2) and low levels of COX1, leukotriene a4 hydrolase (LTA4H), and arachidonate 5-lipoxygenase (ALOX5) upon stimulation with IFN-γ and/or LPS, whereas ALOX15 and COX1 increased markedly after treatment of macrophages with IL-4. The synthesis of arachidonic acid is catalysed by 24-dehydrocholesterol reductase (DHCR24), which inhibits the expression of DHCR24 in mice fed a high-fat diet, leading to M1-type activation of macrophages. 5-Lipoxygenase (5-LO) is key to the synthesis of leukotrienes, which are potent proinflammatory lipid mediators involved in chronic inflammatory diseases, including cancer. The expression and activity of 5-LO in TAMs were reduced upon co-culture with dying cancer cells through Mer tyrosine kinase (MerTK)-dependent recognition of apoptotic cancer cells, which can be repressed by the proto-oncogene c-Myb at the transcriptional level . Notably, blockade of MerTK resulted in the accumulation of apoptotic cells within tumours and triggered a type I interferon response. Treatment of tumour-bearing mice with the anti-MerTK antibody stimulated T cell activation and synergized with anti-PD-1 or anti-PD-L1 therapy. Mechanistically, extracellular ATP acts via P2X7R to enhance the transport of extracellular cyclic guanosine monophosphate–adenosine monophosphate (cGAMP) into macrophages and subsequent stimulator of interferon gene (STING) activation . Treg cells promote M2-like TAMs by repressing the CD8+ T cell-IFN-γ axis by blocking the activation of sterol regulatory element-binding protein 1 (SREBP1)-mediated fatty acid synthesis . Cytosine–guanine dinucleotide (CpG) activation engenders a metabolic state that requires fatty acid oxidation (FAO) and the shunting of tricarboxylic acid (TCA) cycle intermediates for de novo lipid biosynthesis. This integration of metabolic inputs is underpinned by carnitine palmitoyl transferase 1A and adenosine tri-phosphate citrate lyase, which together impart macrophages with anti-tumour potential capable of overcoming inhibitory CD47 on cancer cells . Macrophages from both human and murine tumour tissues are enriched with lipids owing to increased lipid uptake. TAMs express elevated levels of the scavenger receptor CD36, accumulate lipids, and use FAO instead of glycolysis for energy . High levels of FAO promote mitochondrial OXPHOS, ROS, Janus kinase 1 (JAK1) phosphorylation, and Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP1) dephosphorylation, leading to STAT6 activation and transcription of genes that regulate TAM generation and function . Peroxisome proliferator-activated receptor (PPAR) is a ligand-dependent transcription factor that acts as a fatty acid receptor to regulate glucose and lipid metabolism, and can be further divided into three subgroups: PPARα, PPARδ, and PPARγ . PPARα and PPARγ are widely expressed in human and mouse monocytes and macrophages and inhibit the expression of proinflammatory genes in macrophages. Therefore, PPAR is generally believed to prevent M1 polarization, and its effect on M2 polarization has gradually been discovered in recent years. PPARγ controls the expression of genes encoding molecules that mediate various aspects of lipid metabolism, including storage, lipolysis, and cholesterol efflux . PPARγ, which affects M2 polarization, mainly promotes fatty acid omega-oxidation and mitochondrial generation at the transcriptional level, and can couple with PGC-1β to directly regulate the production of Arg1, a hallmark of M2 macrophages. Similarly, knockout of PPARγ in macrophages both in vivo and in vitro inhibited the activation of M2 macrophages, and decreased Arg1 production [108, 109]. Mice fed a high-fat diet that specifically knocked out PPARγ in myeloid cells were more prone to obesity and insulin resistance owing to damage to mitochondrial function. Caspase-1 promotes TAM differentiation by cleaving PPARγ at Asp64, which translocates into the mitochondria where it directly interacts with medium-chain acyl-CoA dehydrogenase (MCAD). This binding event attenuates MCAD activity and inhibits fatty acid oxidation, thereby leading to the accumulation of lipid droplets and promotion of TAM differentiation . IL-4 can also induce PPARδ transcription and act synergistically with STAT6 to promote M2 activation. Unlike PPARγ, PPARδ is not required for the oxidative metabolism. It is worth mentioning that the co-regulation of STAT6 and PPARγ affects the transcription of genes related to fatty acid oxidation, leading to the polarization of macrophages into M2, and the metabolic level shifts from glycolysis to fatty acid oxidation. Notably, rosiglitazone, a PPARγ agonist, can partially decrease C–C motif chemokine ligand 2 (CCL2) secretion by tumour cells and reduce the infiltration of TAMs to the irradiated tumour site, thereby delaying tumour regrowth after radiotherapy, suggesting that the combination of the PPARγ agonist rosiglitazone with radiotherapy can enhance the effectiveness of radiotherapy . PGC-1β, a transcriptional co-stimulator of PPAR, increases the expression of genes related to fatty acid oxidation and promotes OXPHOS. During M2 polarization by IL-4, fatty acids metabolism was significantly improved, as well as the oxidation and absorption of fatty acid and the number of mitochondria, mainly because IL-4 activates the transcription factor STAT6 and further induces the production of PGC-1β. Intracellular overexpression of PGC-1β promotes M2 polarization and alleviate macrophage-related inflammatory responses. In contrast, conditional knockout of PGC-1β inhibited intracellular OXPHOS and M2 function, significantly promoting the M1 inflammatory response activated by LPS. Similarly, prostaglandin E2 (PGE2) can elevated mitochondrial OXPHOS by inhibiting PPARγ and inducing alterative macrophage polarization . Monoacylglycerol lipase (MGLL) can lead to lipid overload in TAMs, which functionally inhibits CB-2 cannabinoid receptor-dependent tumour progression in inoculated and genetic cancer models. Mechanistically, MGLL deficiency promotes CB-2/TLR4-dependent macrophage activation, which further suppresses the function of tumour-associated CD8+ T cells . Ovarian cancer cells promote membrane cholesterol efflux and lipid raft depletion in macrophages. Increased cholesterol efflux promotes IL-4-mediated reprogramming, including the inhibition of IFN-γ-induced gene expression, which reverts the tumour-promoting functions of TAMs and reduces tumour progression [107, 114].
Amino acid metabolism
In macrophages, intracellular metabolism of L-Arg is mainly regulated by two enzymes: iNOS and Arg1. iNOS catalyses the conversion of L-Arg into NO and L-citrulline. NO plays a bactericidal role, and L-citrulline is utilized in the urea cycle. Arg1 catalyses L-Arg to produce ornithine and uridine, and promote the formation of polyamines in collagen synthesis, cell proliferation, and tissue remodelling. Arg1 activity can be significantly elevated in M2 macrophage upon stimulation with IL-4 in mice, but similar results have not been found in human macrophages. Elevated amino acid catabolism is common in several cancers. Glioblastoma can produce large amounts of branched-chain ketoacids (BCKAs), which can be taken up and reaminated into branched-chain amino acids (BCAAs) by TAMs. Exposure to BCKAs can reduce the phagocytic activity of macrophages . SLC7A5, an important transporter, has been demonstrated to mediate the uptake of amino acids in tumours and T cells, and it has been confirmed that SLC7A5-mediated metabolic reprogramming plays a major role in macrophages polarization . SLC7A5 promotes the release of proinflammatory cytokines from macrophages by inducing leucine influx and upregulating glycolytic reprogramming via the mTORC1 signalling pathway. Ornithine decarboxylase (ODC) is the rate-limiting enzyme in polyamine biosynthesis and restricts M1 macrophage activation in gastrointestinal (GI) infections, which augments epithelial injury-associated colitis and colitis-associated carcinogenesis (CAC) by impairing M1 responses that stimulate epithelial repair, anti-microbial defence, and anti-tumour immunity . Arginase 2 (ARG2) drives neuroblastoma cell proliferation via regulation of arginine metabolism, which polarizes infiltrating monocytes to an M1 macrophage phenotype, releasing IL-1β and TNF-α in an RAC-α serine/threonine protein kinase (AKT)-dependent manner . Serine is a substrate for nucleotide, NADPH, and glutathione (GSH) synthesis. In macrophages, serine is required for optimal LPS induction of IL-1β mRNA expression, but not for inflammasome activation .
Metabolites that regulate macrophage polarization
Changes in macrophage metabolism are accompanied by intermediate metabolic alterations. Specifically, the metabolites produced during in TCA cycle play important roles in the modulation of macrophages (Table 3).
Succinate is an intermediate product of the TCA cycle , and it is significantly increased in response to LPS stimulation . LPS increased levels of succinate played crucial roles in stabilizing HIF-1α and promoting IL-1β production in proinflammatory macrophages by impairing PHD activity. This finding suggests that succinate directly regulates the HIF-1α pathway, thereby influencing macrophage function. Furthermore, in vitro and in vivo studies have shown that tumour-derived succinate in the TME can activate SUCNR1, leading to the polarization of macrophages into TAMs. Additionally, succinate promotes tumour cell migration and invasion. High levels of succinate and SUCNR1 expression are associated with poor clinical outcome in lung cancer .
Itaconate has been shown to be produced by macrophages after LPS stimulation . LPS-treated macrophages exhibit high expression of immune-responsive gene 1 (IRG1), which catalyses the decarboxylation of cis-aconitate to produce itaconate . Silencing the IRG1 gene in macrophages significantly reduces itaconic acid production during bacterial infections, highlighting the important role of IRG1 in regulating immune defence and itaconate production. However, Lampropoulou et al. demonstrated that itaconate exerted anti-inflammatory effects by inhibiting succinate dehydrogenase (SDH) production, leading to increased succinate accumulation and decreased levels of mitochondrial reactive oxygen species (ROS), which subsequently inhibited the release of proinflammatory cytokines . Additionally, itaconate and its derivative 4-octyl itaconate (OI) inhibited NLRP3 inflammasome activation, thereby limiting inflammation in a urate-induced peritonitis model . Similarly, Hoyle et al. found that OI the derivative 4OI and dimethyl fumarate (DMF) effectively inhibited the production of proinflammatory cytokines in murine BMDMs, mixed glia and organotypic sliced hippocamp cultures in response to LPS . Moreover, itaconate alkylated cysteine residues on the protein Kelch-like ECH-associated protein 1 (KEAP1) in both mouse and human macrophages, leading to the negative modulation of the expression of nuclear factor 2 (Nrf2) [126, 127], a transcription factor essential for activating antioxidant and anti-inflammatory responses. Thus, itaconate may exert anti-inflammatory effects in a Nrf2-dependent manner. Itaconate and its derivative dimethyl itaconate (DI) also exerted anti-inflammatory effects through an Nrf2-independent pathway . They induce electrophilic stress to inhibit the IκBζ-ATF3 inflammatory axis. Additionally, the itaconate derivative OI has been found to decrease the activity of the glycolytic enzyme GAPDH, thereby blocking glycolytic flux, reducing aerobic glycolysis, and preventing proinflammatory TAM activation . Furthermore, itaconate suppressed M2 macrophage polarization , as it inhibited JAK1 and STAT6 activation.
Researchers have discovered that itaconate promotes tumour growth by mediating cross talk between macrophages and cancer cells in peritoneal tumours . Itaconate enhanced OXPHOS-driven ROS expression and induced MAPK activation, mediated through ROS signalling in tumour cells. Blocking itaconate and IRG1 significantly inhibited tumour progression and may thus be an effective therapeutic strategy. In colorectal cancer, itaconate downregulated PPARγ expression and increased the secretion of anti-inflammatory cytokines by M2 macrophages, thereby promoting tumourigenesis . A recent study demonstrated that itaconate suppressed CD8+ T cell proliferation, and blocking itaconate restored anti-tumour immunity in mouse models of melanoma . Moreover, itaconate inhibitors synergized with immune checkpoint inhibitors, resulting in a greater anti-tumour effect on melanoma.
α-Ketoglutarate (α-KG) is generated in various metabolic pathways. Previous studies have shown that α-KG is produced through the oxidative decarboxylation of isocitrate by isocitrate dehydrogenases (IDHs) . A recent study found that glutamine deprivation in mouse BMDMs inhibited the expression of M2-like marker genes but promoted the expression of M1-like marker genes after LPS stimulation. However, the precise mechanisms by which glutamine metabolism regulates macrophage polarization remain unclear. Liu et al. discovered that α-KG, generated from glutamine, promoted M2 polarization through the Jmjd3 signalling pathway . Moreover, α-KG inhibited M1 macrophage function by suppressing the NF-κB pathway in a PHD-dependent manner. α-KG effectively activated the PHD enzyme, leading to a significant reduction in IKKβ activation, which is necessary for NF-κB pathway activation. As mentioned earlier, succinate stabilizes HIF-1α, whereas α-KG destabilizes it [47, 75]. Therefore, an elevated α-KG/succinate ratio may promote M2 macrophage polarization, while a reduced α-KG/succinate ratio may facilitate M1 macrophage reprogramming by targeting HIF-1α-mediated aerobic glycolysis. In mouse BMDMs, type I interferon (IFNβ) increased IRG1 expression, promoting the production of itaconate and succinate while inhibiting α-KG production . Downregulation of the α-KG/succinate ratio suppressed M2 macrophage polarization through the JMJD3/IRF4-mediated pathway. Thus, IFNβ plays a significant role in regulating macrophage polarization by controlling the α-KG/succinate ratio. Although the involvement of glutamine-derived α-KG in regulating M2 macrophage activation has been established, the specific mechanisms remain unclear. Zhou et al. revealed that after IL-4 stimulation, glutaminolysis promoted α-KG accumulation and reprogrammed M2 polarization through the SENP1-Sirt3 axis in BMDMs . The SENP1-Sirt3 axis deacetylated glutamate dehydrogenase 1 (GLUD1), an acetylated protein in the mitochondria, and activated GLUD1 induced α-KG accumulation, promoting M2 macrophage polarization .
Citrate has been shown to induce pro- or anti-inflammatory macrophage polarization through different mechanisms . Previous studies have demonstrated that citrate derived from mitochondria promoted the activation of proinflammatory macrophages. The mitochondrial citrate carrier (CIC) facilitated the export of citrate from mitochondria in LPS-activated macrophages, leading to increased HIF-1α expression. HIF-1α, in turn, upregulated IRG1, resulting in itaconate production . Inhibition of CIC suppressed citrate accumulation and enhanced mitochondrial oxidation by blocking the itaconate shunt, ultimately causing a switch from M1 to M2 BMDM polarization after LPS stimulation. Moreover, in human macrophage cells derived from histiocytoma, the proinflammatory cytokines TNF-α and IFN-γ were required for mitochondrial CICs production of nitric oxide and prostaglandin .
In contrast, Covarrubias et al. discovered that IL-4 activates the Akt and mTORC1 signalling pathways. Activation of Akt-mTORC1 pathway promoted the conversion of citrate into acetyl-CoA through the activation of ATP-citrate lyase (ACLY). This process increased histone acetylation and promoted the expression of M2 genes, ultimately leading to M2 macrophage activation .
Metabolite-regulated macrophage polarization influences cancer outcome
As a major component of immune cells in the TME, TAMs play pivotal roles in tumour progression. Strategies targeting TAMs focus mainly on TAM deletion, inhibition of TAM recruitment, and reprogramming of TAM polarization [15, 141, 142]. However, the therapeutic effects of these approaches are still not ideal, and there is an urgent need for new effective therapies targeting TAMs in tumour treatment. Recent studies have highlighted a role for metabolic reprogramming in controlling macrophage function and polarization, leading to various clinical experiments aimed at regulating macrophages (as summarized in Table 4). Because a limited number of reviews are available on this topic, we summarize the metabolic reprogramming of macrophages during cancer treatment in Table 5.
Regulating TAM function through aerobic glycolysis
Tumour metastasis is a leading cause of treatment failure and recurrence. Several cancer-related features contributes to the development of the pre-metastatic niche, including inflammation , angiogenesis , immunosuppression , and reprogramming . Among the various immune cells in the pre-metastatic niche, macrophages have gained significant attention [147, 148]. Targeting macrophages shows potential for suppressing tumour metastasis. However, the precise mechanisms underlying the regulation of macrophage polarization and function in the pre-metastatic niche have remained unclear. Morrissey and colleagues have revealed that tumour-derived exosomes polarized macrophages into an immunosuppressive subtype via metabolic reprogramming, which increased glucose uptake through the NF-κB pathway, elevated NOS2 expression, and inhibited mitochondrial OXPHOS, which favoured the conversion of pyruvate into lactate in the lung cancer context. These outcomes were confirmed both in vitro in F4/80+ peritoneal macrophage experiments and in vivo in animal experiments . Notably, increased lactate levels established a feedback metabolism to the NF-κB pathway and elevated PD-L1 expression in macrophages. Ultimately, this resulted in tumour progression and metastasis. Therefore, reprogramming macrophage metabolism by regulating tumour-derived exosomes might be an effective anti-tumour therapeutic strategy.
Zinc finger E-box binding homeobox 1 (Zeb1), a transcription factor, has been demonstrated to reprogramme TAMs to become immunosuppressive M2-like TAMs in human breast cancer samples. Zeb1 induced aerobic glycolysis in TAMs, leading to increased lactate production, which forms an acidic environment that promotes tumour progression and metastasis . In a hypoxic environment, Zeb1 promoted the expression of glycolytic-related enzymes through the PI3K/AKT signalling pathway. Depletion of Zeb1 inhibited PI3K/AKT activity and aerobic glycolysis, which may indicate that Zeb1 depletion is potential therapeutic strategy for breast cancer because it led to attenuate aerobic glycolysis and reprogrammed TAM polarization. Endocrine therapy has led to major advances in oestrogen receptor (ER)-positive breast cancer treatment. However, resistance to endocrine therapy remains a challenge. TAMs play important roles in inducing endocrine therapy resistance, but the specific mechanism underlying its effect remains unclear . Niu et al. discovered that overexpression of sodium/glucose co-transporter 1 (SGLT1) enhanced glycolysis in ER-positive breast cancer cells and promoted M2-like TAM polarization mediated through the HIF1α pathway. In turn, M2-like TAMs upregulated SGLT1 expression via EGFR/PI3K/Akt signalling, leading to endocrine therapy resistance in ER-positive breast cancer cells . Therefore, targeting SGLT1 may be an effective treatment for overcoming endocrine therapy resistance in breast cancer and reprogramming TAM polarization. Triple-negative breast cancer, a distinct variant of breast cancer with a unique pathology, shows poor responses to immunotherapy because of the high lactic acid metabolism rate and high antioxidant levels in the TME . New nanodrugs have been developed to polarize TAMs into the anti-tumour TAMs by reprogramming TAM metabolism, thereby enhancing the anti-tumour effects of TAMs [154, 155]. Macrophage migration inhibitory factor (MIF) is a well-characterized immunosuppressive factor that is secreted by immune cells and plays an important role in tumour immune escape by binding to its receptor CD74 [156, 157]. Previous studies have shown that MIF-CD74 inhibitors restored the anti-tumour immune function of macrophages and dendritic cells in metastatic melanoma . Recently, Azevedo et al. revealed that blockade of the MIF-CD74 signalling pathway reprogrammed the metabolic pathway by decreasing lactate production and promoting M1-like macrophage conversion in the TME. Moreover, MIF-CD74 blockade combined with anti-CTLA-4 therapy elevated CD8+ T cell infiltration and inhibited melanoma progression and metastasis . Hepatocellular carcinoma-derived polarization-promoting factors promoted TAM polarization to the M2-like phenotype by activating the Wnt2b/β-catenin/c-Myc signalling pathway, which enhanced TAM glycolysis. This effect was blocked by the TLR9 agonist CpG ODN, which inhibited Wnt2b/β-catenin pathway activation and suppressed the M2 polarization of TAMs in hepatocellular carcinoma samples, ultimately reversing the tumour-promoting effects of the TAMs both in vitro and in vivo .
Previous studies have verified a relationship between mTOR signalling and TAM repolarization , and a combination of mTOR inhibitors and anti-angiogenic therapy has achieved good results in clinical experiments . However, the specific molecular mechanisms have not yet been elucidated. Chen et al. designed a liposomal system including the mTOR inhibitor rapamycin and the anti-angiogenic drug regorafenib, and found that this liposome effectively reprogrammed M2-like TAMs to M1-like TAMs by remodelling glycolytic metabolism and reducing lactic acid production via the mTOR pathway. This effect was confirmed with both CT26 colon cancer cells and a colorectal tumour model . Therefore, anti-angiogenesis and mTOR inhibition may co-regulate the repolarization.
In addition to being mediated by cellular factors, metabolic reprogramming is also activated by direct cell–cell contact in pancreatic ductal adenocarcinoma (PDA). Zhang and colleagues used a PDA macrophage co-culture system, that is, an “orthotopic” PDA syngeneic mouse model, and human PDA specimens to confirm that PDA tumour cells promoted the reprogramming of the M1-like cell phenotype into the M2-like cell phenotype through direct interaction with M1-like macrophage but not M2-like macrophages, a process that was mediated by GARP and integrin αV/β8, inducing DNA methylation and downregulating glucose metabolism and OXPHOS gene expression . Inhibition of GARP or integrin reversed this outcome and restored the anti-tumour effect of the M1-like macrophages.
Regulating TAM function through lipid metabolism
Recently, researchers have found that abnormal lipid metabolism and TAMs lead poor prognosis in pancreatic cancer. Cao et al. found that MGLL was highly expressed in pancreatic cancer, while the function endocannabinoid receptor-2 (CB-2), which can regulate macrophage polarization, was also dysregulated in TAMs . The group synthesized a nanoplatform that simultaneously delivered MGLL siRNA and CB-2 siRNA to inhibit free fatty acid production in the TME and reprogramme TAMs to polarize into a tumour-inhibiting M1-like TAMs. Tumour cells have been reported to promote cholesterol efflux and reduce lipid rafts formation in macrophages . Compared to naïve macrophages, TAMs are associated with increased expression of genes related to cholesterol metabolism and cholesterol efflux. Cholesterol depletion in macrophages induces IL-4-mediated macrophage activation and polarization through the STAT6-PI3K pathway, and these IL-4-mediated macrophages exerted immunosuppressive functions and promoted tumour progression . Therefore, the cholesterol metabolism pathway is likely a novel target for reprogramming TAM polarization and function. AB-hydrolase containing 5 (ABHD5) functions as a co-activator of adipose triglyceride lipase and plays a critical role in the lipolysis of triglycerides into diglycerides and free fatty acids . Miao et al. found that ABHD5 is highly expressed in colorectal cancer-related TAMs, which inhibited spermidine synthase (SRM)-dependent spermidine production by suppressing C/EBPε expression and counteracting the anti-tumour effect of TAM-derived spermidine on colorectal cancer . Therefore, the ABHD5/SRM/spermidine metabolic pathway is a novel therapeutic strategy for colorectal cancer treatment.
Regulating TAM function through TCA cycle metabolism
RNA-sequencing (RNA-seq) analysis revealed that the gene expression of protein kinase RNA-like endoplasmic reticulum kinase (PERK) favours the polarization of M2 macrophages and is associated with macrophage metabolism, including glutamine metabolism, amino acid synthesis, lipid metabolism, and OXPHOS . Raines and colleagues discovered that PERK mediated mitochondrial respiration and FAO to meet M2 macrophage energy demands. PERK stimulated α-KG production in M2 macrophages by activating phosphoserine aminotransferase 1 (PSTA1), which was necessary for M2 macrophage metabolic reprogramming, and supported JMJD3-mediated histone demethylation to promote immunosuppressive gene expression in macrophages. Furthermore, inhibition of the PERK signalling pathway inhibited glutamine utilization and α-KG concentration in M2 macrophages, reduced immunosuppressive TAM activity, and suppressed tumour progression. Therefore, the PERK signalling pathway may be an effective target for the treatment of cancers by reprogramming macrophage metabolism.
Slit2, a secretory glycoprotein, has been found to inhibit breast cancer progression ; however, the specific mechanism remains unknown. Kaul and colleagues used a spontaneous mammary tumour virus promoter–polyoma middle T antigen (PyMT) breast cancer mouse model and found that Slit2 promoted BMDMs polarization towards an anti-tumour phenotype and enhanced the anti-tumour immune response by increasing glycolysis and reducing FAO in BMDMs via the mTOR signalling pathway. Moreover, Slit2 treatment reduced the α-KG-to-succinic acid ratio and changed mitochondrial respiration metabolites in macrophage-derived from healthy human blood that had been treated with breast cancer patient plasma . These finding suggest that, Slit2 may be an important therapeutic target for breast cancer because it reprogrammes macrophage metabolism.
Recently, new metabolic supramolecular nanotherapeutics loaded with a TLR7/8 agonist and an FAO inhibitor were synthesized, and they effectively inhibited the TCA cycle and upregulated the glycolytic metabolic pathway of TAMs in breast cancer. Ultimately, M2-like TAMs are reprogrammed to be M1-like TAMs, significantly reduced the tumour progression and metastasis rate . RIPK3 has been demonstrated to play an important role in activating the pyruvate dehydrogenase complex E3 subunit and increasing anaerobic glycolysis . Considering that M1 macrophage polarization is associated with aerobic glycolysis, RIPK3 may contribute to M1 polarization of proinflammatory macrophages. Researchers have found that RIPK3 is downregulated in hepatocellular carcinoma cells and can induce M2-like TAM polarization and recruitment by activating the PPAR pathway to reprogramme fatty acid metabolism . In addition, the upregulation of RIPK3 or ablation of FAO switched TAMs from the M2-like to M1-like phenotype and may be a potential method of tumour immunotherapy and metabolism-targeted therapy.
Regulating TAM function through amino acid metabolism
The metabolism of L-arginine changes during macrophage polarization, and two of the three L-arginine catalytic enzymes, iNOS and arginase 1, have been well studied. However, the third metabolic product of L-arginine creatine which functions in the immune system, remains unclear. Creatine uptake, which is mediated by Slc6a8, reprogrammed macrophage polarization by inhibiting the IFN-γ-JAK-STAT1 signalling pathway and suppressing the expression of the immune effector molecule, IFN-γ. Additionally, it led to upregulated IL-4-STAT6 pathway activation and promoted of immune suppressor production . Therefore, creatine metabolism plays a key role in macrophage polarization and the immune response and may emerge as an important therapeutic target for treatments mediated via macrophage repolarization.
Methionine is an essential amino acid, and researchers have revealed that methionine and methionine adenosylmethionine (MAT) enzymes play significant roles in tumourigenesis and tumour progression [174, 175]. However, whether methionine and MAT enzymes are associated with macrophage polarization remains unclear. Zhang at el. found that the expression of the MAT enzyme MAT2A was significantly upregulated in CD14+ monocytes purified from gastric cancer patient’s tumour tissues, and methionine metabolism promoted M2 macrophage polarization through MAT2A action, while MAT2A induced the epigenetic activation of RIP1 expression. Inhibition of MAT2A hindered M2 macrophage polarization . Hence, targeting the MAT2A-RIP1 pathway may be a meaningful therapeutic strategy to reprogramme TAM metabolism and induce TAM polarization.
Regulating TAM function through phosphoinositide metabolism
Phosphoinositides constitute a very small percentage of membrane phospholipids and play important roles in signalling modulation . PIP2 and PIP3 have been shown to regulate signal transduction through the PI3K/Akt signalling pathway . Tumour necrosis factor α-induced protein 8-like 1 (TIPE1) has been demonstrated to be highly expressed in isolated peritoneal macrophages, BMDMs and cultured THP1 cells, in which it promoted M2 macrophage polarization by directly binding to PIP2 and PIP3, regulating their metabolic pathways . In vitro and in vivo, TIPE1 blockade in macrophages inhibits PI3K/Akt pathway activity and abrogated the progression and metastasis of melanoma and liver cancer cells. Therefore, phosphoinositide signalling and metabolism may be effectively changed through TAM reprogramming and acquisition of an anti-tumour phenotype.
Recently, dramatic advances have been made in tumour immunotherapy. For example, immune checkpoint blockade therapy has been successful in reducing many types of solid tumour [180,181,182], and chimeric antigen receptor (CAR)-T therapy has also shown promising effects in the treatment of haematologic malignancies [183, 184]. However, the wide application of CAR-T cell therapy is limited due to severe toxicity, such as cytokine release syndrome (CRS) mediated by cytokines derived from macrophages . Therefore, targeting TAMs is a necessary and promising strategy for tumour immunotherapy. TAM infiltration has been associated with poor prognosis in many malignant tumours [30, 186], but little is known about the effects of TAM metabolic changes on tumour progression. Metabolic reprogramming leads to functional modifications and repolarization of TAMs. Increased glycolysis, decreased FAO, and a reprogrammed TCA cycle promoted the repolarization of TAMs into acquiring the proinflammatory phenotype. Metabolites produced during metabolic reprogramming such as lactate, α-KG, and succinic acid, also regulated macrophage activation. Therefore, it is critical to understand the cross talk among the factors involved in metabolic alterations and macrophage function.
In recent years, radiotherapy and chemotherapy have shown good efficacy in the treatment of malignant tumours. However, radiotherapy and chemotherapy resistance remain great challenges to effective cancer treatment. Many studies have revealed that abnormal lipid metabolism is associated with resistance to radiotherapy and chemotherapy [187,188,189]. Moreover, CPT1A has been shown to be highly expressed in radioresistant cancer cells and can increase the FAO rate, while inhibition of fatty acid synthesis or targeting CPT1A attenuated radioresistance and decreased radiation-mediated ERK activation [190, 191]. Radiation promoted macrophage differentiation into different phenotypes in a dose-dependent manner. For example, high doses of irradiation (20 Gy) triggered macrophage polarization into the acquisition of an anti-inflammatory phenotype, whereas low-dose irradiation (2 Gy) skewed macrophages to an anti-tumour phenotype [192, 193]. Therefore, different doses may trigger different metabolic reprogramming processes. Thus, it may be important to explore the metabolic reprogramming of TAMs after treating them with different radiation doses.
Availability of data and materials
Hypoxia inducible factor-1α
Vascular endothelial growth factor
Inducible nitric oxide synthase
Fatty acid oxidation
Non-small cell lung cancer
Tumour necrosis factor-alpha
AMP-activated protein kinase
Peroxisome proliferator-activated receptor gamma
Nuclear factor-kappa B
Signal transducer and activator of transcription 6
Pentose phosphate pathway
Nicotinamide adenine dinucleotide phosphate
Reactive oxygen species
Traumatic brain injury
NADPH oxidase 2
IFN regulatory factor 5
Mitogen-activated protein kinase
Hypoxia response elements
3-Phosphoinositide-dependent protein kinase 1
Phosphoglycerate kinase 1
Forkhead box O1
Succinate activates succinate receptor 1
Mechanistic target of rapamycin complex 1
Transcription factor EB
Pyruvate kinase M2
Follistatin-like protein 1
Cholesterol acyltransferase 1
Ligands of the liver X receptor
Leukotriene a4 hydrolase
Mer tyrosine kinase
Cyclic guanosine monophosphate–adenosine monophosphate
Stimulator of interferon genes
Sterol regulatory element-binding protein 1
Fatty acid oxidation
Janus kinase 1
Src homology 2 domain-containing protein tyrosine phosphatase 1
Peroxisome proliferator-activated receptor
Medium-chain acyl-CoA dehydrogenase
C–C motif chemokine ligand 2
Branched-chain amino acids
Serine/threonine protein kinase
Zinc finger E-box binding homeobox 1
Sodium/glucose co-transporter 1
Migration inhibitory factor
Pancreatic ductal adenocarcinoma
AB-hydrolase containing 5
Protein kinase RNA-like endoplasmic reticulum kinase
Phosphoserine aminotransferase 1
Tumour necrosis factor α-induced protein 8-like 1
Chimeric antigen receptor
Cytokine release syndrome
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This work was supported by the National Natural Science Foundation of China (82173174 and 81803051 to C. Li, 82073335 to J. Wang), Natural Science Foundation of Beijing Municipality (7232207 to C. Li), intramural funding by the Beijing University Third Hospital (BYSY2022044 to C. Li), and Special fund of the National Clinical Key Specialty Construction Program, P. R. China (2021).
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Li, M., Yang, Y., Xiong, L. et al. Metabolism, metabolites, and macrophages in cancer. J Hematol Oncol 16, 80 (2023). https://doi.org/10.1186/s13045-023-01478-6
- Tumour-associated macrophages
- Tumour microenvironment
- Metabolism reprogramming